Proteome-pI 2.0 - Proteome Isoelectric Point Database

Macrococcus caseolyticus (strain JCSC5402)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Staphylococcaceae; Macrococcus; Macrococcus caseolyticus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 2059 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|B9E761|B9E761_MACCJ Endonuclease MutS2 OS=Macrococcus caseolyticus (strain JCSC5402) OX=458233 GN=mutS2 PE=3 SV=1

MM1 pKa = 7.46INVTVLVNEE10 pKa = 4.9DD11 pKa = 3.54GQVTEE16 pKa = 4.87FTMEE20 pKa = 3.88GHH22 pKa = 7.19ADD24 pKa = 3.91SAPHH28 pKa = 6.38GQDD31 pKa = 3.5LVCAGASAVVFGSMNAVMGLTDD53 pKa = 4.17EE54 pKa = 5.47RR55 pKa = 11.84PDD57 pKa = 3.62IDD59 pKa = 3.8YY60 pKa = 11.14ADD62 pKa = 4.23DD63 pKa = 4.06GGYY66 pKa = 7.93FTARR70 pKa = 11.84VVDD73 pKa = 4.65LNNKK77 pKa = 7.04EE78 pKa = 3.98AQLIIQSMLVSLQTIEE94 pKa = 4.95AEE96 pKa = 3.76YY97 pKa = 10.45GQYY100 pKa = 10.44IKK102 pKa = 11.09LNYY105 pKa = 8.91KK106 pKa = 10.0

Molecular weight: 11.53 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|B9ECF5|B9ECF5_MACCJ Uncharacterized protein OS=Macrococcus caseolyticus (strain JCSC5402) OX=458233 GN=MCCL_plsG0005 PE=4 SV=1

MM1 pKa = 6.87KK2 pKa = 9.12TANINTRR9 pKa = 11.84KK10 pKa = 9.1FRR12 pKa = 11.84LMYY15 pKa = 10.12NFNHH19 pKa = 6.36IVDD22 pKa = 3.55ITEE25 pKa = 4.22LEE27 pKa = 4.12EE28 pKa = 3.87NGYY31 pKa = 7.6MFRR34 pKa = 11.84TFSNEE39 pKa = 3.51DD40 pKa = 3.32YY41 pKa = 11.32VNVGNGIVIRR51 pKa = 11.84RR52 pKa = 11.84HH53 pKa = 5.58RR54 pKa = 11.84DD55 pKa = 3.02NNNFLNNTVRR65 pKa = 11.84MLPLAEE71 pKa = 4.98LLSQIKK77 pKa = 7.43TVRR80 pKa = 11.84NLQGQIIYY88 pKa = 10.05KK89 pKa = 9.81RR90 pKa = 11.84PNRR93 pKa = 11.84DD94 pKa = 2.5RR95 pKa = 11.84HH96 pKa = 4.82IRR98 pKa = 11.84KK99 pKa = 9.14VNYY102 pKa = 9.26VRR104 pKa = 11.84KK105 pKa = 10.04DD106 pKa = 3.24RR107 pKa = 11.84LKK109 pKa = 11.01NSS111 pKa = 3.39

Molecular weight: 13.4 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
2059	0	2059	642868	37	2045	312.2	35.28

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
6.511 ± 0.064	0.662 ± 0.016	5.756 ± 0.05	7.133 ± 0.068	4.479 ± 0.046	6.272 ± 0.051	2.39 ± 0.028	8.603 ± 0.059	7.377 ± 0.057	9.227 ± 0.065

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.984 ± 0.03	5.093 ± 0.047	3.185 ± 0.031	3.583 ± 0.036	3.822 ± 0.034	5.765 ± 0.039	5.665 ± 0.05	6.789 ± 0.042	0.721 ± 0.016	3.982 ± 0.035

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski