Carnation Italian ringspot virus (CIRV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Tombusvirus

Average proteome isoelectric point is 8.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q66104|P19_CIRV RNA silencing suppressor p19 OS=Carnation Italian ringspot virus OX=39443 GN=ORF4 PE=1 SV=1
MM1 pKa = 7.28EE2 pKa = 5.41RR3 pKa = 11.84AIQGNDD9 pKa = 2.71TRR11 pKa = 11.84EE12 pKa = 3.88QANGEE17 pKa = 4.11RR18 pKa = 11.84WDD20 pKa = 4.14GGSGGITSPFKK31 pKa = 11.15LPDD34 pKa = 3.7EE35 pKa = 4.69SPSWTEE41 pKa = 3.0WRR43 pKa = 11.84LYY45 pKa = 10.85NDD47 pKa = 3.84EE48 pKa = 4.46TNSNQDD54 pKa = 2.84NPLGFKK60 pKa = 10.19EE61 pKa = 3.92SWGFGKK67 pKa = 10.5VVFKK71 pKa = 10.8RR72 pKa = 11.84YY73 pKa = 9.61LRR75 pKa = 11.84YY76 pKa = 10.37DD77 pKa = 3.21RR78 pKa = 11.84TEE80 pKa = 3.87ASLHH84 pKa = 5.75RR85 pKa = 11.84VLGSWTGDD93 pKa = 3.38SVNYY97 pKa = 9.38AASRR101 pKa = 11.84FLGANQVGCTYY112 pKa = 10.56SIRR115 pKa = 11.84FRR117 pKa = 11.84GVSVTISGGSRR128 pKa = 11.84TLQHH132 pKa = 6.31LCEE135 pKa = 3.73MAIRR139 pKa = 11.84SKK141 pKa = 11.07QEE143 pKa = 3.79LLQLTPVEE151 pKa = 4.34VEE153 pKa = 4.42SNVSRR158 pKa = 11.84GCPEE162 pKa = 4.39GIEE165 pKa = 4.3TFKK168 pKa = 11.17KK169 pKa = 10.32EE170 pKa = 4.08SEE172 pKa = 4.1

Molecular weight: 19.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q66103|Q66103_CIRV Movement protein OS=Carnation Italian ringspot virus OX=39443 GN=orf4 PE=3 SV=1
MM1 pKa = 7.25TMVLRR6 pKa = 11.84NSGTMVPQGGNKK18 pKa = 7.67MSKK21 pKa = 7.61MAKK24 pKa = 9.52NASQQGLQAGAAYY37 pKa = 10.82LMSNPKK43 pKa = 10.2GALKK47 pKa = 10.35KK48 pKa = 11.06GKK50 pKa = 9.6DD51 pKa = 2.9AWNYY55 pKa = 4.58MTAGGVPSGIVHH67 pKa = 6.14SGGMKK72 pKa = 9.33GAIMAPVAVTRR83 pKa = 11.84QLRR86 pKa = 11.84GSKK89 pKa = 9.96PKK91 pKa = 10.45FSGRR95 pKa = 11.84TSGSVTVSHH104 pKa = 7.15RR105 pKa = 11.84EE106 pKa = 4.4LITQVNNSTQFVVNGGVTGNLRR128 pKa = 11.84QVNPLNGTLFSWLPSIAANFDD149 pKa = 3.37QYY151 pKa = 11.33TFNSVTLHH159 pKa = 5.31YY160 pKa = 10.97VPLCATTEE168 pKa = 4.03TGRR171 pKa = 11.84VAMYY175 pKa = 10.13FDD177 pKa = 5.07KK178 pKa = 10.94DD179 pKa = 4.05SEE181 pKa = 4.34DD182 pKa = 4.11LEE184 pKa = 4.4PADD187 pKa = 4.12RR188 pKa = 11.84VEE190 pKa = 4.17LANYY194 pKa = 7.65ATLKK198 pKa = 8.78EE199 pKa = 4.05TAPWAEE205 pKa = 3.66AMLRR209 pKa = 11.84IPTDD213 pKa = 3.69RR214 pKa = 11.84IKK216 pKa = 10.68RR217 pKa = 11.84YY218 pKa = 10.24NDD220 pKa = 3.29DD221 pKa = 3.28SSVNDD226 pKa = 3.94RR227 pKa = 11.84KK228 pKa = 10.83LIDD231 pKa = 4.17LGQIGIATYY240 pKa = 10.34GGSGTNPVGDD250 pKa = 3.64VFISYY255 pKa = 10.53SVTLHH260 pKa = 6.42FPQPTAAGVQTRR272 pKa = 11.84RR273 pKa = 11.84LDD275 pKa = 3.56LTSVLDD281 pKa = 4.0TNVGPSYY288 pKa = 10.66TITSSTATVYY298 pKa = 9.64TVAFRR303 pKa = 11.84TVGTFMLFGAVRR315 pKa = 11.84STGSPVIGLSSANIAINSNTTLNTAGTAFSYY346 pKa = 7.1MTNVTVSALPASITFTVVGVITSSTSHH373 pKa = 6.65AVRR376 pKa = 11.84ASRR379 pKa = 11.84TNNADD384 pKa = 3.52MII386 pKa = 4.76

Molecular weight: 40.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1929

172

852

385.8

42.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.776 ± 1.007

1.555 ± 0.382

4.717 ± 0.286

5.547 ± 0.898

3.266 ± 0.266

7.932 ± 0.576

1.763 ± 0.414

4.303 ± 0.238

5.651 ± 0.67

8.917 ± 0.717

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.54 ± 0.277

4.355 ± 0.621

4.458 ± 0.173

2.696 ± 0.346

7.102 ± 0.63

6.843 ± 1.359

6.947 ± 1.18

8.605 ± 0.38

1.348 ± 0.217

3.629 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski