Bacillus freudenreichii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3998 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A448FMX8|A0A448FMX8_9BACI Uncharacterized conserved protein contains double-stranded beta-helix domain OS=Bacillus freudenreichii OX=1783501 GN=NCTC4823_03986 PE=4 SV=1
MM1 pKa = 7.66AKK3 pKa = 10.41YY4 pKa = 10.62SIVDD8 pKa = 3.24QDD10 pKa = 3.56TCIACGACSEE20 pKa = 4.26TAPDD24 pKa = 4.06IFDD27 pKa = 4.69HH28 pKa = 6.62NDD30 pKa = 2.92SGFSFVKK37 pKa = 10.67LDD39 pKa = 4.02DD40 pKa = 4.06NQGTAEE46 pKa = 4.28IPDD49 pKa = 3.99DD50 pKa = 4.53LEE52 pKa = 5.7YY53 pKa = 11.16DD54 pKa = 4.94LEE56 pKa = 4.63DD57 pKa = 4.31ACDD60 pKa = 3.85GCPTEE65 pKa = 4.62SIKK68 pKa = 11.16VADD71 pKa = 4.21TPFKK75 pKa = 10.47KK76 pKa = 10.67DD77 pKa = 3.26PVEE80 pKa = 3.86AA81 pKa = 4.79

Molecular weight: 8.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S4QD27|A0A3S4QD27_9BACI Membrane protein OS=Bacillus freudenreichii OX=1783501 GN=NCTC4823_04234 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.59RR3 pKa = 11.84TFQPNKK9 pKa = 8.24RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84SKK14 pKa = 9.58VHH16 pKa = 5.76GFRR19 pKa = 11.84TRR21 pKa = 11.84MSSKK25 pKa = 8.21TGRR28 pKa = 11.84RR29 pKa = 11.84ILAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.84GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3998

0

3998

1178504

29

3470

294.8

32.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.38 ± 0.039

0.716 ± 0.012

5.184 ± 0.027

7.592 ± 0.052

4.547 ± 0.034

7.218 ± 0.04

2.009 ± 0.019

7.809 ± 0.037

6.883 ± 0.035

9.652 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.902 ± 0.021

4.159 ± 0.027

3.797 ± 0.023

3.403 ± 0.028

4.224 ± 0.032

5.924 ± 0.026

5.223 ± 0.029

7.03 ± 0.031

1.005 ± 0.015

3.341 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski