Rachicladosporium sp. CCFEE 5018

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Cladosporiales; Cladosporiaceae; Rachicladosporium; unclassified Rachicladosporium

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18035 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V8TVT8|A0A1V8TVT8_9PEZI Chromatin modification-related protein EAF6 OS=Rachicladosporium sp. CCFEE 5018 OX=1974281 GN=B0A51_16796 PE=3 SV=1
MM1 pKa = 7.69SDD3 pKa = 3.62DD4 pKa = 3.64NTTIGAGNNILSYY17 pKa = 10.38HH18 pKa = 6.14HH19 pKa = 7.19ASPEE23 pKa = 4.04YY24 pKa = 8.89TATAIDD30 pKa = 3.82QFLAAADD37 pKa = 4.01EE38 pKa = 4.73QYY40 pKa = 11.5LSASSDD46 pKa = 3.06SGASDD51 pKa = 3.09RR52 pKa = 11.84SFPVTPPDD60 pKa = 3.88TQSDD64 pKa = 4.19MYY66 pKa = 11.37EE67 pKa = 4.24DD68 pKa = 3.53INFNEE73 pKa = 4.49FVDD76 pKa = 4.72PVAVDD81 pKa = 3.35ALGNTEE87 pKa = 4.66FDD89 pKa = 3.94FSSGTGGSWRR99 pKa = 11.84ADD101 pKa = 3.76TPPSSPPYY109 pKa = 10.05DD110 pKa = 3.58YY111 pKa = 11.34SSPLDD116 pKa = 3.83ALSPPSDD123 pKa = 4.15CAPMQDD129 pKa = 3.59FEE131 pKa = 5.72LMGGLGGVMQYY142 pKa = 11.0LEE144 pKa = 3.93QPNYY148 pKa = 10.67VGFEE152 pKa = 4.29GILYY156 pKa = 10.03DD157 pKa = 4.49HH158 pKa = 7.18EE159 pKa = 4.31PHH161 pKa = 7.06GDD163 pKa = 3.23VATDD167 pKa = 3.6FVVPAASGNLAEE179 pKa = 5.21EE180 pKa = 4.34PSQYY184 pKa = 11.03VFTQSGKK191 pKa = 9.7GYY193 pKa = 8.43VAEE196 pKa = 4.34VEE198 pKa = 4.28VGSYY202 pKa = 10.25EE203 pKa = 3.99GGRR206 pKa = 11.84EE207 pKa = 3.85SWRR210 pKa = 11.84EE211 pKa = 3.81MQIVSMMGTRR221 pKa = 11.84RR222 pKa = 11.84VFTIGEE228 pKa = 3.99ARR230 pKa = 11.84SGGVEE235 pKa = 4.02DD236 pKa = 4.76MSIEE240 pKa = 4.18LEE242 pKa = 3.83WGGRR246 pKa = 11.84AEE248 pKa = 4.15EE249 pKa = 4.0VRR251 pKa = 11.84EE252 pKa = 4.41VLEE255 pKa = 4.42TWCQGKK261 pKa = 10.17KK262 pKa = 9.98GLWWGIWVEE271 pKa = 4.93DD272 pKa = 4.67DD273 pKa = 5.83LPGQQ277 pKa = 3.7

Molecular weight: 30.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V8TG58|A0A1V8TG58_9PEZI Uncharacterized protein OS=Rachicladosporium sp. CCFEE 5018 OX=1974281 GN=B0A51_17770 PE=4 SV=1
MM1 pKa = 7.78APPTTPPNNNTRR13 pKa = 11.84NVYY16 pKa = 9.62AAQPLNTGNIRR27 pKa = 11.84GTGAEE32 pKa = 3.96QAIADD37 pKa = 3.41IDD39 pKa = 3.83QFRR42 pKa = 11.84GGAIIVQYY50 pKa = 10.28DD51 pKa = 3.66AQPVRR56 pKa = 11.84RR57 pKa = 11.84TAEE60 pKa = 3.85HH61 pKa = 6.7NLRR64 pKa = 11.84CQAVKK69 pKa = 9.79SWRR72 pKa = 11.84ICWRR76 pKa = 11.84TSSTMTSLTVCLRR89 pKa = 11.84ASPSSAMRR97 pKa = 11.84LRR99 pKa = 11.84GPSQHH104 pKa = 6.17WGEE107 pKa = 4.03MSRR110 pKa = 11.84TEE112 pKa = 3.9RR113 pKa = 11.84SSKK116 pKa = 10.71GGEE119 pKa = 3.94KK120 pKa = 10.42

Molecular weight: 13.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18035

0

18035

8397328

50

4868

465.6

51.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.698 ± 0.019

1.097 ± 0.007

5.752 ± 0.013

6.098 ± 0.019

3.36 ± 0.012

7.219 ± 0.018

2.385 ± 0.008

4.442 ± 0.013

4.706 ± 0.017

8.712 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.209 ± 0.007

3.321 ± 0.01

6.061 ± 0.019

4.021 ± 0.014

6.234 ± 0.019

8.112 ± 0.021

6.306 ± 0.018

6.205 ± 0.011

1.393 ± 0.007

2.67 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski