Anaerotruncus sp. CAG:390

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Anaerotruncus; environmental samples

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2237 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7EM47|R7EM47_9FIRM DUF58 domain-containing protein OS=Anaerotruncus sp. CAG:390 OX=1262703 GN=BN640_00971 PE=4 SV=1
MM1 pKa = 7.68ALLFSAVTVTAGNEE15 pKa = 4.17EE16 pKa = 3.98IEE18 pKa = 4.47FTVGGIPRR26 pKa = 11.84EE27 pKa = 3.92YY28 pKa = 10.63DD29 pKa = 3.69DD30 pKa = 4.93LVGWTAFDD38 pKa = 4.63DD39 pKa = 4.22FMEE42 pKa = 4.5NVDD45 pKa = 4.42AEE47 pKa = 4.81TEE49 pKa = 4.36VSVHH53 pKa = 6.23AEE55 pKa = 3.79SYY57 pKa = 10.7LYY59 pKa = 10.81GGEE62 pKa = 4.16EE63 pKa = 4.44TVTATPEE70 pKa = 3.72EE71 pKa = 4.36VAYY74 pKa = 11.32LMMRR78 pKa = 11.84MEE80 pKa = 4.55RR81 pKa = 11.84DD82 pKa = 3.27EE83 pKa = 4.84NFLAGHH89 pKa = 7.11CDD91 pKa = 3.49NIEE94 pKa = 4.48AIDD97 pKa = 3.96FSFLRR102 pKa = 11.84PPEE105 pKa = 4.18EE106 pKa = 4.15YY107 pKa = 11.03YY108 pKa = 11.04EE109 pKa = 4.22MKK111 pKa = 10.7LL112 pKa = 3.65

Molecular weight: 12.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7EVR9|R7EVR9_9FIRM Uncharacterized protein OS=Anaerotruncus sp. CAG:390 OX=1262703 GN=BN640_00404 PE=4 SV=1
MM1 pKa = 7.89PISAQRR7 pKa = 11.84TKK9 pKa = 10.93RR10 pKa = 11.84STAFILQRR18 pKa = 11.84VQPKK22 pKa = 10.21NMPQTVFPAVLIPSPVRR39 pKa = 11.84LFGSLTNKK47 pKa = 9.99AAVGFNLPKK56 pKa = 10.21TISPKK61 pKa = 9.51QKK63 pKa = 10.39SIFGSGRR70 pKa = 11.84LKK72 pKa = 10.87NPLRR76 pKa = 11.84RR77 pKa = 11.84WSVAKK82 pKa = 10.15KK83 pKa = 9.97RR84 pKa = 11.84SSRR87 pKa = 11.84RR88 pKa = 11.84QNPRR92 pKa = 11.84VNLQLKK98 pKa = 8.1RR99 pKa = 11.84QRR101 pKa = 11.84VQLRR105 pKa = 11.84PHH107 pKa = 7.3RR108 pKa = 11.84KK109 pKa = 9.13RR110 pKa = 11.84LRR112 pKa = 11.84LPNRR116 pKa = 11.84PPEE119 pKa = 4.17LRR121 pKa = 11.84LKK123 pKa = 10.79RR124 pKa = 11.84MNRR127 pKa = 11.84QRR129 pKa = 11.84KK130 pKa = 7.66SLKK133 pKa = 9.46KK134 pKa = 8.9PHH136 pKa = 6.86RR137 pKa = 11.84LRR139 pKa = 11.84LKK141 pKa = 9.78RR142 pKa = 11.84QGRR145 pKa = 11.84QHH147 pKa = 6.92RR148 pKa = 11.84RR149 pKa = 11.84RR150 pKa = 11.84KK151 pKa = 9.48PPPKK155 pKa = 10.04RR156 pKa = 11.84PLPRR160 pKa = 5.25

Molecular weight: 18.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2237

0

2237

673252

29

1667

301.0

33.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.746 ± 0.071

1.764 ± 0.022

6.202 ± 0.043

6.391 ± 0.044

4.102 ± 0.03

7.901 ± 0.046

1.727 ± 0.023

6.435 ± 0.039

5.376 ± 0.048

8.654 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.656 ± 0.024

3.544 ± 0.032

3.785 ± 0.035

1.994 ± 0.022

5.882 ± 0.057

6.182 ± 0.046

5.669 ± 0.042

7.188 ± 0.034

0.851 ± 0.015

3.943 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski