Calothrix sp. PCC 7507

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Calotrichaceae; Calothrix; unclassified Calothrix

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5924 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9PC12|K9PC12_9CYAN DevC protein OS=Calothrix sp. PCC 7507 OX=99598 GN=Cal7507_0420 PE=4 SV=1
MM1 pKa = 7.66LLTATDD7 pKa = 3.59AGQIALEE14 pKa = 3.92FLMVDD19 pKa = 3.68WNISEE24 pKa = 4.45YY25 pKa = 10.69DD26 pKa = 3.85RR27 pKa = 11.84EE28 pKa = 4.2WFTIFNSRR36 pKa = 11.84LIGEE40 pKa = 4.48CWYY43 pKa = 10.14IVEE46 pKa = 4.75LGVAGFPDD54 pKa = 2.95RR55 pKa = 11.84WFIQVYY61 pKa = 8.38DD62 pKa = 4.33TGACDD67 pKa = 3.56PDD69 pKa = 3.65YY70 pKa = 11.31TFISPIRR77 pKa = 11.84GSDD80 pKa = 3.72GYY82 pKa = 11.67VDD84 pKa = 6.4LIDD87 pKa = 5.07LPQLVAEE94 pKa = 4.41VLVSEE99 pKa = 4.56RR100 pKa = 11.84NSRR103 pKa = 3.42

Molecular weight: 11.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9PMN2|K9PMN2_9CYAN Uncharacterized protein OS=Calothrix sp. PCC 7507 OX=99598 GN=Cal7507_3657 PE=4 SV=1
MM1 pKa = 7.08QRR3 pKa = 11.84TLGGTNRR10 pKa = 11.84KK11 pKa = 9.1RR12 pKa = 11.84KK13 pKa = 7.65RR14 pKa = 11.84TSGFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TPDD26 pKa = 3.21GRR28 pKa = 11.84NVISARR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.79KK37 pKa = 10.1GRR39 pKa = 11.84HH40 pKa = 5.0RR41 pKa = 11.84LSVV44 pKa = 3.12

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5924

0

5924

1845511

29

2879

311.5

34.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.272 ± 0.029

0.94 ± 0.011

4.81 ± 0.024

6.087 ± 0.036

3.936 ± 0.022

6.562 ± 0.036

1.849 ± 0.017

6.87 ± 0.026

4.865 ± 0.03

11.055 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.754 ± 0.013

4.379 ± 0.028

4.715 ± 0.021

5.569 ± 0.031

5.019 ± 0.023

6.435 ± 0.028

5.728 ± 0.028

6.65 ± 0.023

1.439 ± 0.014

3.066 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski