Squash leaf curl Yunnan virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8UYW2|Q8UYW2_9GEMI Transcriptional activator protein OS=Squash leaf curl Yunnan virus OX=222474 GN=AC2 PE=3 SV=1
MM1 pKa = 7.68PRR3 pKa = 11.84TNQFQVKK10 pKa = 9.47AKK12 pKa = 10.56NIFLTFPKK20 pKa = 10.37CPIPKK25 pKa = 9.54EE26 pKa = 3.96QMLEE30 pKa = 3.84LLQNISCPSDD40 pKa = 3.09KK41 pKa = 11.06LFIRR45 pKa = 11.84VSQEE49 pKa = 3.23KK50 pKa = 9.59HH51 pKa = 5.07QDD53 pKa = 3.08GSMHH57 pKa = 5.64IHH59 pKa = 7.06ALIQFKK65 pKa = 10.38GKK67 pKa = 9.81CQFRR71 pKa = 11.84NPRR74 pKa = 11.84HH75 pKa = 6.12FDD77 pKa = 3.1VTHH80 pKa = 7.25PNTSTQYY87 pKa = 10.45HH88 pKa = 7.05PNFQGAKK95 pKa = 9.08SSSDD99 pKa = 3.06VKK101 pKa = 11.23SYY103 pKa = 10.8IEE105 pKa = 4.55KK106 pKa = 10.86DD107 pKa = 2.8GDD109 pKa = 4.02YY110 pKa = 10.58IDD112 pKa = 4.14WGQFQIDD119 pKa = 3.44GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 3.91AAAEE136 pKa = 3.92ALNAGSAEE144 pKa = 3.84AALAIMRR151 pKa = 11.84EE152 pKa = 4.3KK153 pKa = 10.82LPKK156 pKa = 10.21DD157 pKa = 3.74YY158 pKa = 10.71IFQYY162 pKa = 11.22HH163 pKa = 6.51NIKK166 pKa = 10.69CNLDD170 pKa = 3.9RR171 pKa = 11.84IFTPPIEE178 pKa = 4.51EE179 pKa = 4.4YY180 pKa = 10.18ISPFSSSSFNQVPGEE195 pKa = 4.02LEE197 pKa = 3.53EE198 pKa = 4.27WACNNVLSEE207 pKa = 4.04AARR210 pKa = 11.84PFRR213 pKa = 11.84PISIVIEE220 pKa = 4.23GDD222 pKa = 3.2SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.39LSPKK249 pKa = 10.12VYY251 pKa = 11.11NNDD254 pKa = 2.08AWYY257 pKa = 10.88NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.6YY287 pKa = 10.57GKK289 pKa = 9.12PVQIKK294 pKa = 10.41GGIPTIFLCNPGPNSSYY311 pKa = 11.02KK312 pKa = 10.47EE313 pKa = 3.86YY314 pKa = 11.18LEE316 pKa = 4.23EE317 pKa = 4.3EE318 pKa = 4.42KK319 pKa = 11.19NSALRR324 pKa = 11.84NWAIKK329 pKa = 10.12NAIFVTLEE337 pKa = 3.94GPLYY341 pKa = 10.68SDD343 pKa = 4.67TYY345 pKa = 11.2QGATPNSEE353 pKa = 4.24EE354 pKa = 4.56DD355 pKa = 3.55NQTTEE360 pKa = 3.88SS361 pKa = 3.56

Molecular weight: 41.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8UYW5|Q8UYW5_9GEMI Protein V2 OS=Squash leaf curl Yunnan virus OX=222474 GN=AV2 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.38RR4 pKa = 11.84PADD7 pKa = 3.55ILISTPVSKK16 pKa = 10.58VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.0SPYY27 pKa = 10.03NSRR30 pKa = 11.84AAVPTVRR37 pKa = 11.84VTKK40 pKa = 10.33GQIWKK45 pKa = 9.28NRR47 pKa = 11.84PAYY50 pKa = 9.67RR51 pKa = 11.84KK52 pKa = 8.23PKK54 pKa = 9.08FYY56 pKa = 11.04RR57 pKa = 11.84MYY59 pKa = 10.55RR60 pKa = 11.84SPDD63 pKa = 3.29VPKK66 pKa = 10.81GCEE69 pKa = 4.39GPCKK73 pKa = 10.37VQSFDD78 pKa = 3.26AKK80 pKa = 11.06NDD82 pKa = 3.27IGHH85 pKa = 6.11MGKK88 pKa = 10.22VICLSDD94 pKa = 3.33VTRR97 pKa = 11.84GIGLTHH103 pKa = 6.79RR104 pKa = 11.84VDD106 pKa = 3.86KK107 pKa = 10.77RR108 pKa = 11.84FCVKK112 pKa = 10.17SLYY115 pKa = 10.31FVGKK119 pKa = 9.14IWMDD123 pKa = 3.34EE124 pKa = 4.02NIKK127 pKa = 10.41VKK129 pKa = 10.66NHH131 pKa = 5.45TNTVLFWIVRR141 pKa = 11.84DD142 pKa = 3.85RR143 pKa = 11.84RR144 pKa = 11.84PTGTPYY150 pKa = 11.16DD151 pKa = 3.75FQQVFNVYY159 pKa = 10.67DD160 pKa = 4.09NEE162 pKa = 4.37PSTATVKK169 pKa = 10.59NDD171 pKa = 3.0QRR173 pKa = 11.84DD174 pKa = 3.37RR175 pKa = 11.84FQVIRR180 pKa = 11.84RR181 pKa = 11.84FQATVTGGQYY191 pKa = 10.18AAKK194 pKa = 8.87EE195 pKa = 3.58QAIIRR200 pKa = 11.84KK201 pKa = 8.88FYY203 pKa = 10.48RR204 pKa = 11.84VNNYY208 pKa = 7.79VVYY211 pKa = 10.32NHH213 pKa = 6.0QEE215 pKa = 3.43AGKK218 pKa = 10.41YY219 pKa = 8.39EE220 pKa = 4.09NHH222 pKa = 6.52TEE224 pKa = 3.99NALLLYY230 pKa = 7.29MACTHH235 pKa = 7.07ASNPVYY241 pKa = 9.99ATLKK245 pKa = 8.67VRR247 pKa = 11.84SYY249 pKa = 10.98FYY251 pKa = 11.25DD252 pKa = 3.13SVTNN256 pKa = 3.98

Molecular weight: 29.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1092

94

361

182.0

20.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.945 ± 0.861

2.381 ± 0.403

4.945 ± 0.311

4.945 ± 0.792

4.304 ± 0.441

4.487 ± 0.576

3.297 ± 0.551

6.41 ± 0.758

5.861 ± 0.695

6.868 ± 1.097

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.198 ± 0.617

6.502 ± 0.508

5.495 ± 0.711

5.403 ± 0.544

6.777 ± 1.13

8.059 ± 1.186

6.044 ± 0.698

5.861 ± 1.284

1.282 ± 0.234

3.938 ± 0.716
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski