Human metapneumovirus (strain CAN97-83) (HMPV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Pneumoviridae; Metapneumovirus; Human metapneumovirus

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q8B9Q8|PHOSP_HMPVC Phosphoprotein OS=Human metapneumovirus (strain CAN97-83) OX=694067 GN=P PE=1 SV=1
MM1 pKa = 7.55SFPEE5 pKa = 4.49GKK7 pKa = 10.16DD8 pKa = 2.84ILFMGNEE15 pKa = 3.85AAKK18 pKa = 10.34LAEE21 pKa = 4.55AFQKK25 pKa = 10.38SLRR28 pKa = 11.84KK29 pKa = 9.13PSHH32 pKa = 6.41KK33 pKa = 9.83RR34 pKa = 11.84SQSIIGEE41 pKa = 4.26KK42 pKa = 10.47VNTVSEE48 pKa = 4.29TLEE51 pKa = 4.38LPTISRR57 pKa = 11.84PTKK60 pKa = 8.75PTILSEE66 pKa = 4.38PKK68 pKa = 10.39LAWTDD73 pKa = 2.87KK74 pKa = 10.85GGAIKK79 pKa = 9.81TEE81 pKa = 3.85AKK83 pKa = 8.85QTIKK87 pKa = 10.96VMDD90 pKa = 5.18PIEE93 pKa = 4.26EE94 pKa = 4.2EE95 pKa = 4.03EE96 pKa = 4.18FTEE99 pKa = 4.89KK100 pKa = 10.43RR101 pKa = 11.84VLPSSDD107 pKa = 2.6GKK109 pKa = 9.18TPAEE113 pKa = 4.28KK114 pKa = 10.33KK115 pKa = 10.41LKK117 pKa = 10.37PSTNTKK123 pKa = 9.95KK124 pKa = 10.56KK125 pKa = 10.91VSFTPNEE132 pKa = 3.81PGKK135 pKa = 8.74YY136 pKa = 8.55TKK138 pKa = 10.59LEE140 pKa = 3.97KK141 pKa = 10.45DD142 pKa = 3.58ALDD145 pKa = 3.95LLSDD149 pKa = 3.95NEE151 pKa = 4.16EE152 pKa = 3.86EE153 pKa = 4.39DD154 pKa = 4.05AEE156 pKa = 4.49SSILTFEE163 pKa = 4.34EE164 pKa = 4.82RR165 pKa = 11.84DD166 pKa = 3.58TSSLSIEE173 pKa = 4.18ARR175 pKa = 11.84LEE177 pKa = 4.23SIEE180 pKa = 4.71EE181 pKa = 4.18KK182 pKa = 10.85LSMILGLLRR191 pKa = 11.84TLNIATAGPTAARR204 pKa = 11.84DD205 pKa = 4.16GIRR208 pKa = 11.84DD209 pKa = 3.43AMIGIRR215 pKa = 11.84EE216 pKa = 4.13EE217 pKa = 4.64LIADD221 pKa = 4.3IIKK224 pKa = 9.71EE225 pKa = 4.32AKK227 pKa = 9.86GKK229 pKa = 9.21AAEE232 pKa = 4.1MMEE235 pKa = 4.19EE236 pKa = 4.42EE237 pKa = 4.33MNQRR241 pKa = 11.84TKK243 pKa = 10.55IGNGSVKK250 pKa = 9.54LTEE253 pKa = 4.33KK254 pKa = 10.76AKK256 pKa = 9.89EE257 pKa = 3.84LNKK260 pKa = 10.09IVEE263 pKa = 4.67DD264 pKa = 4.06EE265 pKa = 4.41STSGEE270 pKa = 4.21SEE272 pKa = 4.07EE273 pKa = 4.47EE274 pKa = 4.15EE275 pKa = 4.18EE276 pKa = 5.87LKK278 pKa = 10.09DD279 pKa = 3.58TQEE282 pKa = 4.17NNQEE286 pKa = 3.77DD287 pKa = 5.25DD288 pKa = 3.43IYY290 pKa = 11.19QLIMM294 pKa = 4.27

Molecular weight: 32.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q6WB95|SH_HMPVC Small hydrophobic protein OS=Human metapneumovirus (strain CAN97-83) OX=694067 GN=SH PE=1 SV=1
MM1 pKa = 7.13EE2 pKa = 4.63VKK4 pKa = 10.43VEE6 pKa = 4.26NIRR9 pKa = 11.84AIDD12 pKa = 3.62MLKK15 pKa = 10.53ARR17 pKa = 11.84VKK19 pKa = 10.9NRR21 pKa = 11.84VARR24 pKa = 11.84SKK26 pKa = 10.7CFKK29 pKa = 10.02NASLILIGITTLSIALNIYY48 pKa = 10.04LIINYY53 pKa = 7.58TIQKK57 pKa = 7.96TSSEE61 pKa = 4.63SEE63 pKa = 4.05HH64 pKa = 6.38HH65 pKa = 6.38TSSPPTEE72 pKa = 4.39SNKK75 pKa = 9.88EE76 pKa = 3.7ASTISTDD83 pKa = 4.23NPDD86 pKa = 3.43INPNSQHH93 pKa = 6.32PTQQSTEE100 pKa = 3.98NPTLNPAASVSPSEE114 pKa = 4.44TEE116 pKa = 3.86PASTPDD122 pKa = 3.14TTNRR126 pKa = 11.84LSSVDD131 pKa = 3.43RR132 pKa = 11.84STAQPSEE139 pKa = 4.29SRR141 pKa = 11.84TKK143 pKa = 10.09TKK145 pKa = 9.36PTVHH149 pKa = 6.0TRR151 pKa = 11.84NNPSTASSTQSPPRR165 pKa = 11.84ATTKK169 pKa = 10.48AIRR172 pKa = 11.84RR173 pKa = 11.84ATTFRR178 pKa = 11.84MSSTGKK184 pKa = 10.35RR185 pKa = 11.84PTTTSVQSDD194 pKa = 3.61SSTTTQNHH202 pKa = 6.17EE203 pKa = 4.37EE204 pKa = 4.16TGSANPQASVSTMQNN219 pKa = 2.73

Molecular weight: 23.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

4142

71

2005

460.2

51.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.674 ± 1.039

1.835 ± 0.321

4.732 ± 0.292

6.663 ± 0.883

3.356 ± 0.612

4.708 ± 0.55

1.787 ± 0.256

7.798 ± 0.382

7.846 ± 0.606

10.092 ± 0.954

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.487 ± 0.277

5.529 ± 0.464

3.597 ± 0.439

3.573 ± 0.295

4.491 ± 0.479

8.788 ± 0.839

6.808 ± 1.096

5.963 ± 0.589

1.062 ± 0.243

3.211 ± 0.395
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski