Hubei sobemo-like virus 43

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KE23|A0A1L3KE23_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 43 OX=1923231 PE=4 SV=1
MM1 pKa = 7.48PRR3 pKa = 11.84GGAVDD8 pKa = 3.73EE9 pKa = 4.32LASLKK14 pKa = 10.67AHH16 pKa = 6.76AEE18 pKa = 3.97FFRR21 pKa = 11.84QSRR24 pKa = 11.84EE25 pKa = 3.71SGLEE29 pKa = 3.71VEE31 pKa = 4.43PLDD34 pKa = 4.97RR35 pKa = 11.84EE36 pKa = 4.51RR37 pKa = 11.84ILHH40 pKa = 5.65ACEE43 pKa = 3.37TAYY46 pKa = 10.62AAARR50 pKa = 11.84YY51 pKa = 8.55SLPSDD56 pKa = 3.18WLEE59 pKa = 3.67KK60 pKa = 7.99THH62 pKa = 7.15FYY64 pKa = 10.68RR65 pKa = 11.84VVYY68 pKa = 10.17DD69 pKa = 3.44LEE71 pKa = 4.62FRR73 pKa = 11.84SSPGYY78 pKa = 9.21PYY80 pKa = 10.83CLASSTIGDD89 pKa = 3.53WLGWNGVGCDD99 pKa = 3.78GEE101 pKa = 4.42KK102 pKa = 10.88LEE104 pKa = 4.7EE105 pKa = 3.93LWFDD109 pKa = 3.46VLAFLSGEE117 pKa = 3.67RR118 pKa = 11.84DD119 pKa = 2.9SFYY122 pKa = 11.06RR123 pKa = 11.84VFIKK127 pKa = 10.6SEE129 pKa = 3.68PHH131 pKa = 4.97TLAKK135 pKa = 9.96RR136 pKa = 11.84DD137 pKa = 3.45AGRR140 pKa = 11.84WRR142 pKa = 11.84LIICPPLYY150 pKa = 10.51EE151 pKa = 4.04QVAWTMVFGPGNDD164 pKa = 3.44RR165 pKa = 11.84EE166 pKa = 4.29IEE168 pKa = 4.48TVGRR172 pKa = 11.84TPSMQGMSLPGGLWKK187 pKa = 10.54DD188 pKa = 3.15WLAIFQQRR196 pKa = 11.84GLNVAMDD203 pKa = 3.96KK204 pKa = 11.11SAWDD208 pKa = 3.55WTAHH212 pKa = 4.9EE213 pKa = 4.63QLISMDD219 pKa = 3.28LEE221 pKa = 4.15LRR223 pKa = 11.84HH224 pKa = 6.55RR225 pKa = 11.84LLTGPNRR232 pKa = 11.84GRR234 pKa = 11.84WRR236 pKa = 11.84QLAEE240 pKa = 3.86RR241 pKa = 11.84LYY243 pKa = 11.11DD244 pKa = 3.44GAFNHH249 pKa = 6.46PRR251 pKa = 11.84LVLSSGAVYY260 pKa = 10.1QQTEE264 pKa = 4.22PGVMKK269 pKa = 10.25SGCVNTISSNSHH281 pKa = 4.15MQVFVHH287 pKa = 6.68CLACLRR293 pKa = 11.84YY294 pKa = 9.4GLPLTPLPVAVGDD307 pKa = 4.13DD308 pKa = 3.99TLSSLSNLVEE318 pKa = 3.72PSAYY322 pKa = 9.93RR323 pKa = 11.84FTGAVVKK330 pKa = 10.47EE331 pKa = 4.29VDD333 pKa = 3.62LDD335 pKa = 3.55MHH337 pKa = 6.34FVGHH341 pKa = 6.54RR342 pKa = 11.84WYY344 pKa = 10.33EE345 pKa = 4.07SGPVPTYY352 pKa = 9.63SAKK355 pKa = 10.36HH356 pKa = 4.56FVRR359 pKa = 11.84FAVTGADD366 pKa = 3.69YY367 pKa = 11.42VPDD370 pKa = 4.3FLDD373 pKa = 3.81SMVRR377 pKa = 11.84LYY379 pKa = 11.02AHH381 pKa = 7.05HH382 pKa = 6.99EE383 pKa = 4.26GFQKK387 pKa = 9.49VWRR390 pKa = 11.84WLAYY394 pKa = 9.91RR395 pKa = 11.84RR396 pKa = 11.84GIDD399 pKa = 3.53LPSEE403 pKa = 4.08AFVKK407 pKa = 10.43FWYY410 pKa = 9.64DD411 pKa = 3.35YY412 pKa = 10.47PDD414 pKa = 5.31DD415 pKa = 4.61VIQYY419 pKa = 9.62YY420 pKa = 10.36GG421 pKa = 3.21

Molecular weight: 48.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KE23|A0A1L3KE23_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 43 OX=1923231 PE=4 SV=1
MM1 pKa = 6.3TTASFMFALARR12 pKa = 11.84KK13 pKa = 8.29QLPTAPPNPGWGIGLARR30 pKa = 11.84PTLRR34 pKa = 11.84EE35 pKa = 3.76GLVLVGGVAATAASGYY51 pKa = 10.3AVYY54 pKa = 10.61SVGCWTMSACRR65 pKa = 11.84RR66 pKa = 11.84LRR68 pKa = 11.84SWWAKK73 pKa = 8.46PLKK76 pKa = 10.11LRR78 pKa = 11.84PAPSGPVVGEE88 pKa = 4.18CVPEE92 pKa = 4.13SAVPGSEE99 pKa = 4.38EE100 pKa = 5.18RR101 pKa = 11.84PMTPPKK107 pKa = 10.45GQALVGFSDD116 pKa = 4.53GSSFMVVGCAVRR128 pKa = 11.84MEE130 pKa = 4.81DD131 pKa = 3.31WLVMPDD137 pKa = 3.51HH138 pKa = 6.87VKK140 pKa = 10.6SALGDD145 pKa = 3.23RR146 pKa = 11.84RR147 pKa = 11.84LEE149 pKa = 3.98IRR151 pKa = 11.84SMDD154 pKa = 3.59HH155 pKa = 6.13KK156 pKa = 10.89RR157 pKa = 11.84VVTLHH162 pKa = 5.53NAEE165 pKa = 4.13VEE167 pKa = 4.04AMQLVDD173 pKa = 3.56TDD175 pKa = 4.34LLAVQLSPARR185 pKa = 11.84FSEE188 pKa = 3.84IGLAKK193 pKa = 9.64VTVGPNLAEE202 pKa = 4.01KK203 pKa = 10.35FGAFSAIVGAFGKK216 pKa = 9.0GTTGTVKK223 pKa = 10.27HH224 pKa = 5.98AQLFGKK230 pKa = 8.15ITYY233 pKa = 8.39TGSTFKK239 pKa = 10.63GYY241 pKa = 10.62SGAAYY246 pKa = 8.37MAGTQLVGIHH256 pKa = 5.68LHH258 pKa = 6.27GGTVNAGYY266 pKa = 9.72SASYY270 pKa = 9.02VLAMLKK276 pKa = 10.6HH277 pKa = 5.6MFRR280 pKa = 11.84IKK282 pKa = 10.91DD283 pKa = 3.46EE284 pKa = 5.24GSDD287 pKa = 3.23EE288 pKa = 4.03WLEE291 pKa = 3.9GMRR294 pKa = 11.84RR295 pKa = 11.84QGAEE299 pKa = 3.92VVVDD303 pKa = 3.59QDD305 pKa = 3.03WRR307 pKa = 11.84DD308 pKa = 3.24MDD310 pKa = 3.62EE311 pKa = 3.99CRR313 pKa = 11.84IRR315 pKa = 11.84VAGRR319 pKa = 11.84YY320 pKa = 9.18HH321 pKa = 7.23IIGVDD326 pKa = 3.3TMSRR330 pKa = 11.84VYY332 pKa = 10.58GADD335 pKa = 2.56WRR337 pKa = 11.84RR338 pKa = 11.84SGKK341 pKa = 10.32RR342 pKa = 11.84NLKK345 pKa = 8.31TDD347 pKa = 3.48RR348 pKa = 11.84FVDD351 pKa = 4.1LEE353 pKa = 4.46SCVLPGEE360 pKa = 4.34SSEE363 pKa = 4.29TLSGGSRR370 pKa = 11.84VSVPSNPSLAEE381 pKa = 3.81LANQLTLHH389 pKa = 5.99EE390 pKa = 4.42LEE392 pKa = 4.46KK393 pKa = 10.89LRR395 pKa = 11.84EE396 pKa = 3.9RR397 pKa = 11.84LALRR401 pKa = 11.84AKK403 pKa = 10.34DD404 pKa = 3.71LRR406 pKa = 11.84ASATGSRR413 pKa = 11.84AA414 pKa = 3.06

Molecular weight: 44.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

835

414

421

417.5

46.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.862 ± 1.127

1.557 ± 0.08

5.269 ± 0.681

5.868 ± 0.231

3.832 ± 0.69

9.102 ± 0.95

2.754 ± 0.429

2.515 ± 0.074

3.593 ± 0.558

9.82 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.994 ± 0.465

1.796 ± 0.078

5.15 ± 0.236

2.515 ± 0.252

7.545 ± 0.136

7.545 ± 0.315

4.671 ± 0.654

8.263 ± 0.498

2.754 ± 0.608

3.593 ± 1.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski