Gulosibacter sp. YIM 102482-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Gulosibacter

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2531 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P3VXF6|A0A3P3VXF6_9MICO Elongation factor Tu OS=Gulosibacter sp. YIM 102482-1 OX=2488791 GN=tuf PE=3 SV=1
MM1 pKa = 7.47KK2 pKa = 10.54AHH4 pKa = 5.99GTALEE9 pKa = 4.34VTATGVRR16 pKa = 11.84ITVVGTKK23 pKa = 10.07QGDD26 pKa = 3.55PSGEE30 pKa = 3.8FSFDD34 pKa = 3.19VTGAGNDD41 pKa = 3.57LVLTPTGGAANDD53 pKa = 3.85GGEE56 pKa = 4.51LNTGGLGDD64 pKa = 3.49IHH66 pKa = 8.62LGMTRR71 pKa = 11.84ADD73 pKa = 3.38AVSGGWYY80 pKa = 9.87VDD82 pKa = 4.38DD83 pKa = 6.6GGFCPQPSDD92 pKa = 3.2AAVAMGATYY101 pKa = 10.25LYY103 pKa = 10.64FFGPGDD109 pKa = 3.53TLSIVGTTDD118 pKa = 2.71ASVRR122 pKa = 11.84TPSGAHH128 pKa = 5.17VGMTLSEE135 pKa = 4.09LQATYY140 pKa = 10.75GSQLVALDD148 pKa = 3.88TNVMTGAPGWGVAADD163 pKa = 4.44DD164 pKa = 4.5GNSIVFQMDD173 pKa = 2.96AGGSAVEE180 pKa = 3.99VMYY183 pKa = 10.72AQSGPPALNPGVGNCC198 pKa = 4.51

Molecular weight: 19.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P3VX52|A0A3P3VX52_9MICO MBL fold metallo-hydrolase OS=Gulosibacter sp. YIM 102482-1 OX=2488791 GN=EG850_09825 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.79HH17 pKa = 5.67RR18 pKa = 11.84KK19 pKa = 8.31LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2531

0

2531

839896

32

2286

331.8

35.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.815 ± 0.074

0.541 ± 0.012

6.159 ± 0.045

6.444 ± 0.047

3.249 ± 0.03

8.532 ± 0.044

2.041 ± 0.023

4.91 ± 0.035

2.292 ± 0.032

9.904 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.949 ± 0.019

2.405 ± 0.03

5.258 ± 0.037

3.135 ± 0.035

7.158 ± 0.055

5.41 ± 0.032

5.965 ± 0.041

8.353 ± 0.043

1.468 ± 0.02

2.013 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski