Bacteroides sp. CAG:530

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; environmental samples

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2669 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6DC72|R6DC72_9BACE Thioredoxin OS=Bacteroides sp. CAG:530 OX=1262741 GN=BN697_02022 PE=3 SV=1
MM1 pKa = 7.59KK2 pKa = 10.21KK3 pKa = 10.41YY4 pKa = 10.38ICTVCDD10 pKa = 3.09WVYY13 pKa = 11.11DD14 pKa = 4.08PEE16 pKa = 5.57VGDD19 pKa = 4.3PDD21 pKa = 4.84AGIAPGTAFEE31 pKa = 5.37DD32 pKa = 4.31LPEE35 pKa = 4.78DD36 pKa = 3.72WVCPVCGVGKK46 pKa = 10.48EE47 pKa = 3.98DD48 pKa = 4.07FKK50 pKa = 11.28PVEE53 pKa = 4.15EE54 pKa = 4.3

Molecular weight: 5.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6CZ27|R6CZ27_9BACE Two-component system sensor histidine kinase/response regulator hybrid (One-component system) OS=Bacteroides sp. CAG:530 OX=1262741 GN=BN697_00375 PE=4 SV=1
MM1 pKa = 7.18NWIEE5 pKa = 4.35HH6 pKa = 4.53VTIIDD11 pKa = 3.89LLIKK15 pKa = 10.19GFVVGVVVSAPLGPVGVLCIQRR37 pKa = 11.84TLNKK41 pKa = 9.74GRR43 pKa = 11.84WFGFVTGLGAALSDD57 pKa = 3.03ICYY60 pKa = 10.84ALITGYY66 pKa = 11.18GMSFMDD72 pKa = 4.36EE73 pKa = 5.15LILKK77 pKa = 9.19HH78 pKa = 6.72QMFLQIVGSIMLFCFGIYY96 pKa = 7.6TFRR99 pKa = 11.84SNPVRR104 pKa = 11.84SLRR107 pKa = 11.84PTSTARR113 pKa = 11.84GTYY116 pKa = 9.47LHH118 pKa = 6.55NFVTAFFVTFSNPLIIFLFIGLFARR143 pKa = 11.84FSFVMPGSPLGFQLVGYY160 pKa = 7.88WAIVLGAVTWWFGITYY176 pKa = 8.7FVNKK180 pKa = 9.0VRR182 pKa = 11.84ARR184 pKa = 11.84FNVRR188 pKa = 11.84GIWMLNRR195 pKa = 11.84IIGIVVIIASVAGVILTFSGKK216 pKa = 9.95SLSS219 pKa = 3.58

Molecular weight: 24.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2669

0

2669

984630

29

1629

368.9

41.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.199 ± 0.041

1.313 ± 0.017

5.724 ± 0.034

6.38 ± 0.039

4.516 ± 0.027

6.798 ± 0.038

1.921 ± 0.02

6.716 ± 0.042

6.855 ± 0.039

8.835 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.869 ± 0.022

5.28 ± 0.04

3.656 ± 0.022

3.402 ± 0.025

4.428 ± 0.033

6.212 ± 0.045

5.569 ± 0.035

6.571 ± 0.036

1.272 ± 0.019

4.48 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski