Pseudomonas sp. 5

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; unclassified Pseudomonas

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4863 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F0E8S3|A0A0F0E8S3_9PSED Uncharacterized protein OS=Pseudomonas sp. 5 OX=1619949 GN=UB47_17935 PE=4 SV=1
MM1 pKa = 7.14TFVVTDD7 pKa = 3.25NCIKK11 pKa = 10.54CKK13 pKa = 10.0YY14 pKa = 7.47TDD16 pKa = 3.85CVEE19 pKa = 4.15VCPVDD24 pKa = 4.13CFYY27 pKa = 11.12EE28 pKa = 4.9GPNFLVIHH36 pKa = 6.99PDD38 pKa = 3.12EE39 pKa = 5.91CIDD42 pKa = 4.16CALCEE47 pKa = 4.25PEE49 pKa = 4.86CPAVAIFSEE58 pKa = 4.74DD59 pKa = 3.48EE60 pKa = 4.34VPSGMEE66 pKa = 4.08NFIEE70 pKa = 4.56LNAEE74 pKa = 4.64LADD77 pKa = 3.08IWPNITEE84 pKa = 5.02KK85 pKa = 10.58KK86 pKa = 10.62DD87 pKa = 3.67SMPDD91 pKa = 3.05AAEE94 pKa = 3.89WDD96 pKa = 3.94GKK98 pKa = 9.36PGKK101 pKa = 10.01IADD104 pKa = 4.01LEE106 pKa = 4.31RR107 pKa = 5.79

Molecular weight: 11.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F0E8C3|A0A0F0E8C3_9PSED Quinolinate synthase A OS=Pseudomonas sp. 5 OX=1619949 GN=nadA PE=3 SV=1
MM1 pKa = 7.42AKK3 pKa = 9.9EE4 pKa = 4.33FPALYY9 pKa = 9.07HH10 pKa = 5.68WWSARR15 pKa = 11.84QDD17 pKa = 3.64RR18 pKa = 11.84TNLEE22 pKa = 4.24PPAHH26 pKa = 6.85IAAQAQAFMGKK37 pKa = 9.55LEE39 pKa = 4.36HH40 pKa = 7.32LLTTRR45 pKa = 11.84QAVCPKK51 pKa = 9.98CGSPDD56 pKa = 3.3MQRR59 pKa = 11.84IDD61 pKa = 3.27QRR63 pKa = 11.84RR64 pKa = 11.84PNFRR68 pKa = 11.84CPGCWTTVSLIKK80 pKa = 10.18GTLLCRR86 pKa = 11.84LGYY89 pKa = 9.35PEE91 pKa = 4.69HH92 pKa = 6.82WLGFAQGLINGEE104 pKa = 4.48SIVDD108 pKa = 3.75LQRR111 pKa = 11.84RR112 pKa = 11.84TGLCGIACKK121 pKa = 10.02RR122 pKa = 11.84WQVRR126 pKa = 11.84FMQMIKK132 pKa = 10.52LQGHH136 pKa = 5.43TEE138 pKa = 4.08LARR141 pKa = 11.84WITWLRR147 pKa = 11.84SRR149 pKa = 11.84RR150 pKa = 11.84VKK152 pKa = 10.29EE153 pKa = 3.62VSDD156 pKa = 4.02FVRR159 pKa = 11.84NGGQLEE165 pKa = 4.23AVTGSRR171 pKa = 11.84YY172 pKa = 9.33SAGSKK177 pKa = 9.94RR178 pKa = 11.84VFNTPPNRR186 pKa = 11.84QRR188 pKa = 11.84KK189 pKa = 8.13

Molecular weight: 21.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4854

9

4863

1611599

29

4320

331.4

36.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.998 ± 0.042

1.035 ± 0.013

5.308 ± 0.028

5.662 ± 0.03

3.618 ± 0.024

7.868 ± 0.035

2.293 ± 0.018

4.712 ± 0.03

3.409 ± 0.032

11.973 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.294 ± 0.017

3.076 ± 0.026

4.764 ± 0.024

4.881 ± 0.035

6.378 ± 0.033

5.854 ± 0.023

4.74 ± 0.029

7.119 ± 0.031

1.445 ± 0.015

2.575 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski