Xanthomonas phage Xoo-sp14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 251 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7H0XA33|A0A7H0XA33_9CAUD Uncharacterized protein OS=Xanthomonas phage Xoo-sp14 OX=2769337 GN=Xoosp14_21 PE=4 SV=1
MM1 pKa = 7.23FVGEE5 pKa = 4.84VIFQTFSEE13 pKa = 5.07GIIEE17 pKa = 4.44DD18 pKa = 4.56GYY20 pKa = 11.13FEE22 pKa = 4.33EE23 pKa = 4.98TVGEE27 pKa = 4.02AMYY30 pKa = 8.69EE31 pKa = 3.91TFYY34 pKa = 11.37EE35 pKa = 4.23NNFNDD40 pKa = 4.47VIQDD44 pKa = 3.65TQILEE49 pKa = 4.21WVRR52 pKa = 11.84NVYY55 pKa = 11.05YY56 pKa = 9.64MVAPATVRR64 pKa = 11.84VVSGNGFIISGFGFARR80 pKa = 11.84SFGDD84 pKa = 3.23DD85 pKa = 2.64AVYY88 pKa = 10.11FFEE91 pKa = 4.44EE92 pKa = 3.81TDD94 pKa = 3.36LRR96 pKa = 11.84HH97 pKa = 5.91

Molecular weight: 11.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7H0XAK1|A0A7H0XAK1_9CAUD Putative terminase large subunit OS=Xanthomonas phage Xoo-sp14 OX=2769337 GN=Xoosp14_189 PE=4 SV=1
MM1 pKa = 7.3FVINLFDD8 pKa = 3.4VLFAFIMSAPLVFWGWVMGRR28 pKa = 11.84TWSKK32 pKa = 10.82SKK34 pKa = 10.71ASRR37 pKa = 11.84SIDD40 pKa = 3.78TFWTKK45 pKa = 9.62ATLALQHH52 pKa = 6.14GDD54 pKa = 2.8KK55 pKa = 10.41RR56 pKa = 11.84YY57 pKa = 9.49RR58 pKa = 11.84AHH60 pKa = 6.58ALVWNGVFFGLIAWGILVPLLIAMFWSRR88 pKa = 11.84SWIVLIPSGISLYY101 pKa = 10.2PILSMSGYY109 pKa = 8.45LTWCSTRR116 pKa = 11.84VSTSAA121 pKa = 3.58

Molecular weight: 13.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

251

0

251

70486

37

2420

280.8

31.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.673 ± 0.221

0.749 ± 0.04

6.444 ± 0.11

6.559 ± 0.159

3.903 ± 0.078

6.817 ± 0.182

2.122 ± 0.082

5.444 ± 0.095

5.105 ± 0.132

8.844 ± 0.135

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.04 ± 0.076

4.581 ± 0.089

4.5 ± 0.099

3.459 ± 0.088

6.142 ± 0.133

6.022 ± 0.109

6.001 ± 0.111

7.084 ± 0.133

1.077 ± 0.047

3.435 ± 0.091
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski