Ponticoccus marisrubri

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Ponticoccus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4264 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0W7WN60|A0A0W7WN60_9RHOB Uncharacterized protein OS=Ponticoccus marisrubri OX=1685382 GN=AVJ23_05475 PE=4 SV=1
MM1 pKa = 7.53KK2 pKa = 10.44KK3 pKa = 10.44LPLMLAAGAVAAPMAAQAQSDD24 pKa = 4.17GCGEE28 pKa = 4.03VSITEE33 pKa = 4.21MNWASSQVVTSVASFLMQQGYY54 pKa = 8.66GCEE57 pKa = 4.19VNVVSSDD64 pKa = 3.84TIPAITSVAEE74 pKa = 3.92NGEE77 pKa = 3.98PDD79 pKa = 3.41IVTEE83 pKa = 4.39LWLNSTGDD91 pKa = 3.14TYY93 pKa = 11.25TQLKK97 pKa = 9.12EE98 pKa = 3.73AGKK101 pKa = 9.91VVEE104 pKa = 4.7AAPVLDD110 pKa = 4.2PGGVEE115 pKa = 4.41GWWIPTYY122 pKa = 10.38LAEE125 pKa = 4.26EE126 pKa = 4.42HH127 pKa = 7.14PEE129 pKa = 3.8LTTIEE134 pKa = 4.98GILANPEE141 pKa = 3.94LVDD144 pKa = 3.63GRR146 pKa = 11.84FYY148 pKa = 11.26NCPEE152 pKa = 3.46GWGCRR157 pKa = 11.84VVSDD161 pKa = 4.23NLSAALDD168 pKa = 3.78LEE170 pKa = 4.82GNGIEE175 pKa = 4.39VFNSGSGQVLATSMADD191 pKa = 2.78AVLNEE196 pKa = 4.4EE197 pKa = 4.31PWFGYY202 pKa = 9.75YY203 pKa = 9.38WGPTVPLGKK212 pKa = 10.2YY213 pKa = 10.82DD214 pKa = 3.45MTKK217 pKa = 9.95IDD219 pKa = 3.7LGEE222 pKa = 4.15VNEE225 pKa = 5.02EE226 pKa = 3.55IHH228 pKa = 6.63NANQNADD235 pKa = 3.35NDD237 pKa = 4.04NPGVSDD243 pKa = 5.14FPAAPIVTATTTTFQEE259 pKa = 4.4SNPEE263 pKa = 3.9VFALLQNMTFKK274 pKa = 10.51TSDD277 pKa = 3.42MSSVLAWKK285 pKa = 10.35DD286 pKa = 3.44EE287 pKa = 4.34NNASAEE293 pKa = 4.1EE294 pKa = 3.87AAVYY298 pKa = 10.79YY299 pKa = 7.79LTNYY303 pKa = 8.17QDD305 pKa = 4.32EE306 pKa = 4.53WAGWLNDD313 pKa = 3.55AARR316 pKa = 11.84DD317 pKa = 3.88NLSALLSQQ325 pKa = 4.43

Molecular weight: 34.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0W7WPK4|A0A0W7WPK4_9RHOB 50S ribosomal protein L30 OS=Ponticoccus marisrubri OX=1685382 GN=rpmD PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4264

0

4264

1336786

41

2158

313.5

33.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.093 ± 0.061

0.899 ± 0.012

6.137 ± 0.041

6.072 ± 0.046

3.57 ± 0.022

8.934 ± 0.041

2.079 ± 0.019

4.657 ± 0.03

2.468 ± 0.032

10.299 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.688 ± 0.015

2.249 ± 0.024

5.438 ± 0.032

3.177 ± 0.019

7.346 ± 0.044

4.664 ± 0.026

5.379 ± 0.028

7.259 ± 0.033

1.449 ± 0.016

2.14 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski