Beet soil-borne virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Pomovirus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q65728|Q65728_9VIRU 22K protein OS=Beet soil-borne virus OX=46436 PE=4 SV=1
MM1 pKa = 8.06DD2 pKa = 5.46PPAIIHH8 pKa = 5.92SQNCCCPDD16 pKa = 4.14CSWQPSCTHH25 pKa = 6.44TSNTEE30 pKa = 3.88SLNARR35 pKa = 11.84SPVEE39 pKa = 3.79EE40 pKa = 4.01NVRR43 pKa = 11.84MVKK46 pKa = 10.3GYY48 pKa = 8.41STDD51 pKa = 3.64YY52 pKa = 11.34VIMCVIVSVSLGFAIAAYY70 pKa = 8.95FYY72 pKa = 11.14SSGGHH77 pKa = 5.96YY78 pKa = 10.84DD79 pKa = 3.1PDD81 pKa = 3.51AAIFKK86 pKa = 10.25QDD88 pKa = 3.58LNEE91 pKa = 3.9VQINFGKK98 pKa = 10.24HH99 pKa = 5.58PIDD102 pKa = 3.83PKK104 pKa = 10.59VIEE107 pKa = 5.1AVHH110 pKa = 5.77HH111 pKa = 5.12WQRR114 pKa = 11.84APFGRR119 pKa = 11.84FQGEE123 pKa = 4.29SVDD126 pKa = 3.43VSKK129 pKa = 11.51NFFEE133 pKa = 5.79DD134 pKa = 4.42SVDD137 pKa = 3.57EE138 pKa = 4.61DD139 pKa = 4.34DD140 pKa = 3.95EE141 pKa = 4.53EE142 pKa = 4.6EE143 pKa = 3.87RR144 pKa = 11.84AEE146 pKa = 4.17VNKK149 pKa = 10.99EE150 pKa = 3.61EE151 pKa = 4.16VTKK154 pKa = 10.72KK155 pKa = 9.42KK156 pKa = 10.94SKK158 pKa = 10.38VAVCNRR164 pKa = 11.84LCSIRR169 pKa = 11.84NSLLRR174 pKa = 11.84LFWVITLIIIVVKK187 pKa = 10.74CRR189 pKa = 11.84FF190 pKa = 3.24

Molecular weight: 21.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q65728|Q65728_9VIRU 22K protein OS=Beet soil-borne virus OX=46436 PE=4 SV=1
MM1 pKa = 7.19VRR3 pKa = 11.84TNEE6 pKa = 3.49IGARR10 pKa = 11.84PNKK13 pKa = 9.13YY14 pKa = 7.88WPIVVGVFAIALFSFLTITNQKK36 pKa = 9.73HH37 pKa = 4.12ATEE40 pKa = 4.67SGDD43 pKa = 3.87NIHH46 pKa = 6.77KK47 pKa = 10.2FSNGGKK53 pKa = 9.42FQDD56 pKa = 3.56GNKK59 pKa = 9.16RR60 pKa = 11.84VHH62 pKa = 6.03YY63 pKa = 10.22NKK65 pKa = 10.32NNPRR69 pKa = 11.84AYY71 pKa = 10.44NGSSSNNTFSKK82 pKa = 10.89LLLPGLFLAAIMYY95 pKa = 10.16AYY97 pKa = 9.84VQYY100 pKa = 10.7RR101 pKa = 11.84KK102 pKa = 8.75PQCTVTCRR110 pKa = 11.84GEE112 pKa = 4.18CAHH115 pKa = 6.8GG116 pKa = 3.49

Molecular weight: 12.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

4876

116

1778

696.6

79.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.44 ± 0.467

1.887 ± 0.258

7.055 ± 0.234

7.424 ± 0.282

4.573 ± 0.193

5.435 ± 0.386

1.907 ± 0.301

5.558 ± 0.221

7.075 ± 0.441

8.306 ± 0.451

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.461 ± 0.295

4.82 ± 0.223

2.728 ± 0.17

2.871 ± 0.222

6.44 ± 0.48

7.568 ± 0.39

5.476 ± 0.263

7.465 ± 0.28

1.025 ± 0.174

3.445 ± 0.345
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski