Rhizobium sp. BK376

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7471 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V2UB25|A0A4V2UB25_9RHIZ 4 5-dihydroxyphthalate decarboxylase OS=Rhizobium sp. BK376 OX=2512149 GN=EV561_10563 PE=4 SV=1
MM1 pKa = 8.19PIPQVSDD8 pKa = 3.86TIHH11 pKa = 6.87LNHH14 pKa = 6.88SDD16 pKa = 3.99AGDD19 pKa = 3.73GNAGNGGDD27 pKa = 4.48GYY29 pKa = 11.04NHH31 pKa = 7.13GDD33 pKa = 2.86ISYY36 pKa = 10.92NPIAFVANTQTVEE49 pKa = 3.76GAYY52 pKa = 9.52TDD54 pKa = 3.73LHH56 pKa = 7.48NGDD59 pKa = 5.12HH60 pKa = 5.9VWQAAGWGSGDD71 pKa = 3.45GGAGGASQAQNNGFFAAVTSGAGGAGGDD99 pKa = 3.73SHH101 pKa = 9.09SDD103 pKa = 3.23GDD105 pKa = 3.79QGSLSGGDD113 pKa = 3.47TAAVAAATTATQNTQLMADD132 pKa = 3.18QHH134 pKa = 5.39ATILAGVGGNGGNGNLAQGGDD155 pKa = 2.93ISAAVVHH162 pKa = 6.66SDD164 pKa = 4.32PEE166 pKa = 4.42TTTVNNALDD175 pKa = 3.65HH176 pKa = 5.89FVNAFGDD183 pKa = 3.4IDD185 pKa = 4.41LSHH188 pKa = 7.07LGSS191 pKa = 3.71

Molecular weight: 18.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V2U9Y4|A0A4V2U9Y4_9RHIZ Arsenite efflux membrane protein ArsB OS=Rhizobium sp. BK376 OX=2512149 GN=EV561_108112 PE=4 SV=1
MM1 pKa = 7.76LFRR4 pKa = 11.84EE5 pKa = 4.3PEE7 pKa = 3.64IPEE10 pKa = 4.14ASRR13 pKa = 11.84HH14 pKa = 5.31LPAGMEE20 pKa = 3.81ATGRR24 pKa = 11.84FSFDD28 pKa = 2.57EE29 pKa = 3.93SARR32 pKa = 11.84RR33 pKa = 11.84LNIEE37 pKa = 3.47ASVRR41 pKa = 11.84VLDD44 pKa = 4.33AVCGCGGRR52 pKa = 11.84LRR54 pKa = 11.84RR55 pKa = 11.84HH56 pKa = 5.78GRR58 pKa = 11.84RR59 pKa = 11.84QLVIADD65 pKa = 3.42VPYY68 pKa = 10.37RR69 pKa = 11.84GIPVRR74 pKa = 11.84IILVYY79 pKa = 9.83PRR81 pKa = 11.84LRR83 pKa = 11.84CGVCLTSRR91 pKa = 11.84RR92 pKa = 11.84PAIGDD97 pKa = 2.85IDD99 pKa = 3.59HH100 pKa = 7.03RR101 pKa = 11.84HH102 pKa = 4.96RR103 pKa = 11.84MSRR106 pKa = 11.84RR107 pKa = 11.84LLVHH111 pKa = 6.22LWEE114 pKa = 4.61EE115 pKa = 4.02ARR117 pKa = 11.84SRR119 pKa = 11.84PIADD123 pKa = 3.27IAHH126 pKa = 6.55SIGLDD131 pKa = 3.04EE132 pKa = 4.22KK133 pKa = 9.77TVRR136 pKa = 11.84NALASKK142 pKa = 10.26RR143 pKa = 11.84VSEE146 pKa = 4.36LDD148 pKa = 3.31NRR150 pKa = 11.84ICSVPQILGVARR162 pKa = 11.84CHH164 pKa = 5.53IKK166 pKa = 10.88GRR168 pKa = 11.84VTTVFWDD175 pKa = 3.26VGRR178 pKa = 11.84RR179 pKa = 11.84NLVDD183 pKa = 3.19IVFSDD188 pKa = 3.53RR189 pKa = 11.84ASDD192 pKa = 3.47VRR194 pKa = 11.84RR195 pKa = 11.84WLDD198 pKa = 3.27AFPEE202 pKa = 4.28RR203 pKa = 11.84EE204 pKa = 4.28LVSVVAIDD212 pKa = 3.5MVEE215 pKa = 4.01AYY217 pKa = 9.99HH218 pKa = 7.1RR219 pKa = 11.84AVQEE223 pKa = 4.03ALPEE227 pKa = 3.85ARR229 pKa = 11.84IAVHH233 pKa = 6.24PSGLRR238 pKa = 11.84KK239 pKa = 9.66RR240 pKa = 11.84FKK242 pKa = 10.73DD243 pKa = 3.44AADD246 pKa = 3.47KK247 pKa = 10.82VLKK250 pKa = 10.76NSTLTRR256 pKa = 11.84FKK258 pKa = 10.79ATDD261 pKa = 3.81PYY263 pKa = 10.43FAAHH267 pKa = 7.21RR268 pKa = 11.84ALCASGRR275 pKa = 11.84KK276 pKa = 8.45FQMQTLLDD284 pKa = 4.67LNPLMRR290 pKa = 11.84SAMEE294 pKa = 3.73ARR296 pKa = 11.84RR297 pKa = 11.84NYY299 pKa = 9.71HH300 pKa = 6.44DD301 pKa = 3.25VWKK304 pKa = 10.81ARR306 pKa = 11.84TPYY309 pKa = 10.22AAAIRR314 pKa = 11.84LEE316 pKa = 4.1SFLNNLSLLVLRR328 pKa = 11.84SFGDD332 pKa = 3.11IVSVVRR338 pKa = 11.84RR339 pKa = 11.84WARR342 pKa = 11.84QLADD346 pKa = 3.29STGIPGLKK354 pKa = 10.07GYY356 pKa = 9.92GAARR360 pKa = 11.84SMLDD364 pKa = 2.92RR365 pKa = 11.84AVANITAEE373 pKa = 4.12EE374 pKa = 4.43VEE376 pKa = 4.23NGSLGRR382 pKa = 11.84RR383 pKa = 11.84SPFDD387 pKa = 3.57DD388 pKa = 4.07CSIRR392 pKa = 11.84LCQCCDD398 pKa = 3.34HH399 pKa = 6.7EE400 pKa = 4.66NIRR403 pKa = 11.84YY404 pKa = 9.65GGGSRR409 pKa = 11.84LPRR412 pKa = 11.84SARR415 pKa = 11.84FGATILNPPVAKK427 pKa = 10.15RR428 pKa = 11.84FRR430 pKa = 11.84SIRR433 pKa = 11.84WICHH437 pKa = 4.23EE438 pKa = 5.05CNWSDD443 pKa = 4.33AEE445 pKa = 4.22VMLAQRR451 pKa = 11.84PNGLLRR457 pKa = 11.84ARR459 pKa = 4.41

Molecular weight: 51.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7471

0

7471

2212000

29

4713

296.1

32.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.556 ± 0.043

0.816 ± 0.008

5.764 ± 0.023

5.571 ± 0.03

3.926 ± 0.019

8.186 ± 0.028

2.07 ± 0.014

5.864 ± 0.018

3.768 ± 0.025

9.945 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.584 ± 0.012

2.94 ± 0.018

4.878 ± 0.022

3.189 ± 0.018

6.556 ± 0.032

6.094 ± 0.029

5.362 ± 0.023

7.286 ± 0.022

1.295 ± 0.011

2.351 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski