Escherichia phage EC6098

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Gokushovirinae; Enterogokushovirus; Enterogokushovirus EC6098

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G9L7S3|A0A6G9L7S3_9VIRU DNA pilot protein OS=Escherichia phage EC6098 OX=2720215 GN=vp2 PE=4 SV=1
MM1 pKa = 7.2AHH3 pKa = 6.68SDD5 pKa = 3.51AVYY8 pKa = 10.43VIHH11 pKa = 7.9DD12 pKa = 3.89KK13 pKa = 11.19LINADD18 pKa = 3.06VFMFMAQHH26 pKa = 6.62DD27 pKa = 5.14GIAKK31 pKa = 8.81GHH33 pKa = 6.28LHH35 pKa = 6.69AFCNRR40 pKa = 11.84MQRR43 pKa = 11.84TDD45 pKa = 3.7FSLHH49 pKa = 6.2RR50 pKa = 11.84VTAYY54 pKa = 10.7DD55 pKa = 3.77FADD58 pKa = 3.61QQLVPVDD65 pKa = 3.2IDD67 pKa = 3.22RR68 pKa = 11.84VYY70 pKa = 11.14VCEE73 pKa = 4.06ATPAFLKK80 pKa = 10.65DD81 pKa = 3.46SEE83 pKa = 4.43DD84 pKa = 3.9AYY86 pKa = 10.28IEE88 pKa = 4.08AA89 pKa = 5.19

Molecular weight: 10.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G9L952|A0A6G9L952_9VIRU Replication initiation protein OS=Escherichia phage EC6098 OX=2720215 GN=vp4 PE=4 SV=1
MM1 pKa = 7.14ARR3 pKa = 11.84SRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84MSKK10 pKa = 9.3RR11 pKa = 11.84SSRR14 pKa = 11.84RR15 pKa = 11.84SFRR18 pKa = 11.84KK19 pKa = 8.84YY20 pKa = 11.0AKK22 pKa = 6.48THH24 pKa = 5.11KK25 pKa = 10.11RR26 pKa = 11.84NFKK29 pKa = 9.63ARR31 pKa = 11.84SMRR34 pKa = 11.84GGIRR38 pKa = 11.84LL39 pKa = 3.44

Molecular weight: 4.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1421

39

566

236.8

26.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.797 ± 1.539

1.267 ± 0.709

5.771 ± 0.702

4.504 ± 0.755

5.489 ± 0.714

7.46 ± 0.936

2.252 ± 0.673

4.926 ± 0.199

4.574 ± 0.612

6.967 ± 0.75

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.533 ± 0.671

4.926 ± 0.62

5.348 ± 1.196

5.137 ± 1.333

7.389 ± 1.054

7.811 ± 0.767

4.082 ± 0.433

5.771 ± 0.425

0.915 ± 0.261

4.082 ± 0.599
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski