Loktanella phage pCB2051-A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4R174|M4R174_9CAUD Uncharacterized protein OS=Loktanella phage pCB2051-A OX=754044 GN=LOKG_00036 PE=4 SV=1
MM1 pKa = 7.2AQSPNNYY8 pKa = 8.79VLGRR12 pKa = 11.84GEE14 pKa = 4.06VWFDD18 pKa = 2.96QYY20 pKa = 12.15APGTLNTTGEE30 pKa = 4.54LYY32 pKa = 10.84LGNTPEE38 pKa = 4.25WNVSAEE44 pKa = 3.95ADD46 pKa = 3.68MLDD49 pKa = 3.89HH50 pKa = 6.63YY51 pKa = 11.49GSDD54 pKa = 2.95RR55 pKa = 11.84GIRR58 pKa = 11.84EE59 pKa = 3.75KK60 pKa = 10.92DD61 pKa = 3.11ASEE64 pKa = 3.88TLQVNRR70 pKa = 11.84TGSVITDD77 pKa = 3.82NISAEE82 pKa = 3.84NVAYY86 pKa = 10.06FFFGTTDD93 pKa = 3.37PFTVAGGAIAAEE105 pKa = 4.52SYY107 pKa = 11.39GPVTLGRR114 pKa = 11.84SYY116 pKa = 11.29QLGVTPTNPVGVQQVSLVSVTDD138 pKa = 3.39VGGTTTYY145 pKa = 11.07VEE147 pKa = 4.3GTDD150 pKa = 3.74YY151 pKa = 11.24EE152 pKa = 4.34VDD154 pKa = 3.68LDD156 pKa = 4.05LGRR159 pKa = 11.84IQILVGGDD167 pKa = 3.1IDD169 pKa = 4.06DD170 pKa = 4.0TDD172 pKa = 3.93MLEE175 pKa = 3.63ISYY178 pKa = 8.67TVTGYY183 pKa = 9.44TIDD186 pKa = 4.33RR187 pKa = 11.84IISGGVPIEE196 pKa = 4.01GQLRR200 pKa = 11.84YY201 pKa = 9.57IEE203 pKa = 4.42YY204 pKa = 10.59NPAGKK209 pKa = 10.06SRR211 pKa = 11.84IWVMPRR217 pKa = 11.84VKK219 pKa = 10.56LSPNGDD225 pKa = 3.71YY226 pKa = 10.83NLKK229 pKa = 10.57GDD231 pKa = 4.35DD232 pKa = 3.56WQQIPLNVEE241 pKa = 3.97VLKK244 pKa = 11.0KK245 pKa = 10.76GSLEE249 pKa = 4.26AIYY252 pKa = 10.24IDD254 pKa = 3.82GKK256 pKa = 11.02AVV258 pKa = 2.91

Molecular weight: 28.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4QPB8|M4QPB8_9CAUD Uncharacterized protein OS=Loktanella phage pCB2051-A OX=754044 GN=LOKG_00044 PE=4 SV=1
MM1 pKa = 7.27VNLGASKK8 pKa = 11.02VFFLSHH14 pKa = 6.53KK15 pKa = 9.91MLIINFNRR23 pKa = 11.84LSLQAARR30 pKa = 11.84SSAPMTGWGMNWNNSWGGATVLPSKK55 pKa = 10.86

Molecular weight: 6.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

17825

50

1611

234.5

26.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.229 ± 0.69

0.92 ± 0.127

7.153 ± 0.306

6.867 ± 0.25

3.837 ± 0.148

8.0 ± 0.204

1.835 ± 0.18

5.526 ± 0.187

5.683 ± 0.24

7.276 ± 0.248

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.647 ± 0.225

3.832 ± 0.141

4.533 ± 0.32

3.17 ± 0.152

5.997 ± 0.296

5.576 ± 0.202

6.182 ± 0.275

6.244 ± 0.201

1.56 ± 0.157

2.934 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski