Bowmanella sp. JS7-9

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Bowmanella; unclassified Bowmanella

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2974 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q9XH50|A0A4Q9XH50_9ALTE Metal ABC transporter permease OS=Bowmanella sp. JS7-9 OX=1605368 GN=TK45_15905 PE=4 SV=1
MM1 pKa = 6.68NHH3 pKa = 6.79KK4 pKa = 10.46LITVSLIAAGVLAGCGGSNDD24 pKa = 4.51KK25 pKa = 10.58PLEE28 pKa = 4.12QPNPTFALAISDD40 pKa = 4.38APVDD44 pKa = 3.9DD45 pKa = 4.32LSEE48 pKa = 4.17VVVCFNAVEE57 pKa = 4.33LKK59 pKa = 10.67HH60 pKa = 6.91ADD62 pKa = 3.29GSASDD67 pKa = 3.5STFTVGTDD75 pKa = 3.51PQMLTANDD83 pKa = 3.32VCKK86 pKa = 10.79NDD88 pKa = 3.65AGEE91 pKa = 4.39VIPATVGINLLDD103 pKa = 3.75YY104 pKa = 10.44MGMASEE110 pKa = 4.55SLLSEE115 pKa = 4.17VSIAPGTYY123 pKa = 8.49NQLRR127 pKa = 11.84LVMADD132 pKa = 3.25GSYY135 pKa = 9.82GTVKK139 pKa = 9.6EE140 pKa = 4.35TGEE143 pKa = 4.52KK144 pKa = 9.35IDD146 pKa = 3.46VSVPSNEE153 pKa = 4.01LKK155 pKa = 10.88LDD157 pKa = 3.74GFTAALGNTLSFTLEE172 pKa = 3.42FDD174 pKa = 3.67LRR176 pKa = 11.84KK177 pKa = 10.55AMTNPVGQSGYY188 pKa = 8.38FLKK191 pKa = 10.7PRR193 pKa = 11.84GVRR196 pKa = 11.84LVDD199 pKa = 3.39NNQIGHH205 pKa = 4.92VQGTVDD211 pKa = 3.61EE212 pKa = 5.08TILVNNSCTVAPSDD226 pKa = 2.93ITTPVATVYY235 pKa = 10.54LYY237 pKa = 10.67QGVSLDD243 pKa = 4.07SNTLADD249 pKa = 3.5NGGAEE254 pKa = 4.2GVEE257 pKa = 4.4PFASTTVTFDD267 pKa = 2.97GAATYY272 pKa = 9.89SYY274 pKa = 10.72EE275 pKa = 3.88IGYY278 pKa = 9.4VPAGDD283 pKa = 3.77YY284 pKa = 9.26TLSLSCDD291 pKa = 3.4TVDD294 pKa = 5.85DD295 pKa = 4.85PEE297 pKa = 6.71ADD299 pKa = 4.0DD300 pKa = 7.01DD301 pKa = 4.17ITQLQQAEE309 pKa = 4.43VSVSANSTPVAVNFTAGG326 pKa = 3.08

Molecular weight: 33.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q9XT28|A0A4Q9XT28_9ALTE Uncharacterized protein OS=Bowmanella sp. JS7-9 OX=1605368 GN=TK45_01415 PE=4 SV=1
MM1 pKa = 7.0MWWLMICIVFAFGVEE16 pKa = 3.75AMTGFGSIVLALSLGALVYY35 pKa = 10.08SIPEE39 pKa = 4.25LVTLLVPLNLVMTLPLCIRR58 pKa = 11.84LRR60 pKa = 11.84QHH62 pKa = 5.25IQWRR66 pKa = 11.84LLLTRR71 pKa = 11.84LLPLMAVGTLLGQALLPWVNPDD93 pKa = 2.71WLKK96 pKa = 11.13LVFAMLITWFALRR109 pKa = 11.84ALLKK113 pKa = 10.36SQALALPTGIANGLITLAGVTHH135 pKa = 6.9GLFASGGPLLVYY147 pKa = 10.73ALARR151 pKa = 11.84SQLPKK156 pKa = 10.24AQFRR160 pKa = 11.84ATLLFVWLCLNSVLSLWFLFSGRR183 pKa = 11.84FEE185 pKa = 4.5GQWPALLQLVPAVLLGMWLGNRR207 pKa = 11.84LHH209 pKa = 6.83HH210 pKa = 7.04KK211 pKa = 10.02IDD213 pKa = 3.35AQRR216 pKa = 11.84FLPSVFAILLLVSVILLVTSLQQVAGG242 pKa = 3.85

Molecular weight: 26.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2974

0

2974

973382

38

4501

327.3

36.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.718 ± 0.047

1.0 ± 0.017

5.815 ± 0.037

5.652 ± 0.04

4.027 ± 0.025

7.01 ± 0.041

2.346 ± 0.025

5.751 ± 0.033

4.372 ± 0.042

10.711 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.654 ± 0.023

3.956 ± 0.03

4.097 ± 0.024

5.354 ± 0.048

5.126 ± 0.035

6.098 ± 0.036

5.048 ± 0.038

6.956 ± 0.04

1.325 ± 0.018

2.984 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski