Epizootic hemorrhagic disease virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V9SB81|V9SB81_9REOV Outer capsid protein VP2 OS=Epizootic hemorrhagic disease virus OX=40054 GN=VP2 PE=3 SV=1
MM1 pKa = 7.45DD2 pKa = 5.55TIAARR7 pKa = 11.84ALTVIKK13 pKa = 10.52ACNTLKK19 pKa = 10.36EE20 pKa = 3.91VRR22 pKa = 11.84IVVEE26 pKa = 4.5SNVLEE31 pKa = 4.06ILGIAINRR39 pKa = 11.84YY40 pKa = 9.25NGLTLRR46 pKa = 11.84SVTMRR51 pKa = 11.84PTSQEE56 pKa = 3.34QRR58 pKa = 11.84NEE60 pKa = 3.99MFFMCLDD67 pKa = 3.66MVLAAANLNIGNISPDD83 pKa = 3.79YY84 pKa = 9.87IQNLATIGVLATPEE98 pKa = 3.68IPYY101 pKa = 10.22TMEE104 pKa = 3.9SANEE108 pKa = 3.85IARR111 pKa = 11.84MSGEE115 pKa = 4.11TGTWGPDD122 pKa = 3.13RR123 pKa = 11.84QPFGYY128 pKa = 9.89FLTAAEE134 pKa = 4.56VTQHH138 pKa = 4.89GRR140 pKa = 11.84FRR142 pKa = 11.84LRR144 pKa = 11.84AGQNITAAYY153 pKa = 9.63VSSTLAQVSMNAGARR168 pKa = 11.84GDD170 pKa = 3.65IQALFQNQNDD180 pKa = 4.44PIMIYY185 pKa = 9.16FVWRR189 pKa = 11.84RR190 pKa = 11.84IGTFSSAAGNAQDD203 pKa = 3.88TPQGVTLDD211 pKa = 3.59VGGVNMRR218 pKa = 11.84AGVIVAYY225 pKa = 10.33DD226 pKa = 3.42GQAPVNVNNPGAGPGMIEE244 pKa = 4.09IEE246 pKa = 4.28VIYY249 pKa = 10.36YY250 pKa = 10.66LSLDD254 pKa = 3.53KK255 pKa = 11.32TMTQYY260 pKa = 11.23PSLQAQIFNVYY271 pKa = 9.9SYY273 pKa = 11.5KK274 pKa = 10.76NPLWHH279 pKa = 6.91GLRR282 pKa = 11.84AAILNRR288 pKa = 11.84TTLPNNIPPIYY299 pKa = 9.64PPNDD303 pKa = 3.53RR304 pKa = 11.84EE305 pKa = 4.24NVLLLILLSALADD318 pKa = 3.8AFSVLAPDD326 pKa = 4.47FNLFGVVPIQGPINRR341 pKa = 11.84AVAQNAYY348 pKa = 7.85MM349 pKa = 4.54

Molecular weight: 38.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V9SB52|V9SB52_9REOV Core protein VP7 OS=Epizootic hemorrhagic disease virus OX=40054 GN=VP7 PE=3 SV=1
MM1 pKa = 7.88LSRR4 pKa = 11.84LVPGVEE10 pKa = 4.01TRR12 pKa = 11.84IEE14 pKa = 4.02MKK16 pKa = 10.77ASDD19 pKa = 4.27EE20 pKa = 4.26VSLVPYY26 pKa = 9.71QEE28 pKa = 4.0QVRR31 pKa = 11.84PPSYY35 pKa = 10.68VPSAPIPTAMPKK47 pKa = 9.97VALDD51 pKa = 3.69ILDD54 pKa = 3.94KK55 pKa = 11.5AMSNQTGATMAQKK68 pKa = 10.38VEE70 pKa = 4.11KK71 pKa = 10.19VAYY74 pKa = 9.76ASYY77 pKa = 11.54AEE79 pKa = 4.37AFRR82 pKa = 11.84DD83 pKa = 3.96DD84 pKa = 3.69LRR86 pKa = 11.84LRR88 pKa = 11.84QIKK91 pKa = 9.96KK92 pKa = 9.63HH93 pKa = 5.46VNEE96 pKa = 3.91QVLPKK101 pKa = 9.93MRR103 pKa = 11.84VEE105 pKa = 4.13LTAMKK110 pKa = 10.09RR111 pKa = 11.84RR112 pKa = 11.84RR113 pKa = 11.84AMAHH117 pKa = 6.14MILIIAAVVALITSASTLTSDD138 pKa = 4.25LGVILKK144 pKa = 10.67HH145 pKa = 5.3NTTTEE150 pKa = 4.75AIQTYY155 pKa = 10.06IKK157 pKa = 9.49PFCAAFGIINLAATMVMMFMAKK179 pKa = 9.51NEE181 pKa = 4.26KK182 pKa = 10.04IINQQIDD189 pKa = 3.54HH190 pKa = 5.65TRR192 pKa = 11.84KK193 pKa = 10.24EE194 pKa = 4.06IMKK197 pKa = 9.93KK198 pKa = 10.03DD199 pKa = 3.6AYY201 pKa = 10.75NEE203 pKa = 3.94AVRR206 pKa = 11.84MSVTEE211 pKa = 3.98FSGIPLDD218 pKa = 4.54GFDD221 pKa = 5.09IPPEE225 pKa = 4.01LTRR228 pKa = 5.13

Molecular weight: 25.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

6205

228

1302

620.5

71.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.591 ± 0.553

1.08 ± 0.226

5.882 ± 0.274

7.961 ± 0.511

3.884 ± 0.25

5.915 ± 0.34

2.047 ± 0.275

7.462 ± 0.401

6.269 ± 0.619

8.042 ± 0.359

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.626 ± 0.376

3.916 ± 0.308

3.981 ± 0.295

4.11 ± 0.32

7.107 ± 0.261

5.222 ± 0.377

4.996 ± 0.348

6.833 ± 0.225

1.193 ± 0.235

3.884 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski