Silicimonas algicola

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Silicimonas

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4315 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A316G8Z5|A0A316G8Z5_9RHOB Glutamate 5-kinase OS=Silicimonas algicola OX=1826607 GN=proB PE=3 SV=1
MM1 pKa = 6.96TCKK4 pKa = 10.58ALIPGLLALATLSGCSGGGGGAVYY28 pKa = 10.94ADD30 pKa = 4.14GPSPASIDD38 pKa = 4.03EE39 pKa = 4.23IVGLQFPLRR48 pKa = 11.84SARR51 pKa = 11.84LTSDD55 pKa = 4.85DD56 pKa = 4.52GFTTANAGLSTYY68 pKa = 9.87TIEE71 pKa = 4.34FTSGTTANLTTPAGTVEE88 pKa = 4.12LTDD91 pKa = 3.65GGGMNFTGSLNGSDD105 pKa = 4.24YY106 pKa = 11.15FLTSLGWGHH115 pKa = 7.39DD116 pKa = 3.64FLEE119 pKa = 4.69GLGLEE124 pKa = 4.63VTDD127 pKa = 3.93GMGNVLVATGVFGLEE142 pKa = 4.01TRR144 pKa = 11.84PEE146 pKa = 4.4DD147 pKa = 3.62VPEE150 pKa = 4.22AAVVANFLGGSEE162 pKa = 4.08LHH164 pKa = 6.05GTVNDD169 pKa = 3.5VPFFDD174 pKa = 3.86TGAANLTADD183 pKa = 5.04FGAQTVGGNVFVGGGTTLAFDD204 pKa = 4.24GPVPIVGNTFSGDD217 pKa = 3.59LEE219 pKa = 4.45IFSATATLNEE229 pKa = 4.1GTATGRR235 pKa = 11.84FYY237 pKa = 11.68GDD239 pKa = 3.12AAQVVAGTYY248 pKa = 10.08SGSATEE254 pKa = 4.82AGSDD258 pKa = 3.27VDD260 pKa = 4.04FAGVFHH266 pKa = 7.6ADD268 pKa = 2.94

Molecular weight:
26.73 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A316GD04|A0A316GD04_9RHOB Uncharacterized protein DUF1446 OS=Silicimonas algicola OX=1826607 GN=C8D95_101634 PE=4 SV=1
MM1 pKa = 7.26LHH3 pKa = 5.01HH4 pKa = 6.98HH5 pKa = 6.47FRR7 pKa = 11.84RR8 pKa = 11.84FLDD11 pKa = 4.25ALDD14 pKa = 5.01DD15 pKa = 4.48LNPAQIEE22 pKa = 4.09DD23 pKa = 4.06AQTKK27 pKa = 9.83IRR29 pKa = 11.84DD30 pKa = 3.36IRR32 pKa = 11.84RR33 pKa = 11.84KK34 pKa = 8.1TEE36 pKa = 4.47AISEE40 pKa = 4.06IEE42 pKa = 3.7ARR44 pKa = 11.84TNRR47 pKa = 11.84EE48 pKa = 4.06HH49 pKa = 7.25KK50 pKa = 10.76CPFCGDD56 pKa = 3.07EE57 pKa = 5.39RR58 pKa = 11.84RR59 pKa = 11.84QKK61 pKa = 9.63WGRR64 pKa = 11.84TRR66 pKa = 11.84TKK68 pKa = 8.8IQRR71 pKa = 11.84YY72 pKa = 8.2RR73 pKa = 11.84CSGCRR78 pKa = 11.84KK79 pKa = 7.68TYY81 pKa = 10.22SGRR84 pKa = 11.84TGSAIGRR91 pKa = 11.84IHH93 pKa = 7.36RR94 pKa = 11.84PDD96 pKa = 3.69LFLVALRR103 pKa = 11.84DD104 pKa = 3.82MLDD107 pKa = 3.03ASAPQSVRR115 pKa = 11.84KK116 pKa = 9.05LARR119 pKa = 11.84QLDD122 pKa = 4.16LNKK125 pKa = 9.1YY126 pKa = 6.29TVWRR130 pKa = 11.84WRR132 pKa = 11.84MIVFSIIGSRR142 pKa = 11.84SVATSFSGIIEE153 pKa = 4.19ADD155 pKa = 3.05EE156 pKa = 4.5TYY158 pKa = 10.6QRR160 pKa = 11.84EE161 pKa = 4.19SRR163 pKa = 11.84KK164 pKa = 10.06GSRR167 pKa = 11.84EE168 pKa = 3.88WVHH171 pKa = 6.21HH172 pKa = 5.8SRR174 pKa = 11.84SAEE177 pKa = 4.25CYY179 pKa = 9.1SAAAPAVGRR188 pKa = 11.84LHH190 pKa = 7.69DD191 pKa = 4.36ARR193 pKa = 11.84AEE195 pKa = 3.74NDD197 pKa = 3.04ARR199 pKa = 11.84SFEE202 pKa = 4.38VATPILTVADD212 pKa = 4.07RR213 pKa = 11.84SGARR217 pKa = 11.84LFQRR221 pKa = 11.84LPNRR225 pKa = 11.84KK226 pKa = 8.75RR227 pKa = 11.84GTVEE231 pKa = 4.2RR232 pKa = 11.84AMQPLVPGDD241 pKa = 4.1AVLCSDD247 pKa = 4.83GGNGYY252 pKa = 10.43KK253 pKa = 10.19SLAAARR259 pKa = 11.84GLVHH263 pKa = 6.49FVVGSRR269 pKa = 11.84PGTRR273 pKa = 11.84VAAGCYY279 pKa = 9.58HH280 pKa = 6.13IQNVNSLHH288 pKa = 5.65ARR290 pKa = 11.84YY291 pKa = 9.74GKK293 pKa = 10.22FIRR296 pKa = 11.84PFCGPATKK304 pKa = 10.48NLNGYY309 pKa = 8.78IRR311 pKa = 11.84WLEE314 pKa = 3.69VRR316 pKa = 11.84LAGVRR321 pKa = 11.84PAEE324 pKa = 4.29VVRR327 pKa = 11.84ASS329 pKa = 3.1

Molecular weight:
37.17 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4315

0

4315

1295017

25

2773

300.1

32.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.386 ± 0.054

0.856 ± 0.012

6.189 ± 0.031

6.047 ± 0.033

3.725 ± 0.025

8.829 ± 0.033

2.008 ± 0.019

4.898 ± 0.027

2.712 ± 0.027

10.145 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.621 ± 0.018

2.277 ± 0.019

5.154 ± 0.026

2.694 ± 0.023

7.366 ± 0.035

5.256 ± 0.026

5.563 ± 0.025

7.699 ± 0.027

1.466 ± 0.015

2.109 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski