Heterobasidion partitivirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Alphapartitivirus

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E7E1E9|E7E1E9_9VIRU Putative coat protein OS=Heterobasidion partitivirus 1 OX=942041 PE=4 SV=1
MM1 pKa = 8.34DD2 pKa = 4.58YY3 pKa = 10.36LTGLFSRR10 pKa = 11.84VLHH13 pKa = 6.7ISRR16 pKa = 11.84KK17 pKa = 5.46VTNFEE22 pKa = 3.98FAGTYY27 pKa = 9.64HH28 pKa = 5.98YY29 pKa = 10.51QPSIPQVNEE38 pKa = 3.7VATEE42 pKa = 3.8NHH44 pKa = 6.09KK45 pKa = 9.9RR46 pKa = 11.84TLRR49 pKa = 11.84HH50 pKa = 5.27SFRR53 pKa = 11.84TYY55 pKa = 9.43LTSDD59 pKa = 3.65EE60 pKa = 4.02YY61 pKa = 11.8DD62 pKa = 3.88KK63 pKa = 11.21IVNGYY68 pKa = 9.7KK69 pKa = 8.98RR70 pKa = 11.84TNLDD74 pKa = 3.15PSTITEE80 pKa = 4.51DD81 pKa = 3.62FFSGDD86 pKa = 3.46IEE88 pKa = 4.39DD89 pKa = 4.52HH90 pKa = 7.09PEE92 pKa = 3.86PTDD95 pKa = 3.65FKK97 pKa = 10.44SQLSIEE103 pKa = 4.45YY104 pKa = 10.13GLQCMIDD111 pKa = 3.7AFKK114 pKa = 10.53PPAPARR120 pKa = 11.84VCHH123 pKa = 6.74LYY125 pKa = 10.49DD126 pKa = 3.94VQWHH130 pKa = 5.98YY131 pKa = 10.81PFKK134 pKa = 9.3WQVNAEE140 pKa = 4.24APFSTEE146 pKa = 4.86KK147 pKa = 11.1YY148 pKa = 10.6FLDD151 pKa = 3.51LRR153 pKa = 11.84KK154 pKa = 10.25KK155 pKa = 10.72FGDD158 pKa = 3.47FFDD161 pKa = 4.94PVTKK165 pKa = 10.53LWTKK169 pKa = 9.96YY170 pKa = 10.08VNPLDD175 pKa = 3.5ALRR178 pKa = 11.84RR179 pKa = 11.84YY180 pKa = 9.53GHH182 pKa = 6.4TPPADD187 pKa = 3.57TLNQVTPPKK196 pKa = 10.31FGFMKK201 pKa = 10.58NLIFSFVHH209 pKa = 5.54SWQHH213 pKa = 4.84VIKK216 pKa = 10.93SRR218 pKa = 11.84FTSNAGITHH227 pKa = 6.49SNFLRR232 pKa = 11.84QRR234 pKa = 11.84FLFPMLLHH242 pKa = 6.79IKK244 pKa = 8.41TAIVSFDD251 pKa = 3.68APNKK255 pKa = 9.89LRR257 pKa = 11.84SIWGVSKK264 pKa = 10.63LWIISEE270 pKa = 3.97AMIYY274 pKa = 9.27WEE276 pKa = 4.13YY277 pKa = 10.51IAWIKK282 pKa = 10.29LNPGSTPMLWGYY294 pKa = 7.52EE295 pKa = 4.17TFTGGWFRR303 pKa = 11.84LWRR306 pKa = 11.84DD307 pKa = 3.02LHH309 pKa = 6.21TPGEE313 pKa = 4.43DD314 pKa = 2.98VTYY317 pKa = 9.36ITIDD321 pKa = 2.75WSRR324 pKa = 11.84FDD326 pKa = 3.08KK327 pKa = 10.82RR328 pKa = 11.84AYY330 pKa = 9.23FWLIRR335 pKa = 11.84KK336 pKa = 9.19IFIRR340 pKa = 11.84TRR342 pKa = 11.84CFLDD346 pKa = 3.42FTNGYY351 pKa = 10.01VSTKK355 pKa = 10.21DD356 pKa = 3.7YY357 pKa = 7.47PTSPTDD363 pKa = 3.58PDD365 pKa = 3.34KK366 pKa = 11.52LQALWEE372 pKa = 4.09WTIEE376 pKa = 4.06AFFDD380 pKa = 4.15SPIVLPDD387 pKa = 2.96GSMFKK392 pKa = 10.54RR393 pKa = 11.84LFAGIPSGLFITQLMDD409 pKa = 2.64SWYY412 pKa = 10.28NYY414 pKa = 9.21TMLAAILHH422 pKa = 5.01YY423 pKa = 9.74MGYY426 pKa = 10.49DD427 pKa = 3.37PRR429 pKa = 11.84RR430 pKa = 11.84CIIKK434 pKa = 10.07VQGDD438 pKa = 3.48DD439 pKa = 3.95SIIRR443 pKa = 11.84LYY445 pKa = 10.31IQIPLHH451 pKa = 5.24EE452 pKa = 5.68HH453 pKa = 6.91DD454 pKa = 5.42LFLLRR459 pKa = 11.84MQEE462 pKa = 4.1VSDD465 pKa = 3.89HH466 pKa = 6.52LFGAKK471 pKa = 9.25ISFEE475 pKa = 3.94KK476 pKa = 10.94SEE478 pKa = 4.55LRR480 pKa = 11.84NSLIGSEE487 pKa = 4.05VLSYY491 pKa = 11.31RR492 pKa = 11.84NIQGLPYY499 pKa = 9.77RR500 pKa = 11.84DD501 pKa = 4.35LIKK504 pKa = 10.32MLAQFYY510 pKa = 7.91HH511 pKa = 6.55TKK513 pKa = 10.76AKK515 pKa = 10.74DD516 pKa = 3.4PTPEE520 pKa = 3.31ITMAQAIGFAYY531 pKa = 9.62AACGNDD537 pKa = 3.41FRR539 pKa = 11.84IHH541 pKa = 5.78EE542 pKa = 4.71LLRR545 pKa = 11.84SVYY548 pKa = 10.21DD549 pKa = 3.44YY550 pKa = 11.77YY551 pKa = 11.07KK552 pKa = 11.03AQGFTPNPAGLTVVFGDD569 pKa = 3.79SPDD572 pKa = 3.7RR573 pKa = 11.84PDD575 pKa = 3.59YY576 pKa = 10.69PISLDD581 pKa = 3.65EE582 pKa = 5.37FPSQMDD588 pKa = 3.2VQRR591 pKa = 11.84FFLSTDD597 pKa = 3.05YY598 pKa = 11.41RR599 pKa = 11.84NADD602 pKa = 3.27QEE604 pKa = 4.11NRR606 pKa = 11.84TWPSSHH612 pKa = 7.01FLYY615 pKa = 10.62APCSRR620 pKa = 11.84II621 pKa = 3.44

Molecular weight: 72.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E7E1E9|E7E1E9_9VIRU Putative coat protein OS=Heterobasidion partitivirus 1 OX=942041 PE=4 SV=1
MM1 pKa = 7.22AQNTVSAQVPTMEE14 pKa = 4.92PQPAGPGVAQPQVNLAPPVPDD35 pKa = 4.13PRR37 pKa = 11.84DD38 pKa = 3.45VAANAMMPQVPGPMPSSSGALSTITAALGYY68 pKa = 10.64NRR70 pKa = 11.84FLPMTFGRR78 pKa = 11.84STYY81 pKa = 8.95TPSFILLSLVLSEE94 pKa = 5.37FDD96 pKa = 3.64RR97 pKa = 11.84IMINTHH103 pKa = 6.14RR104 pKa = 11.84FYY106 pKa = 10.18QTSPEE111 pKa = 3.75WHH113 pKa = 7.24PIMSQLYY120 pKa = 8.19YY121 pKa = 10.23GVIFIVHH128 pKa = 6.43ILVARR133 pKa = 11.84RR134 pKa = 11.84TAQVISIDD142 pKa = 3.8EE143 pKa = 4.24NNFLDD148 pKa = 3.49WFEE151 pKa = 4.92TNFPLSSLPIAGPLKK166 pKa = 10.76HH167 pKa = 6.55FFQAITVTCGPSKK180 pKa = 10.82YY181 pKa = 9.97YY182 pKa = 11.19GNIYY186 pKa = 9.32PQLNTDD192 pKa = 3.0WTAAFANYY200 pKa = 9.83YY201 pKa = 9.54MPDD204 pKa = 3.18NAISNNHH211 pKa = 6.49LPPLPIFMDD220 pKa = 3.63MLNDD224 pKa = 4.26LLADD228 pKa = 4.0RR229 pKa = 11.84PGRR232 pKa = 11.84AATATVTPRR241 pKa = 11.84LAFDD245 pKa = 4.03HH246 pKa = 7.0RR247 pKa = 11.84NVDD250 pKa = 2.92HH251 pKa = 6.43WRR253 pKa = 11.84SYY255 pKa = 10.6YY256 pKa = 10.85LPLSHH261 pKa = 6.98TFSAMNNSSAVFQFAGMTSLHH282 pKa = 6.63ALPATAMPNWYY293 pKa = 9.31DD294 pKa = 2.99ASLFFGYY301 pKa = 9.71PNRR304 pKa = 11.84IDD306 pKa = 4.17GNALANISTIGEE318 pKa = 4.14FTRR321 pKa = 11.84LGTAGTQHH329 pKa = 6.19TLWFSRR335 pKa = 11.84TIQVMQRR342 pKa = 11.84HH343 pKa = 5.36AQFAKK348 pKa = 10.55DD349 pKa = 3.69STSLASISTKK359 pKa = 10.75GLGACLPILHH369 pKa = 7.02LSANAEE375 pKa = 3.94LSLPNATNNGQTVRR389 pKa = 11.84NVQAIAPNVTGAAAAAVATSPGRR412 pKa = 11.84PAGFAVARR420 pKa = 11.84PTHH423 pKa = 4.75FTVRR427 pKa = 11.84ATSKK431 pKa = 11.04LEE433 pKa = 3.96SLHH436 pKa = 7.02LLAEE440 pKa = 4.23QFSLLSCINLDD451 pKa = 3.65MTNLAARR458 pKa = 11.84GNAYY462 pKa = 10.08HH463 pKa = 7.47AFTTNAQVRR472 pKa = 11.84HH473 pKa = 5.55GPFWDD478 pKa = 3.65LADD481 pKa = 3.92VKK483 pKa = 11.05SHH485 pKa = 5.82TEE487 pKa = 3.75INVLGQISSFLAGAFHH503 pKa = 7.31VDD505 pKa = 3.25QRR507 pKa = 11.84QTKK510 pKa = 9.06

Molecular weight: 55.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1131

510

621

565.5

64.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.311 ± 2.582

0.796 ± 0.139

5.482 ± 1.179

3.095 ± 0.761

6.72 ± 0.694

4.951 ± 0.23

3.36 ± 0.114

6.012 ± 0.746

3.36 ± 1.335

8.93 ± 0.192

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.653 ± 0.326

4.775 ± 1.139

6.985 ± 0.445

3.89 ± 0.416

5.217 ± 0.475

7.162 ± 0.326

7.25 ± 0.53

4.598 ± 0.468

2.122 ± 0.503

4.332 ± 0.934
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski