Odontoglossum ringspot virus (isolate Singapore 1) (ORSV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Tobamovirus; Odontoglossum ringspot virus

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q84136|CAPSD_ORSVS Capsid protein OS=Odontoglossum ringspot virus (isolate Singapore 1) OX=138662 GN=CP PE=3 SV=3
MM1 pKa = 7.87SYY3 pKa = 10.61TITDD7 pKa = 3.91PSKK10 pKa = 10.62LAYY13 pKa = 10.2LSSAWADD20 pKa = 3.42PNSLINLCTNSLGNQFQTQQARR42 pKa = 11.84TTVQQQFADD51 pKa = 3.29VWQPVPTLTSRR62 pKa = 11.84FPAGAGYY69 pKa = 9.93FRR71 pKa = 11.84VYY73 pKa = 10.28RR74 pKa = 11.84YY75 pKa = 10.74DD76 pKa = 5.0PILDD80 pKa = 3.81PLITFLMGTFDD91 pKa = 3.16TRR93 pKa = 11.84NRR95 pKa = 11.84IIEE98 pKa = 4.12VEE100 pKa = 4.11NPQNPTTTEE109 pKa = 3.82TLDD112 pKa = 2.98ATRR115 pKa = 11.84RR116 pKa = 11.84VDD118 pKa = 3.5DD119 pKa = 3.56ATVAIRR125 pKa = 11.84SAINNLLNEE134 pKa = 4.65LVRR137 pKa = 11.84GTGMYY142 pKa = 10.06NQVSFEE148 pKa = 4.28TMSGLTWTSSS158 pKa = 2.94

Molecular weight: 17.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q84136|CAPSD_ORSVS Capsid protein OS=Odontoglossum ringspot virus (isolate Singapore 1) OX=138662 GN=CP PE=3 SV=3
MM1 pKa = 7.13GRR3 pKa = 11.84LRR5 pKa = 11.84FVVLLSIFPIKK16 pKa = 9.79TFSEE20 pKa = 4.67PCSTMALVLRR30 pKa = 11.84DD31 pKa = 3.82SIKK34 pKa = 10.14ISEE37 pKa = 5.09FINLSASEE45 pKa = 4.28KK46 pKa = 10.15LLPSALTAVKK56 pKa = 9.87SVRR59 pKa = 11.84ISKK62 pKa = 9.58VDD64 pKa = 3.69KK65 pKa = 10.57IISYY69 pKa = 10.88EE70 pKa = 3.98NDD72 pKa = 3.31TLSDD76 pKa = 3.3IDD78 pKa = 3.91LLKK81 pKa = 10.73GVKK84 pKa = 9.67LVEE87 pKa = 3.99NGYY90 pKa = 9.81VCLAGLVVTGEE101 pKa = 3.84WNLPDD106 pKa = 3.51NCKK109 pKa = 10.69GGVSICLVDD118 pKa = 4.54KK119 pKa = 10.87RR120 pKa = 11.84MKK122 pKa = 10.14RR123 pKa = 11.84ANEE126 pKa = 3.84ATLGSYY132 pKa = 7.53HH133 pKa = 6.79TSACKK138 pKa = 10.09KK139 pKa = 9.82RR140 pKa = 11.84FTFKK144 pKa = 10.61IIPNYY149 pKa = 10.75SVTTADD155 pKa = 3.35ALKK158 pKa = 10.81GIWQVMINIRR168 pKa = 11.84GVEE171 pKa = 4.04MEE173 pKa = 5.2KK174 pKa = 10.84GFCPLSLEE182 pKa = 4.19FVSICVVYY190 pKa = 10.66LNNIKK195 pKa = 10.6LGLRR199 pKa = 11.84EE200 pKa = 4.4KK201 pKa = 10.37ILNVTEE207 pKa = 4.6GGPTEE212 pKa = 4.04LTEE215 pKa = 4.07AVVDD219 pKa = 3.87EE220 pKa = 4.88FVEE223 pKa = 4.36KK224 pKa = 11.04VPMAARR230 pKa = 11.84LKK232 pKa = 10.71SFRR235 pKa = 11.84SVNKK239 pKa = 9.88KK240 pKa = 10.34KK241 pKa = 10.45PSDD244 pKa = 3.34SSKK247 pKa = 10.51FVNGKK252 pKa = 9.57SRR254 pKa = 11.84LNSRR258 pKa = 11.84NKK260 pKa = 10.32LNYY263 pKa = 9.72EE264 pKa = 4.14NGDD267 pKa = 3.54SDD269 pKa = 4.01VGISVVDD276 pKa = 4.56DD277 pKa = 3.39IVVGNGVSDD286 pKa = 3.3IRR288 pKa = 11.84IDD290 pKa = 4.04DD291 pKa = 4.05DD292 pKa = 4.61CEE294 pKa = 4.88SFDD297 pKa = 4.49AQSDD301 pKa = 4.35PYY303 pKa = 11.45

Molecular weight: 33.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3185

158

1612

796.3

90.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.934 ± 0.255

2.135 ± 0.202

6.499 ± 0.107

5.746 ± 0.329

5.243 ± 0.252

4.898 ± 0.253

1.947 ± 0.384

5.275 ± 0.405

7.567 ± 0.877

9.294 ± 0.089

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.512 ± 0.108

4.584 ± 0.396

4.05 ± 0.242

3.234 ± 0.573

4.584 ± 0.244

7.63 ± 0.39

5.651 ± 0.974

8.289 ± 0.592

0.973 ± 0.126

3.925 ± 0.282
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski