Actinotignum schaalii FB123-CNA-2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinotignum; Actinotignum schaalii

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1726 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S2WF00|S2WF00_9ACTO Uncharacterized protein OS=Actinotignum schaalii FB123-CNA-2 OX=883067 GN=HMPREF9237_01112 PE=4 SV=1
MM1 pKa = 7.77AYY3 pKa = 10.34VITLPCVDD11 pKa = 3.81TKK13 pKa = 11.45DD14 pKa = 3.46RR15 pKa = 11.84ACVDD19 pKa = 3.91EE20 pKa = 5.5CPVDD24 pKa = 4.37CIYY27 pKa = 11.15EE28 pKa = 4.18GDD30 pKa = 3.56RR31 pKa = 11.84TLYY34 pKa = 9.81IHH36 pKa = 7.52PDD38 pKa = 3.07EE39 pKa = 5.5CVDD42 pKa = 4.9CGACEE47 pKa = 3.78PACPVEE53 pKa = 4.4AIYY56 pKa = 11.26YY57 pKa = 9.25EE58 pKa = 4.57DD59 pKa = 4.89DD60 pKa = 3.9VPEE63 pKa = 4.12EE64 pKa = 4.08WSVFTAVNAEE74 pKa = 4.07FFSEE78 pKa = 4.21LGSPGGAQSLGPTGTDD94 pKa = 2.96HH95 pKa = 7.48PYY97 pKa = 10.77VAGLPANVNDD107 pKa = 3.99EE108 pKa = 4.37SS109 pKa = 3.78

Molecular weight: 11.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S2W118|S2W118_9ACTO 50S ribosomal protein L16 OS=Actinotignum schaalii FB123-CNA-2 OX=883067 GN=rplP PE=3 SV=1
MM1 pKa = 7.82IDD3 pKa = 4.49FISPLEE9 pKa = 3.86LWGTYY14 pKa = 8.08IFRR17 pKa = 11.84TMLIGTTLIGVFSGVAGCLLYY38 pKa = 10.72LRR40 pKa = 11.84RR41 pKa = 11.84QSLVSDD47 pKa = 4.36VIGHH51 pKa = 5.43SSILGVVAAFTVATLLGADD70 pKa = 3.8GRR72 pKa = 11.84SMVILVLGASAAAMCAVVLTNIIVAHH98 pKa = 6.08SKK100 pKa = 10.12VKK102 pKa = 10.22PDD104 pKa = 3.13AAMAICLSLFYY115 pKa = 10.79GGGMVCLRR123 pKa = 11.84LLTHH127 pKa = 5.95SHH129 pKa = 6.38LPNRR133 pKa = 11.84GGIDD137 pKa = 2.84SYY139 pKa = 11.6MFGNAANVRR148 pKa = 11.84AGDD151 pKa = 3.86LVTIAVFGLLTVAVILAFFKK171 pKa = 10.45EE172 pKa = 4.17IKK174 pKa = 10.34LVTFDD179 pKa = 4.25PVTAQLQGFSPRR191 pKa = 11.84VLTPLMLVLVTIAIVIGIKK210 pKa = 10.52GVGLILMVAFAIMPAAAARR229 pKa = 11.84QWTHH233 pKa = 5.98RR234 pKa = 11.84MSSMMVLAGIFGGLAGAAGAYY255 pKa = 10.44LSVCLGKK262 pKa = 10.63VPTGPVVVIHH272 pKa = 6.3LFVIFALSLLAAPRR286 pKa = 11.84RR287 pKa = 11.84SIVVVALRR295 pKa = 11.84QRR297 pKa = 11.84RR298 pKa = 11.84LRR300 pKa = 11.84EE301 pKa = 3.95DD302 pKa = 3.2LVARR306 pKa = 11.84ARR308 pKa = 11.84AATAAPVTGTVTATAAVQKK327 pKa = 10.54SATTPAPAAPAGVAAPAGSEE347 pKa = 3.77RR348 pKa = 11.84SEE350 pKa = 3.97RR351 pKa = 3.72

Molecular weight: 36.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1726

0

1726

612490

23

3016

354.9

38.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.882 ± 0.075

0.745 ± 0.016

5.496 ± 0.051

6.205 ± 0.064

3.116 ± 0.037

8.687 ± 0.053

2.035 ± 0.028

4.885 ± 0.048

2.746 ± 0.057

9.526 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.029 ± 0.026

2.657 ± 0.038

5.407 ± 0.054

3.0 ± 0.031

6.928 ± 0.074

5.83 ± 0.042

6.226 ± 0.055

7.89 ± 0.057

1.362 ± 0.022

2.349 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski