Limeum africanum associated virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; unclassified Geminiviridae

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I8B2M7|A0A2I8B2M7_9GEMI Capsid protein OS=Limeum africanum associated virus OX=2093276 PE=3 SV=1
MM1 pKa = 7.4APYY4 pKa = 9.99RR5 pKa = 11.84VRR7 pKa = 11.84DD8 pKa = 4.07FPDD11 pKa = 3.96TYY13 pKa = 11.12PKK15 pKa = 10.43FLAACTRR22 pKa = 11.84CLLQWNKK29 pKa = 10.47YY30 pKa = 9.05CILGIQLEE38 pKa = 4.36SGEE41 pKa = 4.48LDD43 pKa = 3.53LEE45 pKa = 4.23EE46 pKa = 4.59EE47 pKa = 4.01EE48 pKa = 6.44AYY50 pKa = 10.89LQFKK54 pKa = 10.83KK55 pKa = 10.21EE56 pKa = 4.02AQKK59 pKa = 11.15LLQKK63 pKa = 10.36KK64 pKa = 9.83VNFHH68 pKa = 5.94TKK70 pKa = 10.29CEE72 pKa = 4.19LYY74 pKa = 11.07LEE76 pKa = 4.61LKK78 pKa = 10.02KK79 pKa = 10.83KK80 pKa = 10.38YY81 pKa = 10.39GPSEE85 pKa = 4.1EE86 pKa = 5.34EE87 pKa = 3.67KK88 pKa = 11.24VEE90 pKa = 4.15WNDD93 pKa = 3.19QLAEE97 pKa = 4.15SEE99 pKa = 4.16EE100 pKa = 4.38DD101 pKa = 3.23SWEE104 pKa = 4.55DD105 pKa = 3.36GTKK108 pKa = 9.52EE109 pKa = 3.71VSMEE113 pKa = 4.06EE114 pKa = 4.13TCEE117 pKa = 3.95EE118 pKa = 4.25GQGCQTEE125 pKa = 4.42DD126 pKa = 3.23VRR128 pKa = 5.67

Molecular weight: 15.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I8B2Q9|A0A2I8B2Q9_9GEMI V3 OS=Limeum africanum associated virus OX=2093276 PE=4 SV=1
MM1 pKa = 7.2AQVKK5 pKa = 8.87KK6 pKa = 10.5RR7 pKa = 11.84KK8 pKa = 8.05WSGMTSWPNRR18 pKa = 11.84KK19 pKa = 8.22RR20 pKa = 11.84TAGKK24 pKa = 7.73TAQKK28 pKa = 10.12KK29 pKa = 6.32YY30 pKa = 6.96QWRR33 pKa = 11.84KK34 pKa = 8.36PVRR37 pKa = 11.84KK38 pKa = 10.0DD39 pKa = 3.02KK40 pKa = 10.98AVKK43 pKa = 10.31LKK45 pKa = 10.36MYY47 pKa = 10.55DD48 pKa = 3.47DD49 pKa = 3.88TFGNQGIGSAVSNDD63 pKa = 3.0GMITMVNNYY72 pKa = 8.22VQGIGDD78 pKa = 3.94SQRR81 pKa = 11.84SSNTTVTRR89 pKa = 11.84HH90 pKa = 5.89LKK92 pKa = 10.39FDD94 pKa = 3.68MALMASSGFWQAPNYY109 pKa = 5.3MTQYY113 pKa = 10.39HH114 pKa = 6.58WLLVDD119 pKa = 4.54RR120 pKa = 11.84DD121 pKa = 3.9ATGPFPDD128 pKa = 4.01KK129 pKa = 10.85LSTIFDD135 pKa = 3.72IPNNGQACPSTYY147 pKa = 10.09RR148 pKa = 11.84IRR150 pKa = 11.84RR151 pKa = 11.84DD152 pKa = 3.27CNEE155 pKa = 3.48RR156 pKa = 11.84FIVKK160 pKa = 10.08RR161 pKa = 11.84KK162 pKa = 4.84WTTHH166 pKa = 4.88LMSTGTDD173 pKa = 3.25YY174 pKa = 11.34GVKK177 pKa = 8.32QTYY180 pKa = 8.8KK181 pKa = 10.52APSMPNYY188 pKa = 9.16KK189 pKa = 10.1KK190 pKa = 10.94AMNHH194 pKa = 5.13NFRR197 pKa = 11.84NINVKK202 pKa = 7.16TTWKK206 pKa = 9.17DD207 pKa = 3.2TGGGKK212 pKa = 10.11YY213 pKa = 10.33EE214 pKa = 4.09DD215 pKa = 3.65VKK217 pKa = 11.35EE218 pKa = 3.97NALLYY223 pKa = 10.73VVVNDD228 pKa = 4.55NVDD231 pKa = 3.16NTNIYY236 pKa = 10.15AQLFGNIRR244 pKa = 11.84AYY246 pKa = 10.32FHH248 pKa = 7.04

Molecular weight: 28.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1053

90

333

210.6

24.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.463 ± 0.467

2.089 ± 0.468

5.793 ± 0.428

6.268 ± 2.057

4.084 ± 0.235

5.603 ± 0.708

2.754 ± 0.436

5.223 ± 0.771

6.553 ± 1.292

7.597 ± 0.991

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.994 ± 0.717

5.888 ± 1.036

5.793 ± 0.96

5.508 ± 0.333

5.983 ± 0.438

5.983 ± 0.896

6.078 ± 0.641

5.318 ± 0.51

2.469 ± 0.096

4.558 ± 0.508
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski