Rousettus bat coronavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Nobecovirus; Rousettus bat coronavirus GCCDC1

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B3Q5X0|A0A1B3Q5X0_9BETC Nucleocapsid protein OS=Rousettus bat coronavirus OX=1892416 GN=N PE=4 SV=1
MM1 pKa = 7.25YY2 pKa = 9.96EE3 pKa = 4.2LVGTDD8 pKa = 3.36TSVLIANVLVLIVLCVCIVIVGCAVLLILQFIVSTCTCFFTSVCKK53 pKa = 8.51PTVYY57 pKa = 9.99IYY59 pKa = 11.41NKK61 pKa = 9.95FKK63 pKa = 10.99YY64 pKa = 9.92DD65 pKa = 3.44SLSNEE70 pKa = 3.97QEE72 pKa = 4.02EE73 pKa = 4.51LLLL76 pKa = 5.14

Molecular weight: 8.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B3IZG9|A0A1B3IZG9_9BETC NS7a protein OS=Rousettus bat coronavirus OX=1892416 GN=NS7a PE=4 SV=1
MM1 pKa = 7.7GDD3 pKa = 3.94CNGMWSIFGSTNCNSAKK20 pKa = 9.12NTAGGNLEE28 pKa = 4.09ATNVLITYY36 pKa = 8.92GPYY39 pKa = 9.89LAAIVGLLLLVCLFYY54 pKa = 11.06CCYY57 pKa = 10.73LKK59 pKa = 10.88FKK61 pKa = 10.82ASQAKK66 pKa = 7.87KK67 pKa = 8.32TYY69 pKa = 9.19RR70 pKa = 11.84KK71 pKa = 9.57EE72 pKa = 4.31LISLTTRR79 pKa = 11.84QIYY82 pKa = 10.13APPRR86 pKa = 11.84EE87 pKa = 4.14ISHH90 pKa = 5.44VV91 pKa = 3.48

Molecular weight: 10.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

9851

76

6973

985.1

109.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.654 ± 0.331

3.238 ± 0.298

5.045 ± 0.501

4.223 ± 0.291

4.446 ± 0.313

6.223 ± 0.276

1.979 ± 0.344

4.132 ± 0.541

4.883 ± 0.705

9.644 ± 0.524

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.223 ± 0.213

4.781 ± 0.679

4.599 ± 0.355

3.441 ± 0.315

4.101 ± 0.583

6.761 ± 0.351

6.74 ± 0.414

9.928 ± 0.635

1.33 ± 0.2

4.629 ± 0.553
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski