Larkinella punicea

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Larkinella

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6294 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A368JVB2|A0A368JVB2_9BACT AraC family transcriptional regulator OS=Larkinella punicea OX=2315727 GN=DUE52_06505 PE=4 SV=1
MM1 pKa = 7.43SLLAVMGMLVSIPPAYY17 pKa = 10.05AQSCYY22 pKa = 11.15APISGTAVQVGGVISATVCAGPLCLGTGTYY52 pKa = 10.75NEE54 pKa = 4.28LQKK57 pKa = 9.83ITDD60 pKa = 3.47NSLEE64 pKa = 4.04TFANINTTIAAQIAQGISVKK84 pKa = 9.38RR85 pKa = 11.84TNGTYY90 pKa = 9.57TGEE93 pKa = 4.11NVVGFILSRR102 pKa = 11.84SALVDD107 pKa = 3.18VSVQSSVTIRR117 pKa = 11.84TYY119 pKa = 11.5LNGNPVSAAQPLNALVSGSALGSVEE144 pKa = 4.33KK145 pKa = 10.13MYY147 pKa = 10.11ATFTTNQEE155 pKa = 3.73FDD157 pKa = 3.54EE158 pKa = 4.42VRR160 pKa = 11.84LNTSLLSAEE169 pKa = 4.28VFSNLRR175 pKa = 11.84IFAAVAFPTSCSTPVSTTACNDD197 pKa = 3.7YY198 pKa = 10.9ISGLGTDD205 pKa = 3.15ATYY208 pKa = 10.87YY209 pKa = 11.22GSVTCAACSLTDD221 pKa = 3.65RR222 pKa = 11.84ANLIDD227 pKa = 4.52GNNANYY233 pKa = 10.61AVLTPTAGVASEE245 pKa = 4.34ISVSVIDD252 pKa = 3.67TKK254 pKa = 11.13KK255 pKa = 10.29KK256 pKa = 10.12YY257 pKa = 9.94KK258 pKa = 10.7AGDD261 pKa = 3.22RR262 pKa = 11.84AGFVIAKK269 pKa = 8.84NQVNALLALNALSTITIEE287 pKa = 4.22TYY289 pKa = 11.0LFGTLQEE296 pKa = 4.56AKK298 pKa = 10.73SPGDD302 pKa = 3.35PTNVVGLSLLAGSNTIPKK320 pKa = 9.59QKK322 pKa = 10.65LGFTTTLDD330 pKa = 3.62FNEE333 pKa = 3.4VRR335 pKa = 11.84IRR337 pKa = 11.84INSLATANVGAIRR350 pKa = 11.84LYY352 pKa = 10.77GAFVEE357 pKa = 5.2ATTCTNCEE365 pKa = 4.02TLLNNAGTGKK375 pKa = 10.56YY376 pKa = 9.92NGAIVTGFQSLGLPWTGVHH395 pKa = 6.68GLGLHH400 pKa = 6.89ALTNSGNAVTSTPTDD415 pKa = 3.28DD416 pKa = 3.45FATYY420 pKa = 9.18TSVVGVLGAGLQFTVEE436 pKa = 3.85NDD438 pKa = 3.54GTDD441 pKa = 3.86FTANTTFAGFHH452 pKa = 5.86ISKK455 pKa = 8.79TGSLIALGLLDD466 pKa = 5.87AITIKK471 pKa = 10.69VYY473 pKa = 11.1NGTTLVDD480 pKa = 3.88EE481 pKa = 4.57STSASLLGASLISGTTTRR499 pKa = 11.84SVVGFYY505 pKa = 10.49PDD507 pKa = 3.14AAFDD511 pKa = 5.01RR512 pKa = 11.84IQLIVNEE519 pKa = 4.63GLLSADD525 pKa = 3.52LAGDD529 pKa = 3.55YY530 pKa = 10.72LIYY533 pKa = 10.56DD534 pKa = 3.95AFVIEE539 pKa = 4.97DD540 pKa = 3.76ADD542 pKa = 4.03GDD544 pKa = 4.42GVPDD548 pKa = 4.06CQDD551 pKa = 2.81VCGTGTGNDD560 pKa = 3.95LLDD563 pKa = 4.11FDD565 pKa = 5.76GDD567 pKa = 4.77GIPDD571 pKa = 3.71TCDD574 pKa = 3.65PDD576 pKa = 5.03DD577 pKa = 5.85DD578 pKa = 5.14NDD580 pKa = 5.16GIPDD584 pKa = 3.71TVEE587 pKa = 3.94NPEE590 pKa = 4.14GDD592 pKa = 3.82PNRR595 pKa = 11.84DD596 pKa = 2.98TDD598 pKa = 4.06GDD600 pKa = 4.07SSPDD604 pKa = 3.92LRR606 pKa = 11.84DD607 pKa = 3.5LDD609 pKa = 4.27SDD611 pKa = 3.78NDD613 pKa = 4.74GIPDD617 pKa = 3.89SVEE620 pKa = 3.88KK621 pKa = 11.09GPDD624 pKa = 3.25GNNPSNTDD632 pKa = 3.06GTDD635 pKa = 3.15VPDD638 pKa = 3.92YY639 pKa = 10.81RR640 pKa = 11.84DD641 pKa = 3.8PDD643 pKa = 3.78SDD645 pKa = 3.91NDD647 pKa = 4.73GIPDD651 pKa = 3.68SVEE654 pKa = 3.76AGPNGDD660 pKa = 3.82EE661 pKa = 4.61PRR663 pKa = 11.84NTDD666 pKa = 3.11GDD668 pKa = 4.27ALPDD672 pKa = 3.9YY673 pKa = 11.2LDD675 pKa = 5.06LDD677 pKa = 4.06SDD679 pKa = 3.94NDD681 pKa = 4.73GILDD685 pKa = 3.95TVEE688 pKa = 4.63KK689 pKa = 10.8GSDD692 pKa = 3.41GNNPTNSDD700 pKa = 3.85TNTGSNPDD708 pKa = 3.62GRR710 pKa = 11.84PDD712 pKa = 4.83YY713 pKa = 11.01IDD715 pKa = 5.67LDD717 pKa = 4.07ADD719 pKa = 3.53NDD721 pKa = 4.74GINDD725 pKa = 3.8VIEE728 pKa = 4.53ANPNGSPTKK737 pKa = 10.36DD738 pKa = 3.4SNGDD742 pKa = 3.72GIADD746 pKa = 4.11GTPGATGIPASAGSGLTPPDD766 pKa = 3.38TDD768 pKa = 4.54GDD770 pKa = 4.2TKK772 pKa = 11.21PDD774 pKa = 3.64FQDD777 pKa = 3.15IDD779 pKa = 3.73TDD781 pKa = 3.95GDD783 pKa = 4.4GIPDD787 pKa = 4.33LYY789 pKa = 11.09EE790 pKa = 4.74SGIPNPNALDD800 pKa = 3.75TNKK803 pKa = 10.9DD804 pKa = 3.66GIIDD808 pKa = 4.11DD809 pKa = 4.93ASEE812 pKa = 4.1TDD814 pKa = 3.3NDD816 pKa = 5.44GIPTVNAPAAVVDD829 pKa = 4.59GANGTYY835 pKa = 10.88GDD837 pKa = 4.53ANSPALPNNDD847 pKa = 2.17GDD849 pKa = 4.31AYY851 pKa = 10.21PNYY854 pKa = 10.04RR855 pKa = 11.84DD856 pKa = 4.59LDD858 pKa = 3.84SDD860 pKa = 3.75NDD862 pKa = 4.86GITDD866 pKa = 3.88TVEE869 pKa = 3.92KK870 pKa = 10.57GIDD873 pKa = 3.53GANPTNTDD881 pKa = 2.79GDD883 pKa = 4.53LLPDD887 pKa = 3.94YY888 pKa = 11.18LDD890 pKa = 4.96LDD892 pKa = 4.15SDD894 pKa = 3.93NDD896 pKa = 4.59GINDD900 pKa = 3.5VVEE903 pKa = 4.39ATGSPTNDD911 pKa = 3.15SNGDD915 pKa = 3.84GIADD919 pKa = 4.11GTPGATGIPSSAGSGVTPADD939 pKa = 3.84ADD941 pKa = 3.76NDD943 pKa = 3.75GSPNYY948 pKa = 9.88RR949 pKa = 11.84DD950 pKa = 4.12LDD952 pKa = 3.7SDD954 pKa = 3.83NDD956 pKa = 4.14SKK958 pKa = 11.71KK959 pKa = 10.71DD960 pKa = 3.56LYY962 pKa = 11.19EE963 pKa = 4.8SGIPNPASLDD973 pKa = 3.76TNGDD977 pKa = 3.84GVVDD981 pKa = 4.17AADD984 pKa = 3.77GTDD987 pKa = 3.45NDD989 pKa = 5.84GIPQTVDD996 pKa = 3.29GEE998 pKa = 4.37PNAYY1002 pKa = 9.73GDD1004 pKa = 4.13SGNPTLPNEE1013 pKa = 4.6DD1014 pKa = 3.23TDD1016 pKa = 4.08EE1017 pKa = 4.65NPDD1020 pKa = 3.91YY1021 pKa = 10.73IDD1023 pKa = 3.81PAGVDD1028 pKa = 4.19LYY1030 pKa = 9.47PTLSMPDD1037 pKa = 3.39RR1038 pKa = 11.84LYY1040 pKa = 11.13GASGGAKK1047 pKa = 8.42NLKK1050 pKa = 8.04VQVYY1054 pKa = 10.17NLIPATNTNGTITLTISRR1072 pKa = 11.84PSPFYY1077 pKa = 11.07TMALSTAEE1085 pKa = 4.07ALQDD1089 pKa = 3.23QWILTTEE1096 pKa = 3.94TLLFKK1101 pKa = 10.63LVSKK1105 pKa = 9.73TGVTIAGSSFNEE1117 pKa = 3.64IEE1119 pKa = 4.27MVLTASGSAAPGLANLNINILAGSGGEE1146 pKa = 4.1TNTANNVASAQITIEE1161 pKa = 3.9

Molecular weight:
119.49 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A368JGN6|A0A368JGN6_9BACT 3-oxoacyl-[acyl-carrier-protein] synthase 3 OS=Larkinella punicea OX=2315727 GN=fabH PE=3 SV=1
MM1 pKa = 7.64PKK3 pKa = 10.07SLSFRR8 pKa = 11.84SGLGSVLFWSVISAAFIGPGTVTTCAMAGSRR39 pKa = 11.84FGLDD43 pKa = 3.69LLWALTFSTIGTIVLQEE60 pKa = 3.5AAARR64 pKa = 11.84ITIASGLSLGQVVARR79 pKa = 11.84SYY81 pKa = 10.1GTGSRR86 pKa = 11.84WIAGALLFSIVLGCAAYY103 pKa = 10.05QAGNILGAVAGLSMILPVSPVWLTAGIGLVCIFLLWQGSTRR144 pKa = 11.84LIANFLGVIVFVMGVAFAYY163 pKa = 10.18VATRR167 pKa = 11.84TDD169 pKa = 2.93VSAGMFVKK177 pKa = 10.74ALVVPVATADD187 pKa = 3.66SFVLVIGLIGTTIVPYY203 pKa = 10.94NLFLASGLGKK213 pKa = 10.22GQSLAEE219 pKa = 4.01MRR221 pKa = 11.84WGIGLAVLIGGLISMAILVTGTLVTGEE248 pKa = 4.75FSFQQVADD256 pKa = 3.84ALANRR261 pKa = 11.84VGGWSRR267 pKa = 11.84RR268 pKa = 11.84LFAFGLFAAGFTSALTAPMAAAVTSQSLLGWAGSSWRR305 pKa = 11.84YY306 pKa = 8.62RR307 pKa = 11.84LVWLGVMGVGLMFGLMNARR326 pKa = 11.84PVPVIIVVQAINGVLLPVVTIFLFRR351 pKa = 11.84AVNDD355 pKa = 3.48KK356 pKa = 11.05KK357 pKa = 10.96LIPEE361 pKa = 4.75AYY363 pKa = 9.81RR364 pKa = 11.84NSGLQNLAMLVVVAVSLLFGGWNVWLAVQNWLKK397 pKa = 11.12

Molecular weight:
41.61 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6294

0

6294

2371370

21

2512

376.8

42.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.941 ± 0.028

0.717 ± 0.009

5.236 ± 0.024

5.51 ± 0.027

4.672 ± 0.021

7.223 ± 0.029

1.815 ± 0.016

5.862 ± 0.026

5.449 ± 0.028

10.058 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.071 ± 0.015

4.731 ± 0.024

4.639 ± 0.022

4.271 ± 0.02

5.3 ± 0.024

6.13 ± 0.024

6.313 ± 0.032

6.913 ± 0.022

1.363 ± 0.011

3.786 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski