Acidianus rod-shaped virus 3

Taxonomy: Viruses; Adnaviria; Zilligvirae; Taleaviricota; Tokiviricetes; Ligamenvirales; Rudiviridae; Hoswirudivirus; Hoswirudivirus ARV3

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 33 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3VYK6|A0A6M3VYK6_9VIRU Uncharacterized protein OS=Acidianus rod-shaped virus 3 OX=2730617 GN=ARV3_gp09 PE=4 SV=1
MM1 pKa = 7.45ATPLNHH7 pKa = 6.06VSSNNVISSSAWNQAISDD25 pKa = 3.99LEE27 pKa = 4.35YY28 pKa = 10.23IYY30 pKa = 10.94SEE32 pKa = 4.24YY33 pKa = 9.57QTILNTIQQLQNINSQITASNYY55 pKa = 5.96QTEE58 pKa = 4.38ATDD61 pKa = 3.24ACNAIQNLFNYY72 pKa = 8.25LQSPTLLSSFPDD84 pKa = 4.13YY85 pKa = 9.91ITNIPPAVTGGVLFASMMNSLTYY108 pKa = 10.45AVEE111 pKa = 3.88KK112 pKa = 10.15LYY114 pKa = 11.26NSIMPKK120 pKa = 9.67PAPQISTVAGGDD132 pKa = 3.15IVTARR137 pKa = 11.84HH138 pKa = 4.91WNKK141 pKa = 9.84IVDD144 pKa = 4.64TINTLLNLFSPTIFANRR161 pKa = 11.84YY162 pKa = 3.27TTINVNFPFIFLYY175 pKa = 10.88LNEE178 pKa = 4.85SGVSVNTDD186 pKa = 3.0GPINTMFITGVTQQTYY202 pKa = 10.51LEE204 pKa = 4.49YY205 pKa = 10.91YY206 pKa = 10.1FYY208 pKa = 11.25GPNPITNLFLCAEE221 pKa = 4.25TSEE224 pKa = 4.31GASYY228 pKa = 11.86NNMFIQNIYY237 pKa = 10.01IYY239 pKa = 9.52QQNTYY244 pKa = 10.86YY245 pKa = 10.99YY246 pKa = 9.91FLDD249 pKa = 3.56NSVTQNVYY257 pKa = 10.73AYY259 pKa = 10.64NEE261 pKa = 4.5DD262 pKa = 3.51IYY264 pKa = 11.5LVEE267 pKa = 4.32EE268 pKa = 4.01NNAYY272 pKa = 9.05VQNAYY277 pKa = 9.72IYY279 pKa = 9.84GAYY282 pKa = 10.3AEE284 pKa = 4.66IEE286 pKa = 4.41TYY288 pKa = 11.33DD289 pKa = 3.61NAIADD294 pKa = 3.94NIYY297 pKa = 9.52TYY299 pKa = 10.59GYY301 pKa = 9.29KK302 pKa = 10.09SAVYY306 pKa = 9.91VEE308 pKa = 4.48NSSTVNNIYY317 pKa = 10.52VYY319 pKa = 11.22ADD321 pKa = 3.09YY322 pKa = 10.67GYY324 pKa = 11.11LYY326 pKa = 10.24VYY328 pKa = 10.44DD329 pKa = 4.0YY330 pKa = 10.99ATVEE334 pKa = 4.09NIYY337 pKa = 10.79VFGNEE342 pKa = 4.48GIVEE346 pKa = 4.3ASSPAIVQNLYY357 pKa = 10.95LCGGDD362 pKa = 4.14NSIFPYY368 pKa = 10.67NVINLYY374 pKa = 10.45LCNSNNYY381 pKa = 9.36ISGGSPNIITDD392 pKa = 3.54SSVCNQICGSNN403 pKa = 3.11

Molecular weight: 45.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3VXJ7|A0A6M3VXJ7_9VIRU Uncharacterized protein OS=Acidianus rod-shaped virus 3 OX=2730617 GN=ARV3_gp24 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.04GHH5 pKa = 5.62TPRR8 pKa = 11.84SYY10 pKa = 9.8SQRR13 pKa = 11.84YY14 pKa = 8.31AKK16 pKa = 10.05WSGKK20 pKa = 7.77FTAFSNPTVASTILTNVDD38 pKa = 3.23EE39 pKa = 4.45VAKK42 pKa = 10.83EE43 pKa = 3.81NFQTNVPKK51 pKa = 10.31FASVNDD57 pKa = 3.59QVSAVLTQYY66 pKa = 11.31GVTGPSRR73 pKa = 11.84AIYY76 pKa = 9.99QGFGLKK82 pKa = 9.31IARR85 pKa = 11.84ALNRR89 pKa = 11.84IGSGPALTNMIAGLKK104 pKa = 9.76AYY106 pKa = 9.88YY107 pKa = 9.89ISAFNANPTILDD119 pKa = 3.44AVTNIITGSPNGYY132 pKa = 9.96VSS134 pKa = 3.24

Molecular weight: 14.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

33

0

33

6866

51

1115

208.1

23.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.913 ± 0.388

0.845 ± 0.225

5.229 ± 0.357

6.408 ± 0.545

5.316 ± 0.291

4.369 ± 0.307

1.398 ± 0.256

9.117 ± 0.419

7.574 ± 0.83

9.074 ± 0.616

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.908 ± 0.205

6.569 ± 0.674

3.758 ± 0.277

4.967 ± 0.39

2.811 ± 0.383

6.379 ± 0.554

5.243 ± 0.464

6.19 ± 0.322

0.757 ± 0.115

6.175 ± 0.436
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski