Methylopila sp. Yamaguchi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylocystaceae; Methylopila; unclassified Methylopila

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4120 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H6BDX8|A0A2H6BDX8_9RHIZ NUDIX hydrolase OS=Methylopila sp. Yamaguchi OX=1437817 GN=METY_1595 PE=3 SV=1
MM1 pKa = 6.92ITINAKK7 pKa = 10.24SAVFDD12 pKa = 3.3ATGIDD17 pKa = 3.51FEE19 pKa = 5.21AYY21 pKa = 10.11VRR23 pKa = 11.84GQFVADD29 pKa = 3.5ATGSGFPVFDD39 pKa = 3.76NSSAFSGEE47 pKa = 3.86EE48 pKa = 3.52MFIGYY53 pKa = 7.89GTEE56 pKa = 3.89ATSKK60 pKa = 10.48YY61 pKa = 10.22VLAHH65 pKa = 5.58GQVEE69 pKa = 4.79YY70 pKa = 11.25AFGTHH75 pKa = 5.33TVAGTINTIEE85 pKa = 4.24YY86 pKa = 8.19GTIGEE91 pKa = 4.44GSYY94 pKa = 10.8DD95 pKa = 3.47ANGYY99 pKa = 7.32FTGGNVALTITGLDD113 pKa = 3.52LSNPVPADD121 pKa = 3.15NAEE124 pKa = 4.09AVGIEE129 pKa = 4.11ATGAVHH135 pKa = 6.82NFALAHH141 pKa = 5.99MYY143 pKa = 10.49GSSGDD148 pKa = 3.39QARR151 pKa = 11.84FDD153 pKa = 4.11TYY155 pKa = 11.81ADD157 pKa = 3.83ALDD160 pKa = 4.82SEE162 pKa = 4.82AQTFLGSRR170 pKa = 11.84GNDD173 pKa = 3.22VYY175 pKa = 11.61VGTAFNDD182 pKa = 3.93VIRR185 pKa = 11.84SNAGDD190 pKa = 3.65DD191 pKa = 4.03RR192 pKa = 11.84ITGGGGDD199 pKa = 3.7DD200 pKa = 4.28KK201 pKa = 11.17IYY203 pKa = 10.78GSRR206 pKa = 11.84GVDD209 pKa = 3.12TAVYY213 pKa = 10.11SGDD216 pKa = 3.46RR217 pKa = 11.84ADD219 pKa = 3.77YY220 pKa = 10.94AITRR224 pKa = 11.84DD225 pKa = 3.59LAGVLHH231 pKa = 6.24VVDD234 pKa = 5.6LRR236 pKa = 11.84AGATSDD242 pKa = 4.53GADD245 pKa = 2.93QLVDD249 pKa = 3.54VEE251 pKa = 4.37QLEE254 pKa = 4.56FADD257 pKa = 4.28GTLDD261 pKa = 3.55ASTIAAGGMLTLDD274 pKa = 3.62ASGASTGVDD283 pKa = 3.12MTTFLADD290 pKa = 3.9FFAGVATGAAYY301 pKa = 9.56KK302 pKa = 10.3FYY304 pKa = 11.08GGTPDD309 pKa = 3.15QAFGGTYY316 pKa = 10.46YY317 pKa = 10.73MNGDD321 pKa = 3.41QLAYY325 pKa = 10.13QYY327 pKa = 10.89TEE329 pKa = 4.27GGVATDD335 pKa = 3.15ARR337 pKa = 11.84LVFGGEE343 pKa = 3.59EE344 pKa = 3.49LAYY347 pKa = 10.6DD348 pKa = 5.12FIHH351 pKa = 6.97HH352 pKa = 6.79GSQYY356 pKa = 10.54GHH358 pKa = 7.52GITGALDD365 pKa = 3.48SLTFGAWTADD375 pKa = 3.41TTGTEE380 pKa = 4.66GVGSAGLIQNLYY392 pKa = 9.49EE393 pKa = 4.08ALKK396 pKa = 9.29ITGLGFDD403 pKa = 3.91VAPGAGSSNPVHH415 pKa = 6.8LLHH418 pKa = 6.81AAARR422 pKa = 11.84AGDD425 pKa = 3.51ASVFEE430 pKa = 5.18DD431 pKa = 4.29LLASRR436 pKa = 11.84PQHH439 pKa = 6.57FIGSDD444 pKa = 3.36GDD446 pKa = 3.81DD447 pKa = 3.74VYY449 pKa = 11.64VGSAFADD456 pKa = 3.87VVEE459 pKa = 5.22GGAGADD465 pKa = 3.89TLDD468 pKa = 4.11GGQGADD474 pKa = 3.56TLSGGLGDD482 pKa = 3.71DD483 pKa = 3.83TYY485 pKa = 11.92YY486 pKa = 11.15VDD488 pKa = 3.59NSRR491 pKa = 11.84DD492 pKa = 3.73TVIEE496 pKa = 3.93AAGEE500 pKa = 4.14GADD503 pKa = 4.15TIVASADD510 pKa = 3.47FKK512 pKa = 11.28LGARR516 pKa = 11.84VAIEE520 pKa = 3.78TLIAADD526 pKa = 4.8GSDD529 pKa = 4.3ADD531 pKa = 4.07LTANRR536 pKa = 11.84YY537 pKa = 7.7VRR539 pKa = 11.84NLIGSDD545 pKa = 2.92GDD547 pKa = 3.57NRR549 pKa = 11.84LDD551 pKa = 3.45AFAGAKK557 pKa = 8.61TVTGGAGEE565 pKa = 4.21DD566 pKa = 3.53TFVFTVDD573 pKa = 3.16VRR575 pKa = 11.84KK576 pKa = 9.73SAAVISDD583 pKa = 3.76FTSGEE588 pKa = 3.93DD589 pKa = 4.25SIEE592 pKa = 4.11LSSKK596 pKa = 10.21VFTALGVGALDD607 pKa = 3.75AGSFVLGSAALDD619 pKa = 3.89ADD621 pKa = 4.01DD622 pKa = 6.06HH623 pKa = 7.14ILYY626 pKa = 10.06DD627 pKa = 3.96ASTGSLFYY635 pKa = 11.01DD636 pKa = 3.6ADD638 pKa = 4.09GVGGSAALLFATLSSAPSLSAADD661 pKa = 3.83FLVAA665 pKa = 5.42

Molecular weight: 67.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H6BJM6|A0A2H6BJM6_9RHIZ Molybdopterin dehydrogenase FAD-binding OS=Methylopila sp. Yamaguchi OX=1437817 GN=METY_3600 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.31VIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.89GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4120

0

4120

1302561

26

2604

316.2

33.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.658 ± 0.064

0.714 ± 0.011

5.817 ± 0.035

5.695 ± 0.038

3.601 ± 0.025

8.976 ± 0.035

1.786 ± 0.019

4.317 ± 0.028

2.975 ± 0.03

10.212 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.092 ± 0.016

2.092 ± 0.021

5.467 ± 0.029

2.519 ± 0.023

7.706 ± 0.038

4.962 ± 0.022

5.113 ± 0.027

8.043 ± 0.035

1.259 ± 0.015

1.998 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski