Enterobacteria phage mEp021

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346FE98|A0A346FE98_9CAUD Putative antirepressor protein OS=Enterobacteria phage mEp021 OX=1150757 GN=mEp021_71 PE=4 SV=1
MM1 pKa = 7.39YY2 pKa = 7.69GTCVVDD8 pKa = 3.31KK9 pKa = 10.36TDD11 pKa = 4.25AFTLFDD17 pKa = 4.89DD18 pKa = 5.13YY19 pKa = 11.33EE20 pKa = 5.58INDD23 pKa = 3.97LTVKK27 pKa = 10.64ADD29 pKa = 5.19NGDD32 pKa = 2.78TWYY35 pKa = 10.93LHH37 pKa = 7.13DD38 pKa = 4.93VGDD41 pKa = 4.54GYY43 pKa = 11.35VGCRR47 pKa = 11.84SRR49 pKa = 11.84EE50 pKa = 3.99GKK52 pKa = 9.85EE53 pKa = 3.67VLFLVDD59 pKa = 4.41GVV61 pKa = 3.71

Molecular weight: 6.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346FE35|A0A346FE35_9CAUD Uncharacterized protein OS=Enterobacteria phage mEp021 OX=1150757 GN=mEp021_08 PE=4 SV=1
MM1 pKa = 7.5RR2 pKa = 11.84RR3 pKa = 11.84VIFYY7 pKa = 10.61SVEE10 pKa = 3.95TFIDD14 pKa = 3.72EE15 pKa = 4.26SHH17 pKa = 7.28FKK19 pKa = 8.93FTCDD23 pKa = 2.94PCGTMVYY30 pKa = 9.14TPPLMRR36 pKa = 11.84KK37 pKa = 8.38YY38 pKa = 10.8KK39 pKa = 9.73HH40 pKa = 6.25VKK42 pKa = 9.09FNRR45 pKa = 11.84AFVPIHH51 pKa = 6.09YY52 pKa = 10.14ALQALRR58 pKa = 11.84GEE60 pKa = 4.76LRR62 pKa = 11.84NTMRR66 pKa = 11.84IVV68 pKa = 2.91

Molecular weight: 8.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

15962

37

1727

194.7

21.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.714 ± 0.379

1.717 ± 0.244

6.422 ± 0.271

6.622 ± 0.271

3.471 ± 0.223

7.092 ± 0.324

1.854 ± 0.175

6.221 ± 0.243

6.622 ± 0.279

7.311 ± 0.302

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.838 ± 0.222

5.313 ± 0.25

3.558 ± 0.242

3.151 ± 0.274

5.776 ± 0.293

5.739 ± 0.391

5.714 ± 0.488

6.797 ± 0.253

1.504 ± 0.113

3.565 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski