Firmicutes bacterium CAG:194

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2310 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5QP50|R5QP50_9FIRM Transcription-repair-coupling factor OS=Firmicutes bacterium CAG:194 OX=1263008 GN=mfd PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 10.46KK3 pKa = 10.37KK4 pKa = 10.38ILTLMLAACMVTALTACGSSKK25 pKa = 11.15EE26 pKa = 4.2EE27 pKa = 4.28GQSQDD32 pKa = 3.08TTEE35 pKa = 3.86VSGNGIISTKK45 pKa = 10.64DD46 pKa = 3.52YY47 pKa = 11.57DD48 pKa = 3.88PDD50 pKa = 5.49DD51 pKa = 3.88YY52 pKa = 12.03VKK54 pKa = 10.98LGDD57 pKa = 3.84YY58 pKa = 10.88EE59 pKa = 5.22GITVNTDD66 pKa = 2.21VYY68 pKa = 10.07TYY70 pKa = 11.0TDD72 pKa = 3.88ADD74 pKa = 3.03VDD76 pKa = 3.81AQFDD80 pKa = 3.96KK81 pKa = 11.04EE82 pKa = 3.72INYY85 pKa = 9.27YY86 pKa = 9.11VEE88 pKa = 5.09YY89 pKa = 9.88MDD91 pKa = 5.62AYY93 pKa = 10.82DD94 pKa = 3.63YY95 pKa = 9.46TATDD99 pKa = 3.38KK100 pKa = 10.48QTVEE104 pKa = 4.09TGDD107 pKa = 3.84TVNIDD112 pKa = 3.31YY113 pKa = 9.66TGTKK117 pKa = 10.22DD118 pKa = 3.96GVAFDD123 pKa = 4.3GGTAQGAHH131 pKa = 6.2LKK133 pKa = 10.05IGSGRR138 pKa = 11.84FIDD141 pKa = 4.31GFEE144 pKa = 4.89DD145 pKa = 3.92GLIGHH150 pKa = 7.45AVGEE154 pKa = 4.56TVNLDD159 pKa = 3.18LTFPEE164 pKa = 4.77NYY166 pKa = 10.29SNADD170 pKa = 3.43LAGAAVVFEE179 pKa = 4.27VKK181 pKa = 10.18INSIDD186 pKa = 3.6EE187 pKa = 4.53GKK189 pKa = 10.74LPEE192 pKa = 5.2INDD195 pKa = 3.82ALIASLNLGYY205 pKa = 9.07DD206 pKa = 3.19TVDD209 pKa = 2.79ACRR212 pKa = 11.84QNVEE216 pKa = 5.09DD217 pKa = 4.24YY218 pKa = 11.22LKK220 pKa = 10.79NACDD224 pKa = 4.2EE225 pKa = 4.48KK226 pKa = 11.51NSDD229 pKa = 3.65AKK231 pKa = 10.85KK232 pKa = 8.87SAVWNAVYY240 pKa = 8.24ATCEE244 pKa = 4.03VSDD247 pKa = 4.31APQEE251 pKa = 3.97LVDD254 pKa = 5.55DD255 pKa = 4.41IKK257 pKa = 10.57TRR259 pKa = 11.84IAANAQYY266 pKa = 10.59YY267 pKa = 9.37ADD269 pKa = 4.1YY270 pKa = 10.87YY271 pKa = 11.3KK272 pKa = 10.9VDD274 pKa = 3.92LATFITNYY282 pKa = 9.54MGLTEE287 pKa = 4.75EE288 pKa = 4.88EE289 pKa = 4.71YY290 pKa = 10.76EE291 pKa = 4.23KK292 pKa = 10.19QTAEE296 pKa = 4.13SAAEE300 pKa = 3.95SAKK303 pKa = 10.67EE304 pKa = 3.86KK305 pKa = 10.7LAIAAIAKK313 pKa = 9.62KK314 pKa = 10.51AGISLSDD321 pKa = 4.31DD322 pKa = 3.64DD323 pKa = 4.69VKK325 pKa = 11.22AAAEE329 pKa = 4.33TEE331 pKa = 4.21YY332 pKa = 11.33SDD334 pKa = 4.36YY335 pKa = 11.34GYY337 pKa = 11.14DD338 pKa = 3.78SADD341 pKa = 3.48ALLSAIGQGAYY352 pKa = 10.27YY353 pKa = 10.44DD354 pKa = 4.06YY355 pKa = 11.61VLSDD359 pKa = 3.62KK360 pKa = 11.14VNEE363 pKa = 3.97YY364 pKa = 10.95LIDD367 pKa = 3.43QVTIVEE373 pKa = 4.57NDD375 pKa = 4.22PISITEE381 pKa = 4.03TDD383 pKa = 3.21SAAADD388 pKa = 3.6TAEE391 pKa = 4.0EE392 pKa = 4.11DD393 pKa = 4.0VEE395 pKa = 4.91EE396 pKa = 4.14EE397 pKa = 4.1LQEE400 pKa = 4.18EE401 pKa = 4.68EE402 pKa = 4.61SVSGEE407 pKa = 3.97DD408 pKa = 4.65AAVTDD413 pKa = 3.7IAGTEE418 pKa = 3.97IEE420 pKa = 4.78AEE422 pKa = 4.19TEE424 pKa = 4.02EE425 pKa = 4.28

Molecular weight: 46.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5QPK8|R5QPK8_9FIRM 3-oxoacyl-[acyl-carrier-protein] synthase 2 OS=Firmicutes bacterium CAG:194 OX=1263008 GN=BN526_00745 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSAGGRR28 pKa = 11.84KK29 pKa = 8.81VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2310

0

2310

763414

29

2771

330.5

37.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.931 ± 0.052

1.486 ± 0.019

5.764 ± 0.041

7.423 ± 0.066

3.947 ± 0.036

6.911 ± 0.047

1.719 ± 0.023

7.219 ± 0.052

7.007 ± 0.048

9.171 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.145 ± 0.028

4.073 ± 0.033

3.153 ± 0.028

3.694 ± 0.032

4.095 ± 0.036

5.592 ± 0.037

5.651 ± 0.046

6.916 ± 0.038

0.797 ± 0.015

4.306 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski