Proteome-pI 2.0 - Proteome Isoelectric Point Database

Firmicutes bacterium ASF500

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; unclassified Firmicutes sensu stricto

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 3472 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|V2YG02|V2YG02_9FIRM Uncharacterized protein OS=Firmicutes bacterium ASF500 OX=1378168 GN=N510_01742 PE=4 SV=1

MM1 pKa = 7.6KK2 pKa = 10.51KK3 pKa = 10.41LLLTALIASMLIVPLGNTALAGHH26 pKa = 6.41EE27 pKa = 4.16EE28 pKa = 4.21VAPPVSAQDD37 pKa = 3.83FVPGDD42 pKa = 3.7APAGNAAPEE51 pKa = 3.99AMLPAVHH58 pKa = 6.42GLVLAMLNHH67 pKa = 7.0DD68 pKa = 3.67MDD70 pKa = 4.05EE71 pKa = 4.33FDD73 pKa = 3.97RR74 pKa = 11.84TNSALAWEE82 pKa = 4.75SLYY85 pKa = 11.78NMLSLYY91 pKa = 11.02GEE93 pKa = 4.28LDD95 pKa = 3.67SRR97 pKa = 11.84CDD99 pKa = 3.47EE100 pKa = 4.66SNGEE104 pKa = 4.11LFLLEE109 pKa = 4.1EE110 pKa = 4.25TVRR113 pKa = 11.84DD114 pKa = 3.72YY115 pKa = 11.95ASALDD120 pKa = 3.94LQLDD124 pKa = 3.92EE125 pKa = 5.85LGPLPEE131 pKa = 4.58EE132 pKa = 3.56LWDD135 pKa = 4.72RR136 pKa = 11.84INYY139 pKa = 10.0DD140 pKa = 3.51NVSQGYY146 pKa = 8.62VLVCGDD152 pKa = 4.16TGPVQIQMDD161 pKa = 4.4SLQNGQDD168 pKa = 3.79GITLSGSLIYY178 pKa = 10.3LASGEE183 pKa = 4.26VLASFQAGLSATDD196 pKa = 3.25SMFGFAIDD204 pKa = 3.83TLTVNAA210 pKa = 5.23

Molecular weight: 22.43 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|V2ZKM8|V2ZKM8_9FIRM Uncharacterized protein OS=Firmicutes bacterium ASF500 OX=1378168 GN=N510_00449 PE=4 SV=1

MM1 pKa = 7.45LRR3 pKa = 11.84TYY5 pKa = 9.8QPKK8 pKa = 9.6KK9 pKa = 7.95RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.69EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 10.07RR21 pKa = 11.84MATRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 8.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84VRR41 pKa = 11.84LTHH44 pKa = 6.42

Molecular weight: 5.42 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
3472	0	3472	1032909	22	5424	297.5	32.96

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
9.314 ± 0.049	1.573 ± 0.02	5.565 ± 0.036	7.009 ± 0.051	3.767 ± 0.03	7.912 ± 0.048	1.75 ± 0.019	5.349 ± 0.038	4.854 ± 0.037	9.957 ± 0.065

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.695 ± 0.023	3.291 ± 0.031	4.378 ± 0.032	3.74 ± 0.026	5.968 ± 0.059	5.674 ± 0.038	5.519 ± 0.059	6.972 ± 0.04	1.173 ± 0.018	3.541 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski