Proteome-pI 2.0 - Proteome Isoelectric Point Database

Prevotella marshii DSM 16973 = JCM 13450

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; Prevotella marshii

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 2334 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|E0NTU5|E0NTU5_9BACT Uncharacterized protein OS=Prevotella marshii DSM 16973 = JCM 13450 OX=862515 GN=HMPREF0658_1598 PE=4 SV=1

MM1 pKa = 6.69MKK3 pKa = 10.15KK4 pKa = 9.76YY5 pKa = 10.7VCVPCGYY12 pKa = 10.12IYY14 pKa = 10.23DD15 pKa = 4.31PKK17 pKa = 11.0EE18 pKa = 3.82GDD20 pKa = 3.63PDD22 pKa = 3.79GGIAPGTAFEE32 pKa = 5.97DD33 pKa = 4.28IPDD36 pKa = 3.79DD37 pKa = 4.06WVCPICGVGKK47 pKa = 10.4DD48 pKa = 3.46EE49 pKa = 4.9FEE51 pKa = 4.39PVEE54 pKa = 4.21DD55 pKa = 3.8

Molecular weight: 6.01 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|E0NVA5|E0NVA5_9BACT Uncharacterized protein OS=Prevotella marshii DSM 16973 = JCM 13450 OX=862515 GN=HMPREF0658_2110 PE=4 SV=1

MM1 pKa = 7.81LILVLLSTQNGEE13 pKa = 3.74IFRR16 pKa = 11.84NRR18 pKa = 11.84SGSHH22 pKa = 5.05GRR24 pKa = 11.84TMTFLILSHH33 pKa = 6.27PRR35 pKa = 11.84IMTLNIHH42 pKa = 6.32NSMMSSSDD50 pKa = 3.08AEE52 pKa = 3.73RR53 pKa = 11.84FRR55 pKa = 11.84RR56 pKa = 11.84KK57 pKa = 10.07KK58 pKa = 8.44ITFASRR64 pKa = 11.84HH65 pKa = 4.75HH66 pKa = 7.26RR67 pKa = 11.84YY68 pKa = 10.2LLMKK72 pKa = 9.71RR73 pKa = 11.84TILALIFIFSCIAFTPLQVMGAPAIEE99 pKa = 4.72VIDD102 pKa = 4.8NDD104 pKa = 3.67VQTINISVSEE114 pKa = 4.35STLHH118 pKa = 4.76ITGGNGQTLHH128 pKa = 6.95IYY130 pKa = 9.86NVTGVRR136 pKa = 11.84VMSIRR141 pKa = 11.84VDD143 pKa = 3.63GMDD146 pKa = 4.47KK147 pKa = 10.3RR148 pKa = 11.84YY149 pKa = 10.17EE150 pKa = 3.99LNLPKK155 pKa = 10.32GCYY158 pKa = 8.11IVKK161 pKa = 9.98VGNVVRR167 pKa = 11.84KK168 pKa = 9.83ISLRR172 pKa = 3.63

Molecular weight: 19.49 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
2334	0	2334	734057	38	2519	314.5	35.44

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
7.658 ± 0.056	1.332 ± 0.019	5.55 ± 0.033	6.03 ± 0.055	4.407 ± 0.039	6.711 ± 0.049	2.291 ± 0.022	6.625 ± 0.047	6.314 ± 0.046	9.141 ± 0.05

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.857 ± 0.023	4.662 ± 0.046	3.76 ± 0.025	3.584 ± 0.033	5.364 ± 0.05	5.885 ± 0.04	6.167 ± 0.045	6.362 ± 0.038	1.114 ± 0.018	4.185 ± 0.036

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski