Vibrio phage RYC

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 261 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E1GDI5|A0A1E1GDI5_9CAUD Uncharacterized protein OS=Vibrio phage RYC OX=1653734 PE=4 SV=1
MM1 pKa = 7.62DD2 pKa = 4.64TDD4 pKa = 3.47NGKK7 pKa = 7.46WTSSHH12 pKa = 5.77MSLLFTQDD20 pKa = 3.61EE21 pKa = 4.63IEE23 pKa = 4.6GPHH26 pKa = 6.35MYY28 pKa = 10.62NPLNGTQFSPPVVKK42 pKa = 10.28FVEE45 pKa = 4.46PVDD48 pKa = 4.89DD49 pKa = 5.98FINSMLSFNRR59 pKa = 11.84NQPYY63 pKa = 10.32SRR65 pKa = 11.84EE66 pKa = 3.75EE67 pKa = 3.72WLLSDD72 pKa = 5.46DD73 pKa = 4.93EE74 pKa = 4.91LFEE77 pKa = 4.25EE78 pKa = 4.09QGFYY82 pKa = 11.02NGLLGVYY89 pKa = 9.39IDD91 pKa = 4.66CDD93 pKa = 3.97SMTEE97 pKa = 4.25KK98 pKa = 10.73VNQYY102 pKa = 10.19LDD104 pKa = 4.08DD105 pKa = 3.92MMGEE109 pKa = 4.17

Molecular weight: 12.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E1GDC2|A0A1E1GDC2_9CAUD Uncharacterized protein OS=Vibrio phage RYC OX=1653734 PE=4 SV=1
MM1 pKa = 7.64FKK3 pKa = 10.65GQVIRR8 pKa = 11.84IHH10 pKa = 6.61GKK12 pKa = 7.58QVPLIRR18 pKa = 11.84TVRR21 pKa = 11.84NVRR24 pKa = 11.84NFLDD28 pKa = 3.78GGSAVKK34 pKa = 10.71GDD36 pKa = 3.54VKK38 pKa = 10.58IGNKK42 pKa = 9.61VYY44 pKa = 9.68TVYY47 pKa = 10.78RR48 pKa = 11.84SNTHH52 pKa = 4.42RR53 pKa = 11.84QEE55 pKa = 3.61WNLYY59 pKa = 9.59NLATT63 pKa = 4.07

Molecular weight: 7.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

261

0

261

47368

33

1162

181.5

20.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.774 ± 0.18

1.341 ± 0.08

6.669 ± 0.149

8.132 ± 0.205

4.362 ± 0.096

6.57 ± 0.172

1.976 ± 0.085

5.926 ± 0.126

6.823 ± 0.261

8.362 ± 0.193

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.548 ± 0.088

5.117 ± 0.146

3.274 ± 0.106

3.471 ± 0.085

4.429 ± 0.109

6.321 ± 0.151

6.143 ± 0.2

7.112 ± 0.132

1.417 ± 0.059

4.233 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski