Spirochaeta africana (strain ATCC 700263 / DSM 8902 / Z-7692)

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Spirochaetaceae; Spirochaeta; Spirochaeta africana

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2766 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9UG03|H9UG03_SPIAZ Putative ATPase of the PP-loop superfamily implicated in cell cycle control OS=Spirochaeta africana (strain ATCC 700263 / DSM 8902 / Z-7692) OX=889378 GN=Spiaf_0340 PE=4 SV=1
MM1 pKa = 6.77MWKK4 pKa = 9.78SLKK7 pKa = 10.44SSFVGVLLVLCIVVSAGSCSNLFEE31 pKa = 6.21AGEE34 pKa = 4.24RR35 pKa = 11.84TVALQLEE42 pKa = 4.76VRR44 pKa = 11.84APALFPAADD53 pKa = 3.35ARR55 pKa = 11.84GRR57 pKa = 11.84TILPDD62 pKa = 3.05AAMAEE67 pKa = 4.57VVLLEE72 pKa = 4.42HH73 pKa = 6.89NGASLQQHH81 pKa = 6.79HH82 pKa = 7.18LPLTYY87 pKa = 10.69DD88 pKa = 3.34PVLQEE93 pKa = 4.15FHH95 pKa = 6.62ATLLLEE101 pKa = 4.99DD102 pKa = 3.98VPAGNSHH109 pKa = 6.58ILAVEE114 pKa = 3.99IQDD117 pKa = 3.95EE118 pKa = 4.44EE119 pKa = 4.53GAPLQEE125 pKa = 4.18AQAQVSVVSGEE136 pKa = 4.32TTSVTLILFPHH147 pKa = 6.9TDD149 pKa = 3.42LVQPGDD155 pKa = 3.49LAEE158 pKa = 4.85DD159 pKa = 3.86MEE161 pKa = 4.77EE162 pKa = 4.11TVPPGGYY169 pKa = 9.23WILAYY174 pKa = 10.59SLAAGQDD181 pKa = 3.63YY182 pKa = 11.07ALEE185 pKa = 4.7AEE187 pKa = 4.28AGEE190 pKa = 4.79DD191 pKa = 3.12IEE193 pKa = 6.37LRR195 pKa = 11.84IQLPDD200 pKa = 3.52GSFLPAGEE208 pKa = 4.33ITWEE212 pKa = 4.03QQEE215 pKa = 4.37TGTVYY220 pKa = 10.84LYY222 pKa = 10.49IFNRR226 pKa = 11.84GDD228 pKa = 3.35DD229 pKa = 4.98DD230 pKa = 4.64IPVLLEE236 pKa = 3.8LTASGAVEE244 pKa = 3.88YY245 pKa = 9.9TIGDD249 pKa = 3.62TGPAGGVVFYY259 pKa = 11.0DD260 pKa = 2.95QGEE263 pKa = 4.57VVNGWRR269 pKa = 11.84YY270 pKa = 10.6LEE272 pKa = 4.19ATPAGWSGGEE282 pKa = 4.28DD283 pKa = 3.29PVAAWGDD290 pKa = 3.49DD291 pKa = 3.57SLIGTEE297 pKa = 4.29TNFGFGKK304 pKa = 10.59LNTLTIISRR313 pKa = 11.84LQDD316 pKa = 3.26WEE318 pKa = 4.41DD319 pKa = 3.26GQYY322 pKa = 11.13AARR325 pKa = 11.84LSGDD329 pKa = 3.86YY330 pKa = 10.96AVGEE334 pKa = 4.07YY335 pKa = 10.64DD336 pKa = 4.88DD337 pKa = 4.41WFLPSLEE344 pKa = 4.29EE345 pKa = 4.17LFEE348 pKa = 4.98LRR350 pKa = 11.84AQQDD354 pKa = 3.29IVGGFPIEE362 pKa = 4.24GNEE365 pKa = 4.07DD366 pKa = 3.84IYY368 pKa = 10.89WSSSEE373 pKa = 5.04FFDD376 pKa = 6.22DD377 pKa = 4.86PDD379 pKa = 4.55DD380 pKa = 4.5PPEE383 pKa = 3.84EE384 pKa = 4.38HH385 pKa = 6.72SWVLGFIAGEE395 pKa = 3.83SSEE398 pKa = 4.02WLKK401 pKa = 11.1EE402 pKa = 3.87FDD404 pKa = 3.83DD405 pKa = 3.9TRR407 pKa = 11.84VRR409 pKa = 11.84PVRR412 pKa = 11.84AFRR415 pKa = 11.84SEE417 pKa = 4.05LPTYY421 pKa = 10.5AVVYY425 pKa = 9.91HH426 pKa = 6.42PNEE429 pKa = 4.37GQGDD433 pKa = 4.12PPVDD437 pKa = 4.0PYY439 pKa = 10.88HH440 pKa = 6.61YY441 pKa = 10.48EE442 pKa = 3.82PGEE445 pKa = 4.19PVTLPDD451 pKa = 3.64AGEE454 pKa = 4.47LYY456 pKa = 10.57RR457 pKa = 11.84EE458 pKa = 4.8GYY460 pKa = 9.78EE461 pKa = 3.88FAGWNSEE468 pKa = 3.85PDD470 pKa = 3.57GTGDD474 pKa = 3.42QYY476 pKa = 11.61IAIGNITMPQGNLILYY492 pKa = 9.46AEE494 pKa = 4.29WAEE497 pKa = 3.94IEE499 pKa = 4.07PVYY502 pKa = 10.52TIGDD506 pKa = 3.63TGPAGGIIFYY516 pKa = 10.95VDD518 pKa = 3.96DD519 pKa = 3.72TDD521 pKa = 5.82QYY523 pKa = 12.05DD524 pKa = 2.82GWTYY528 pKa = 11.42LEE530 pKa = 4.62AAPAGWDD537 pKa = 3.85DD538 pKa = 4.25PGEE541 pKa = 4.49PDD543 pKa = 4.38PEE545 pKa = 5.68DD546 pKa = 5.02PLRR549 pKa = 11.84QWDD552 pKa = 4.66DD553 pKa = 3.33DD554 pKa = 4.1TFSYY558 pKa = 10.85SAVTTEE564 pKa = 4.03TAIGTGKK571 pKa = 10.32EE572 pKa = 3.55NTAAIVTALGGDD584 pKa = 4.09GKK586 pKa = 9.38TGMAAQLAAEE596 pKa = 4.3LEE598 pKa = 4.38ITNGEE603 pKa = 4.03TDD605 pKa = 3.38YY606 pKa = 11.79SDD608 pKa = 3.01WFLPSRR614 pKa = 11.84DD615 pKa = 4.12EE616 pKa = 4.59LNEE619 pKa = 4.3LYY621 pKa = 9.34MQEE624 pKa = 4.44AIIDD628 pKa = 3.91GLYY631 pKa = 10.73VSAGWYY637 pKa = 8.41YY638 pKa = 10.14WSSSTADD645 pKa = 3.91EE646 pKa = 4.12SWAWSQDD653 pKa = 3.22FFNGDD658 pKa = 3.27QSVTNGKK665 pKa = 9.73NISTYY670 pKa = 10.08IRR672 pKa = 11.84VRR674 pKa = 11.84PIRR677 pKa = 11.84RR678 pKa = 11.84FF679 pKa = 3.22

Molecular weight: 74.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9UMJ8|H9UMJ8_SPIAZ DNA polymerase I OS=Spirochaeta africana (strain ATCC 700263 / DSM 8902 / Z-7692) OX=889378 GN=polA PE=3 SV=1
MM1 pKa = 7.18QLRR4 pKa = 11.84DD5 pKa = 3.52IVTPLIILPAAAVLSWVFPQYY26 pKa = 9.36NTAFIQVGLQFVALLFFLAGILLSILYY53 pKa = 10.1HH54 pKa = 6.55RR55 pKa = 11.84GRR57 pKa = 11.84VLAALMLMLLTAAAFASPVVLLLEE81 pKa = 5.16PYY83 pKa = 8.23QTTMVYY89 pKa = 10.5RR90 pKa = 11.84LLQLSMPLLLCVIAISPRR108 pKa = 11.84RR109 pKa = 11.84RR110 pKa = 11.84ILDD113 pKa = 3.66PASLVWLASIALLVFAVWAVPNTEE137 pKa = 3.77QAPRR141 pKa = 11.84LLQPLFTLQLLPFLPPQLLPQALPQISIAMALFGLLCVILVEE183 pKa = 4.56STYY186 pKa = 10.88HH187 pKa = 6.88RR188 pKa = 11.84DD189 pKa = 3.49PSLSLAWICAYY200 pKa = 10.47AAVCLALITLEE211 pKa = 4.31TPARR215 pKa = 11.84AILWFTAAGAVLAVGLIQHH234 pKa = 6.49AHH236 pKa = 5.66RR237 pKa = 11.84MAFIDD242 pKa = 3.75ALTRR246 pKa = 11.84IPNRR250 pKa = 11.84RR251 pKa = 11.84ALDD254 pKa = 3.64EE255 pKa = 3.93QLSRR259 pKa = 11.84LRR261 pKa = 11.84SRR263 pKa = 11.84YY264 pKa = 9.43AIAMVDD270 pKa = 2.93IDD272 pKa = 4.29HH273 pKa = 7.03FKK275 pKa = 11.22AFNDD279 pKa = 3.67THH281 pKa = 4.85GHH283 pKa = 5.73KK284 pKa = 10.19MGDD287 pKa = 3.0QALRR291 pKa = 11.84KK292 pKa = 6.83TASHH296 pKa = 5.95LRR298 pKa = 11.84EE299 pKa = 3.58VDD301 pKa = 2.93GGGKK305 pKa = 8.01VFRR308 pKa = 11.84YY309 pKa = 9.89GGEE312 pKa = 3.8EE313 pKa = 3.79FTILFPGKK321 pKa = 6.75TARR324 pKa = 11.84EE325 pKa = 4.2AGRR328 pKa = 11.84HH329 pKa = 4.35CQALRR334 pKa = 11.84EE335 pKa = 4.33RR336 pKa = 11.84IAGEE340 pKa = 3.71PFFFRR345 pKa = 11.84GSNRR349 pKa = 11.84PRR351 pKa = 11.84KK352 pKa = 9.04LRR354 pKa = 11.84KK355 pKa = 9.28AEE357 pKa = 3.73KK358 pKa = 9.43HH359 pKa = 4.85RR360 pKa = 11.84RR361 pKa = 11.84RR362 pKa = 11.84PTRR365 pKa = 11.84KK366 pKa = 9.33RR367 pKa = 11.84EE368 pKa = 4.04ASDD371 pKa = 3.27SLQITVSIGVAAQGDD386 pKa = 4.02RR387 pKa = 11.84ASTSARR393 pKa = 11.84VLQLADD399 pKa = 3.17QALYY403 pKa = 9.82HH404 pKa = 6.25AKK406 pKa = 10.26KK407 pKa = 10.11NGRR410 pKa = 11.84NRR412 pKa = 11.84VIVARR417 pKa = 11.84DD418 pKa = 3.28DD419 pKa = 3.56APRR422 pKa = 5.18

Molecular weight: 46.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2766

0

2766

1014669

31

5749

366.8

40.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.707 ± 0.052

0.786 ± 0.015

5.795 ± 0.032

6.482 ± 0.045

3.862 ± 0.029

7.788 ± 0.049

2.308 ± 0.02

5.931 ± 0.042

2.638 ± 0.041

10.551 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.32 ± 0.02

2.856 ± 0.03

4.887 ± 0.037

4.533 ± 0.034

7.197 ± 0.042

6.054 ± 0.027

5.166 ± 0.032

6.92 ± 0.033

1.192 ± 0.019

3.027 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski