Virgibacillus necropolis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Virgibacillus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3964 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A221MD05|A0A221MD05_9BACI Glutamate dehydrogenase OS=Virgibacillus necropolis OX=163877 GN=CFK40_10955 PE=3 SV=1
MM1 pKa = 7.64AKK3 pKa = 8.85YY4 pKa = 9.04TIVDD8 pKa = 3.65KK9 pKa = 8.27EE10 pKa = 4.35TCIACGACGAAAPDD24 pKa = 3.33IYY26 pKa = 11.08DD27 pKa = 3.73YY28 pKa = 11.62DD29 pKa = 4.58DD30 pKa = 4.06EE31 pKa = 5.57GLAYY35 pKa = 10.36VVIDD39 pKa = 4.79DD40 pKa = 3.88NTGAAEE46 pKa = 3.82IPEE49 pKa = 4.28VFEE52 pKa = 4.42EE53 pKa = 4.32DD54 pKa = 3.18MVDD57 pKa = 3.62AFEE60 pKa = 4.84GCPTDD65 pKa = 4.53SIKK68 pKa = 10.72ISDD71 pKa = 3.9KK72 pKa = 10.97KK73 pKa = 11.26FNGDD77 pKa = 3.43PLRR80 pKa = 11.84FEE82 pKa = 5.68DD83 pKa = 4.37EE84 pKa = 4.09

Molecular weight: 9.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A221M834|A0A221M834_9BACI tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG OS=Virgibacillus necropolis OX=163877 GN=mnmG PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.21QPNNRR10 pKa = 11.84KK11 pKa = 8.85HH12 pKa = 6.37KK13 pKa = 9.52KK14 pKa = 7.68VHH16 pKa = 5.38GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.44VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.43GRR39 pKa = 11.84KK40 pKa = 8.78VLSAA44 pKa = 4.05

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3964

0

3964

1189348

27

1545

300.0

33.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.814 ± 0.039

0.687 ± 0.01

5.346 ± 0.029

7.228 ± 0.045

4.542 ± 0.03

6.952 ± 0.039

2.075 ± 0.021

8.19 ± 0.044

6.938 ± 0.04

9.515 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.777 ± 0.02

4.714 ± 0.027

3.592 ± 0.021

3.552 ± 0.028

3.72 ± 0.026

6.116 ± 0.028

5.614 ± 0.028

7.093 ± 0.024

1.026 ± 0.016

3.511 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski