Lactococcus phage proPhi1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649V220|A0A649V220_9CAUD DNA repair/replication protein OS=Lactococcus phage proPhi1 OX=2656509 PE=4 SV=1
MM1 pKa = 7.84SEE3 pKa = 4.33LEE5 pKa = 4.07KK6 pKa = 10.09TAQQIAAEE14 pKa = 4.04ALNFKK19 pKa = 10.47SKK21 pKa = 10.61AYY23 pKa = 8.65YY24 pKa = 9.83TIEE27 pKa = 4.06QQNEE31 pKa = 3.88TIAANRR37 pKa = 11.84KK38 pKa = 9.19AFMKK42 pKa = 10.0ILSISSLTIPKK53 pKa = 10.16SIADD57 pKa = 3.69EE58 pKa = 4.22LEE60 pKa = 3.93EE61 pKa = 4.69EE62 pKa = 4.16IEE64 pKa = 4.03EE65 pKa = 4.87AYY67 pKa = 10.89SSDD70 pKa = 3.88YY71 pKa = 10.19IRR73 pKa = 11.84SYY75 pKa = 11.54SDD77 pKa = 2.93VGACMEE83 pKa = 4.73VITDD87 pKa = 3.89GLDD90 pKa = 3.41EE91 pKa = 4.37EE92 pKa = 4.75SEE94 pKa = 4.46LYY96 pKa = 10.93KK97 pKa = 11.2FMFPDD102 pKa = 4.72DD103 pKa = 4.24ALLGCSHH110 pKa = 7.0RR111 pKa = 11.84NIIYY115 pKa = 10.39LYY117 pKa = 10.78LVDD120 pKa = 4.27NDD122 pKa = 3.92LVKK125 pKa = 11.0VGEE128 pKa = 4.37GG129 pKa = 3.21

Molecular weight: 14.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649V0Z2|A0A649V0Z2_9CAUD HeH domain-containing protein OS=Lactococcus phage proPhi1 OX=2656509 PE=4 SV=1
MM1 pKa = 7.68SYY3 pKa = 10.37FGSLAQLVANRR14 pKa = 11.84SVDD17 pKa = 3.12GSSPARR23 pKa = 11.84TIKK26 pKa = 10.0KK27 pKa = 9.47RR28 pKa = 11.84RR29 pKa = 11.84VKK31 pKa = 10.64KK32 pKa = 10.6RR33 pKa = 11.84GGNLANSFIIAFF45 pKa = 4.12

Molecular weight: 4.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12725

42

918

205.2

23.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.483 ± 0.286

0.589 ± 0.092

6.106 ± 0.207

7.348 ± 0.512

4.291 ± 0.186

6.2 ± 0.366

1.171 ± 0.138

6.64 ± 0.231

9.242 ± 0.464

8.259 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.436 ± 0.132

6.114 ± 0.27

2.845 ± 0.161

4.173 ± 0.192

3.568 ± 0.289

6.743 ± 0.332

6.193 ± 0.404

6.067 ± 0.28

1.548 ± 0.201

3.984 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski