Pelolinea submarina

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; Pelolinea

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3066 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A347ZPX9|A0A347ZPX9_9CHLR PTS system IIB component (L-Asc family) OS=Pelolinea submarina OX=913107 GN=DFR64_2744 PE=4 SV=1
MM1 pKa = 7.56ISTRR5 pKa = 11.84SVRR8 pKa = 11.84LISVLFLATMLLTACGGSSTAAASDD33 pKa = 3.37WSNAASAEE41 pKa = 4.17AGGGMDD47 pKa = 4.55ALVAAAQAEE56 pKa = 4.63GEE58 pKa = 4.5LNVIALPEE66 pKa = 4.2DD67 pKa = 3.02WCNYY71 pKa = 9.42GGMIATFEE79 pKa = 4.28DD80 pKa = 4.11KK81 pKa = 11.3YY82 pKa = 10.84GIKK85 pKa = 10.4VNSITPEE92 pKa = 4.01AGSADD97 pKa = 3.94EE98 pKa = 4.2VQAIIDD104 pKa = 3.66NKK106 pKa = 9.46EE107 pKa = 3.97NKK109 pKa = 9.71GPQAPDD115 pKa = 3.49VIDD118 pKa = 3.4VGPAYY123 pKa = 10.28GPSSKK128 pKa = 10.92DD129 pKa = 3.18QDD131 pKa = 3.8LLAPYY136 pKa = 10.14KK137 pKa = 9.63VTTWDD142 pKa = 3.02SMTGVKK148 pKa = 10.37DD149 pKa = 3.26ADD151 pKa = 3.55GYY153 pKa = 11.78YY154 pKa = 8.04YY155 pKa = 10.56TDD157 pKa = 3.44YY158 pKa = 10.97NGVMVFEE165 pKa = 4.81VNTDD169 pKa = 3.06VVTDD173 pKa = 3.88VPQDD177 pKa = 3.7YY178 pKa = 11.51ADD180 pKa = 4.84LLDD183 pKa = 4.15PKK185 pKa = 11.33YY186 pKa = 10.64NGQVALAGDD195 pKa = 3.99PRR197 pKa = 11.84ASNQAAQTVYY207 pKa = 10.9AAALANGGSLDD218 pKa = 4.63DD219 pKa = 3.86IQPGLEE225 pKa = 3.97YY226 pKa = 10.64FKK228 pKa = 11.28ALNEE232 pKa = 4.26NGNLLPLIANTGSIAMGEE250 pKa = 4.29TPITFQWNYY259 pKa = 10.21LALANDD265 pKa = 4.29DD266 pKa = 4.55AFAGNPPLEE275 pKa = 4.12IVYY278 pKa = 7.33PTSVSWGGYY287 pKa = 6.89YY288 pKa = 10.33LQAISAYY295 pKa = 9.54APHH298 pKa = 7.24PAAARR303 pKa = 11.84LWQEE307 pKa = 3.52FLYY310 pKa = 10.77SDD312 pKa = 4.16EE313 pKa = 4.76GQTIWMTGYY322 pKa = 10.04CAPARR327 pKa = 11.84LADD330 pKa = 3.55MLEE333 pKa = 4.31RR334 pKa = 11.84GVVSADD340 pKa = 3.61LQTKK344 pKa = 10.36LPDD347 pKa = 3.65PSIIANAVVPDD358 pKa = 4.17GDD360 pKa = 3.74QLSAARR366 pKa = 11.84DD367 pKa = 3.84LIKK370 pKa = 10.51EE371 pKa = 3.93QWDD374 pKa = 3.98SVVGLDD380 pKa = 4.59IKK382 pKa = 10.73EE383 pKa = 3.96

Molecular weight: 40.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A347ZNM1|A0A347ZNM1_9CHLR Exodeoxyribonuclease 7 small subunit OS=Pelolinea submarina OX=913107 GN=xseB PE=3 SV=1
MM1 pKa = 7.57KK2 pKa = 10.49NLARR6 pKa = 11.84PILICTVILLIAVLLGIGVGSISISPQEE34 pKa = 4.07SLSILWQALRR44 pKa = 11.84GLPLDD49 pKa = 4.69GEE51 pKa = 4.76GQSIPSVILLTLRR64 pKa = 11.84LPRR67 pKa = 11.84TLLMLLAGAALSGSGCAYY85 pKa = 10.13QGLFRR90 pKa = 11.84NPLADD95 pKa = 4.3PYY97 pKa = 11.14LIGAASGAGVGAVAAMSLNWPSTTLGYY124 pKa = 10.44LAVPLASFAGSLLAVFLVFRR144 pKa = 11.84LARR147 pKa = 11.84VGRR150 pKa = 11.84TLPVTNLILAGVAVSSFATALSSFMMVNAGGEE182 pKa = 4.02LRR184 pKa = 11.84RR185 pKa = 11.84AFVWMLGGSTMSGWKK200 pKa = 9.19PVLGMLPYY208 pKa = 10.18TLVGLGALLALSYY221 pKa = 10.91KK222 pKa = 10.83LNILQLGDD230 pKa = 3.61EE231 pKa = 4.5QAQQLGIKK239 pKa = 9.39VSRR242 pKa = 11.84VRR244 pKa = 11.84TQIILAATLATAAAVAFSGIIGFVGLIIPHH274 pKa = 5.11ITRR277 pKa = 11.84RR278 pKa = 11.84LWGGDD283 pKa = 3.49MRR285 pKa = 11.84RR286 pKa = 11.84MLPLSMLCGGAFLVFADD303 pKa = 3.44VLARR307 pKa = 11.84VIMAPQEE314 pKa = 4.1LPVGIITALCGAPFFLFIMRR334 pKa = 11.84QSKK337 pKa = 9.71EE338 pKa = 4.14GSWW341 pKa = 3.34

Molecular weight: 35.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3066

0

3066

1049187

30

15355

342.2

37.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.721 ± 0.047

0.955 ± 0.021

5.624 ± 0.086

6.414 ± 0.058

4.207 ± 0.03

7.396 ± 0.101

1.816 ± 0.025

7.027 ± 0.039

4.778 ± 0.072

10.419 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.572 ± 0.037

4.112 ± 0.051

4.41 ± 0.056

3.897 ± 0.043

4.67 ± 0.073

6.183 ± 0.078

5.341 ± 0.087

6.86 ± 0.039

1.323 ± 0.022

3.276 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski