Maize streak virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|B6CZV2|B6CZV2_9GEMI Replication-associated protein OS=Maize streak virus OX=10821 PE=3 SV=1
MM1 pKa = 7.36TSSSSNRR8 pKa = 11.84QFLHH12 pKa = 6.69RR13 pKa = 11.84NVNTFLTYY21 pKa = 8.18PHH23 pKa = 7.01CPEE26 pKa = 3.84NPEE29 pKa = 4.12IVSQKK34 pKa = 9.79LWEE37 pKa = 4.12LVGRR41 pKa = 11.84WKK43 pKa = 10.25PLYY46 pKa = 9.81IICARR51 pKa = 11.84EE52 pKa = 3.67AHH54 pKa = 6.6EE55 pKa = 5.52DD56 pKa = 3.97GNLHH60 pKa = 6.05LHH62 pKa = 6.22VLIQTDD68 pKa = 3.77KK69 pKa = 10.81PVRR72 pKa = 11.84TSDD75 pKa = 3.81SRR77 pKa = 11.84FFDD80 pKa = 2.91IDD82 pKa = 3.48GFHH85 pKa = 7.27PNIQSAMSVNKK96 pKa = 9.81VRR98 pKa = 11.84DD99 pKa = 3.94YY100 pKa = 11.14ILKK103 pKa = 10.2EE104 pKa = 3.57PLALFEE110 pKa = 5.16RR111 pKa = 11.84GTFIPRR117 pKa = 11.84KK118 pKa = 9.66KK119 pKa = 10.36SVLGNSSKK127 pKa = 11.16GNSEE131 pKa = 4.12KK132 pKa = 10.7KK133 pKa = 9.55PSKK136 pKa = 10.67DD137 pKa = 3.89EE138 pKa = 3.83IMQDD142 pKa = 3.59IISHH146 pKa = 5.63ATSKK150 pKa = 10.82RR151 pKa = 11.84EE152 pKa = 3.77YY153 pKa = 10.24LSMVRR158 pKa = 11.84KK159 pKa = 9.97SFPYY163 pKa = 10.17DD164 pKa = 2.76WATKK168 pKa = 9.61LQYY171 pKa = 10.4FEE173 pKa = 4.89YY174 pKa = 10.06SASKK178 pKa = 10.37LFPDD182 pKa = 3.43IQEE185 pKa = 4.21EE186 pKa = 4.74FINPHH191 pKa = 6.03PTSEE195 pKa = 4.43PDD197 pKa = 3.53LLCNEE202 pKa = 5.12SIKK205 pKa = 11.07DD206 pKa = 3.33WLQPNIYY213 pKa = 9.41QVSPEE218 pKa = 4.47AYY220 pKa = 8.91MLLQPTCYY228 pKa = 10.31SVDD231 pKa = 3.65DD232 pKa = 5.6AISDD236 pKa = 4.18LQWLDD241 pKa = 3.79DD242 pKa = 4.03VSSKK246 pKa = 11.14QIMDD250 pKa = 3.34QGSRR254 pKa = 11.84ASTSSVQQGQGNLLGPEE271 pKa = 3.97AA272 pKa = 4.96

Molecular weight:
31.19 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|B6CZV0|B6CZV0_9GEMI Movement protein OS=Maize streak virus OX=10821 PE=3 SV=1
MM1 pKa = 6.95STSKK5 pKa = 10.63RR6 pKa = 11.84KK7 pKa = 9.69RR8 pKa = 11.84ADD10 pKa = 3.22EE11 pKa = 4.25AQWNQRR17 pKa = 11.84STKK20 pKa = 9.94KK21 pKa = 10.25KK22 pKa = 10.28ASAPKK27 pKa = 9.94AKK29 pKa = 10.2KK30 pKa = 10.35AGGKK34 pKa = 8.85GEE36 pKa = 4.18KK37 pKa = 9.62PSLQIQTLLHH47 pKa = 6.55SGDD50 pKa = 3.49TMITVPSGGVCDD62 pKa = 6.17LINTYY67 pKa = 11.02ARR69 pKa = 11.84GSDD72 pKa = 3.04EE73 pKa = 4.39GNRR76 pKa = 11.84HH77 pKa = 4.54TSEE80 pKa = 3.79TLTYY84 pKa = 10.21KK85 pKa = 10.8VGVDD89 pKa = 3.04YY90 pKa = 11.43HH91 pKa = 6.46FVADD95 pKa = 4.09AASCKK100 pKa = 9.91YY101 pKa = 10.2SNRR104 pKa = 11.84GTGVMWLVYY113 pKa = 9.39DD114 pKa = 4.05TTPGGNAPTTKK125 pKa = 10.51DD126 pKa = 2.52IFAYY130 pKa = 10.59SDD132 pKa = 3.75ALKK135 pKa = 10.5AWPTTWKK142 pKa = 10.31VSRR145 pKa = 11.84EE146 pKa = 3.85LCHH149 pKa = 6.49RR150 pKa = 11.84FVVKK154 pKa = 10.41RR155 pKa = 11.84RR156 pKa = 11.84WLFTMEE162 pKa = 3.72TDD164 pKa = 3.84GRR166 pKa = 11.84IGSDD170 pKa = 3.46TPPSNASWPPCKK182 pKa = 10.18RR183 pKa = 11.84NVDD186 pKa = 4.1FHH188 pKa = 7.43KK189 pKa = 9.79FTSGLGVRR197 pKa = 11.84TQWKK201 pKa = 8.76NVTDD205 pKa = 4.02GGVGAIQRR213 pKa = 11.84GALYY217 pKa = 10.5LVIAPGNGITFTAHH231 pKa = 5.19GQTRR235 pKa = 11.84LYY237 pKa = 9.73FKK239 pKa = 10.92SVGNQQ244 pKa = 2.8

Molecular weight:
26.81 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

617

101

272

205.7

23.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.159 ± 1.294

1.459 ± 0.139

5.511 ± 0.833

4.538 ± 1.189

3.566 ± 0.298

6.969 ± 2.09

2.593 ± 0.234

5.186 ± 1.031

6.159 ± 1.541

7.942 ± 1.516

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.783 ± 0.29

4.052 ± 0.626

6.483 ± 1.37

4.214 ± 1.051

5.511 ± 0.688

8.914 ± 1.542

6.969 ± 1.945

6.159 ± 0.971

2.431 ± 0.363

3.404 ± 0.178

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski