Smittium simulii

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Zoopagomycota; Kickxellomycotina; Harpellomycetes; Harpellales; Legeriomycetaceae; Smittium

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6456 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T9YJ32|A0A2T9YJ32_9FUNG Uncharacterized protein OS=Smittium simulii OX=133385 GN=BB561_003876 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84KK3 pKa = 9.62LFFLSLISLLFILDD17 pKa = 3.86TKK19 pKa = 11.47ADD21 pKa = 4.28DD22 pKa = 4.04NTGADD27 pKa = 3.67ANSGEE32 pKa = 4.31NNASNTEE39 pKa = 3.93EE40 pKa = 4.42VIDD43 pKa = 4.47VPLDD47 pKa = 3.49YY48 pKa = 10.8FKK50 pKa = 11.66NNSEE54 pKa = 4.1PVEE57 pKa = 4.05EE58 pKa = 4.62NYY60 pKa = 10.68SDD62 pKa = 5.51EE63 pKa = 4.19YY64 pKa = 10.09LQCVKK69 pKa = 11.01DD70 pKa = 3.73NGVGDD75 pKa = 3.87VSSGEE80 pKa = 3.85FDD82 pKa = 3.17TDD84 pKa = 3.98EE85 pKa = 5.17NILTCRR91 pKa = 11.84CAPDD95 pKa = 4.68GNFSCPDD102 pKa = 3.09NTEE105 pKa = 3.96EE106 pKa = 4.31YY107 pKa = 10.74GFTKK111 pKa = 10.38SVTGNEE117 pKa = 4.21DD118 pKa = 3.08VTDD121 pKa = 3.91YY122 pKa = 10.03LTKK125 pKa = 10.41CRR127 pKa = 11.84NIFNRR132 pKa = 11.84DD133 pKa = 2.96GNPFRR138 pKa = 11.84IYY140 pKa = 10.83DD141 pKa = 3.77GLCFCTEE148 pKa = 3.79TRR150 pKa = 11.84SFQCYY155 pKa = 8.03SRR157 pKa = 11.84YY158 pKa = 10.05HH159 pKa = 6.69GIDD162 pKa = 2.97NLGIDD167 pKa = 3.74SHH169 pKa = 7.64DD170 pKa = 3.44VGVPTFEE177 pKa = 6.63DD178 pKa = 2.67IWNDD182 pKa = 3.39LNSSAEE188 pKa = 4.05EE189 pKa = 3.9

Molecular weight: 21.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T9Y436|A0A2T9Y436_9FUNG CCHC-type domain-containing protein OS=Smittium simulii OX=133385 GN=BB561_006451 PE=4 SV=1
MM1 pKa = 7.38CSKK4 pKa = 10.85RR5 pKa = 11.84ILTIGMLLEE14 pKa = 4.01ITTITTRR21 pKa = 11.84LLGTVLGGEE30 pKa = 4.96LIISTGGMCQLKK42 pKa = 10.35SRR44 pKa = 11.84TPAVSNVLSIVHH56 pKa = 5.61YY57 pKa = 9.09MSAIFTPHH65 pKa = 7.28AIILNRR71 pKa = 11.84IMGAPSFWAITLMHH85 pKa = 7.14RR86 pKa = 11.84DD87 pKa = 3.26STGSQKK93 pKa = 11.26NMFEE97 pKa = 3.91FFIFNRR103 pKa = 11.84LHH105 pKa = 7.44RR106 pKa = 11.84INTT109 pKa = 3.69

Molecular weight: 12.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6456

0

6456

2976403

49

7291

461.0

52.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.367 ± 0.028

1.335 ± 0.012

5.469 ± 0.023

5.677 ± 0.029

4.466 ± 0.022

4.244 ± 0.03

1.874 ± 0.011

7.606 ± 0.023

8.127 ± 0.035

9.317 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.828 ± 0.01

7.829 ± 0.043

4.036 ± 0.023

4.008 ± 0.02

3.552 ± 0.022

10.042 ± 0.036

5.882 ± 0.022

4.904 ± 0.023

0.819 ± 0.008

3.616 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski