Melghirimyces thermohalophilus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Thermoactinomycetaceae; Melghirimyces

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G6JPT5|A0A1G6JPT5_9BACL Stage IV sporulation protein FB OS=Melghirimyces thermohalophilus OX=1236220 GN=SAMN04488112_104108 PE=3 SV=1
MM1 pKa = 8.16DD2 pKa = 4.41CQKK5 pKa = 11.1EE6 pKa = 4.16DD7 pKa = 4.53FISEE11 pKa = 4.03DD12 pKa = 3.57VEE14 pKa = 4.27NLEE17 pKa = 4.12EE18 pKa = 4.59PEE20 pKa = 4.69LYY22 pKa = 10.83GILIGLGLWGATLISYY38 pKa = 9.94IVLVRR43 pKa = 11.84LNWIAGLL50 pKa = 3.77

Molecular weight: 5.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G6LV74|A0A1G6LV74_9BACL Aminotransferase OS=Melghirimyces thermohalophilus OX=1236220 GN=SAMN04488112_108141 PE=3 SV=1
MM1 pKa = 7.2VAGTLAFLTCLSMAVLIQSGWFAGLVRR28 pKa = 11.84DD29 pKa = 3.7VGVRR33 pKa = 11.84KK34 pKa = 9.23EE35 pKa = 3.91WLAVGLLLEE44 pKa = 4.44ALLLRR49 pKa = 11.84VQVPPGGMIRR59 pKa = 11.84LQVGMFLFIPFLFCLRR75 pKa = 11.84KK76 pKa = 10.06GNRR79 pKa = 11.84EE80 pKa = 3.72DD81 pKa = 4.05RR82 pKa = 11.84SFLFSSVFFIGSSLFLLRR100 pKa = 11.84EE101 pKa = 4.29LYY103 pKa = 9.5WLEE106 pKa = 4.13PAFSWGISRR115 pKa = 11.84SLEE118 pKa = 3.79MMIVLIIALVSSRR131 pKa = 11.84LLTGRR136 pKa = 11.84LLVASLGMWLGQFLVLCLHH155 pKa = 5.73RR156 pKa = 11.84HH157 pKa = 4.58EE158 pKa = 4.94MKK160 pKa = 10.4PLLFGDD166 pKa = 3.79VQFLDD171 pKa = 4.61FFWLSLILLWVAHH184 pKa = 6.52AVLQQGIQWVRR195 pKa = 11.84GLRR198 pKa = 11.84RR199 pKa = 11.84LII201 pKa = 3.96

Molecular weight: 22.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3229

0

3229

911684

39

1432

282.3

31.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.006 ± 0.046

0.761 ± 0.013

5.134 ± 0.035

7.402 ± 0.055

3.85 ± 0.034

8.013 ± 0.035

2.289 ± 0.021

5.825 ± 0.038

4.986 ± 0.042

10.24 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.761 ± 0.021

2.9 ± 0.023

4.647 ± 0.03

4.087 ± 0.032

6.496 ± 0.048

5.471 ± 0.031

5.138 ± 0.025

7.675 ± 0.039

1.415 ± 0.021

2.904 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski