Microviridae sp. ctOsc38

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2WAR3|A0A5Q2WAR3_9VIRU Major capsid protein OS=Microviridae sp. ctOsc38 OX=2656698 PE=3 SV=1
MM1 pKa = 7.39VNDD4 pKa = 4.52PEE6 pKa = 4.66TVPSKK11 pKa = 10.96YY12 pKa = 9.89PADD15 pKa = 3.63FRR17 pKa = 11.84LVEE20 pKa = 4.34LGEE23 pKa = 4.09WDD25 pKa = 3.88TTTGIYY31 pKa = 10.06SEE33 pKa = 4.59NEE35 pKa = 3.69VPKK38 pKa = 10.87ALGSALDD45 pKa = 3.79YY46 pKa = 10.77KK47 pKa = 10.66RR48 pKa = 11.84RR49 pKa = 11.84PQDD52 pKa = 3.54EE53 pKa = 4.91LPLKK57 pKa = 10.47AVQQ60 pKa = 3.47

Molecular weight: 6.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2WBY3|A0A5Q2WBY3_9VIRU Uncharacterized protein OS=Microviridae sp. ctOsc38 OX=2656698 PE=4 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84GRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84MSKK9 pKa = 9.51RR10 pKa = 11.84RR11 pKa = 11.84SRR13 pKa = 11.84RR14 pKa = 11.84LFTRR18 pKa = 11.84TAKK21 pKa = 10.04RR22 pKa = 11.84VHH24 pKa = 6.13RR25 pKa = 11.84RR26 pKa = 11.84NGGGRR31 pKa = 11.84VMRR34 pKa = 11.84GGVRR38 pKa = 11.84LL39 pKa = 3.57

Molecular weight: 4.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1448

39

554

241.3

26.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.671 ± 1.954

0.967 ± 0.458

5.663 ± 0.705

5.87 ± 1.518

4.489 ± 0.719

9.323 ± 0.98

2.003 ± 0.566

2.97 ± 0.571

4.42 ± 1.169

7.251 ± 0.467

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.417 ± 0.602

3.729 ± 0.519

5.594 ± 0.694

3.729 ± 0.67

8.287 ± 1.931

5.594 ± 0.699

5.525 ± 1.041

5.594 ± 0.69

1.45 ± 0.409

3.453 ± 0.863
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski