Mycobacterium phage Thibault

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Omegavirus; Mycobacterium virus Thibault

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 216 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1FGQ2|G1FGQ2_9CAUD Uncharacterized protein OS=Mycobacterium phage Thibault OX=1052673 GN=189 PE=4 SV=1
MM1 pKa = 7.45SATADD6 pKa = 3.69TLVSAFADD14 pKa = 3.47LGEE17 pKa = 4.64PLDD20 pKa = 5.63DD21 pKa = 4.35EE22 pKa = 5.07ANCYY26 pKa = 10.75LEE28 pKa = 4.47VDD30 pKa = 3.71TEE32 pKa = 4.05EE33 pKa = 4.1RR34 pKa = 11.84QAIIVWVEE42 pKa = 3.24PLAFHH47 pKa = 7.63AEE49 pKa = 4.41FNGPRR54 pKa = 11.84QYY56 pKa = 11.42VATSSYY62 pKa = 10.41FVDD65 pKa = 4.18RR66 pKa = 11.84AMPDD70 pKa = 2.63HH71 pKa = 7.1WSTHH75 pKa = 4.29VDD77 pKa = 2.85ADD79 pKa = 3.39GWLTRR84 pKa = 11.84AVADD88 pKa = 4.69AIVARR93 pKa = 11.84LQGCIGARR101 pKa = 11.84GEE103 pKa = 4.29VYY105 pKa = 10.02EE106 pKa = 4.57LEE108 pKa = 4.42YY109 pKa = 11.32GGDD112 pKa = 3.55EE113 pKa = 4.04PNITFEE119 pKa = 4.3IVTSYY124 pKa = 11.56EE125 pKa = 3.87DD126 pKa = 4.98GEE128 pKa = 4.77TFDD131 pKa = 3.78HH132 pKa = 6.5WFDD135 pKa = 3.49RR136 pKa = 11.84VGWPIVATLRR146 pKa = 11.84NVTDD150 pKa = 4.35PGTFMSPYY158 pKa = 8.77LFAMASLEE166 pKa = 4.2VEE168 pKa = 4.43SS169 pKa = 4.53

Molecular weight: 18.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G1FGG1|G1FGG1_9CAUD Uncharacterized protein OS=Mycobacterium phage Thibault OX=1052673 GN=96 PE=4 SV=1
MM1 pKa = 7.55LAEE4 pKa = 4.49CWMPIPGLPGYY15 pKa = 7.93EE16 pKa = 3.76ASSLGRR22 pKa = 11.84VRR24 pKa = 11.84SLPRR28 pKa = 11.84RR29 pKa = 11.84ITQVRR34 pKa = 11.84YY35 pKa = 9.71GKK37 pKa = 9.73SHH39 pKa = 6.88VITAKK44 pKa = 10.55GRR46 pKa = 11.84VLKK49 pKa = 10.45PRR51 pKa = 11.84MINGYY56 pKa = 9.64LVVQPRR62 pKa = 11.84RR63 pKa = 11.84PDD65 pKa = 3.02HH66 pKa = 5.7STWSVGVHH74 pKa = 5.83RR75 pKa = 11.84LVLMAFSGVHH85 pKa = 6.31HH86 pKa = 6.87PTLDD90 pKa = 3.63CRR92 pKa = 11.84HH93 pKa = 6.28LNGDD97 pKa = 3.48RR98 pKa = 11.84MDD100 pKa = 4.02NRR102 pKa = 11.84PEE104 pKa = 3.7NLEE107 pKa = 3.56WGTRR111 pKa = 11.84LDD113 pKa = 3.6NLEE116 pKa = 4.0DD117 pKa = 3.6QKK119 pKa = 11.91LHH121 pKa = 5.68GTHH124 pKa = 7.04PSQRR128 pKa = 11.84LVTHH132 pKa = 6.7CPAGHH137 pKa = 7.02EE138 pKa = 4.06YY139 pKa = 10.27TPEE142 pKa = 3.43NTARR146 pKa = 11.84RR147 pKa = 11.84KK148 pKa = 9.47SDD150 pKa = 2.66GSRR153 pKa = 11.84RR154 pKa = 11.84CRR156 pKa = 11.84QCDD159 pKa = 2.95RR160 pKa = 11.84EE161 pKa = 4.15YY162 pKa = 11.05ARR164 pKa = 11.84EE165 pKa = 3.74YY166 pKa = 10.03QRR168 pKa = 11.84RR169 pKa = 11.84KK170 pKa = 9.31RR171 pKa = 11.84RR172 pKa = 11.84TRR174 pKa = 11.84GGLRR178 pKa = 11.84QEE180 pKa = 4.18

Molecular weight: 20.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

216

0

216

32860

18

1615

152.1

16.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.726 ± 0.269

1.522 ± 0.141

6.598 ± 0.156

7.021 ± 0.24

3.223 ± 0.095

8.509 ± 0.347

2.31 ± 0.133

4.848 ± 0.148

4.087 ± 0.18

8.138 ± 0.159

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.383 ± 0.083

3.281 ± 0.124

5.301 ± 0.165

3.086 ± 0.154

6.884 ± 0.239

5.53 ± 0.146

5.52 ± 0.239

6.838 ± 0.217

2.276 ± 0.119

2.921 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski