Vibrio phage vB_VpaS_MAR10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Mardecavirus; Vibrio virus MAR10

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 107 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7RVV2|K7RVV2_9CAUD Uncharacterized protein OS=Vibrio phage vB_VpaS_MAR10 OX=1229755 GN=MAR10_100 PE=4 SV=1
MM1 pKa = 8.04DD2 pKa = 5.31LQTSIYY8 pKa = 10.41AADD11 pKa = 3.72NRR13 pKa = 11.84YY14 pKa = 9.89HH15 pKa = 6.09YY16 pKa = 11.02ADD18 pKa = 4.63RR19 pKa = 11.84YY20 pKa = 10.08DD21 pKa = 3.62EE22 pKa = 5.4HH23 pKa = 6.74GTLHH27 pKa = 7.45DD28 pKa = 4.78LACEE32 pKa = 4.02YY33 pKa = 11.1ADD35 pKa = 4.43KK36 pKa = 11.21YY37 pKa = 8.83QAEE40 pKa = 4.32YY41 pKa = 10.79LEE43 pKa = 4.52NVNDD47 pKa = 5.72DD48 pKa = 4.48KK49 pKa = 12.03DD50 pKa = 4.52HH51 pKa = 8.06DD52 pKa = 4.61DD53 pKa = 4.27ADD55 pKa = 4.11QLAHH59 pKa = 7.32DD60 pKa = 4.46YY61 pKa = 11.22ARR63 pKa = 11.84EE64 pKa = 3.91TVLDD68 pKa = 4.15GMCWFSVRR76 pKa = 11.84FFAITYY82 pKa = 7.75PDD84 pKa = 4.89HH85 pKa = 6.57ILNRR89 pKa = 11.84TPHH92 pKa = 6.44DD93 pKa = 3.54TLGADD98 pKa = 3.8TPDD101 pKa = 3.11GLIRR105 pKa = 11.84RR106 pKa = 11.84NIEE109 pKa = 3.98EE110 pKa = 4.43VVTAAAATMIEE121 pKa = 4.26EE122 pKa = 3.97MRR124 pKa = 11.84EE125 pKa = 3.9RR126 pKa = 11.84GDD128 pKa = 3.9LDD130 pKa = 3.82RR131 pKa = 11.84DD132 pKa = 3.61EE133 pKa = 4.75

Molecular weight: 15.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7R6L0|K7R6L0_9CAUD Uncharacterized protein OS=Vibrio phage vB_VpaS_MAR10 OX=1229755 GN=MAR10_096 PE=4 SV=1
MM1 pKa = 7.23RR2 pKa = 11.84TKK4 pKa = 9.72FTNDD8 pKa = 2.61EE9 pKa = 4.14LDD11 pKa = 4.2RR12 pKa = 11.84IEE14 pKa = 4.75AATGYY19 pKa = 10.13RR20 pKa = 11.84IRR22 pKa = 11.84LRR24 pKa = 11.84SEE26 pKa = 3.52HH27 pKa = 7.0PYY29 pKa = 10.09YY30 pKa = 10.52SAYY33 pKa = 10.67LKK35 pKa = 10.72ARR37 pKa = 11.84QRR39 pKa = 11.84CLDD42 pKa = 4.49PDD44 pKa = 3.76HH45 pKa = 7.3EE46 pKa = 4.88SYY48 pKa = 10.34PWYY51 pKa = 10.29GGRR54 pKa = 11.84GLTFGFNGAVGFTQYY69 pKa = 10.85CEE71 pKa = 3.84EE72 pKa = 4.81LGRR75 pKa = 11.84KK76 pKa = 8.54PSPKK80 pKa = 8.65HH81 pKa = 4.41TVDD84 pKa = 4.95RR85 pKa = 11.84RR86 pKa = 11.84DD87 pKa = 3.07NDD89 pKa = 3.37LGYY92 pKa = 11.08VPGNIRR98 pKa = 11.84WASPAQQGANQRR110 pKa = 11.84KK111 pKa = 5.62TKK113 pKa = 9.97KK114 pKa = 9.51YY115 pKa = 7.85PYY117 pKa = 8.45KK118 pKa = 10.58GRR120 pKa = 11.84MMTARR125 pKa = 11.84RR126 pKa = 11.84ISKK129 pKa = 8.33ITGLSKK135 pKa = 10.87AVIADD140 pKa = 3.69RR141 pKa = 11.84WRR143 pKa = 11.84RR144 pKa = 11.84GEE146 pKa = 4.94RR147 pKa = 11.84GDD149 pKa = 3.05RR150 pKa = 11.84WLRR153 pKa = 11.84PAQRR157 pKa = 11.84AA158 pKa = 3.31

Molecular weight: 18.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

107

0

107

24022

33

948

224.5

25.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.826 ± 0.345

1.232 ± 0.108

6.527 ± 0.216

7.006 ± 0.299

3.996 ± 0.135

7.152 ± 0.186

2.061 ± 0.117

5.237 ± 0.127

6.573 ± 0.242

8.376 ± 0.166

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.606 ± 0.141

4.271 ± 0.171

4.025 ± 0.172

3.455 ± 0.152

5.674 ± 0.237

5.857 ± 0.191

6.132 ± 0.224

6.969 ± 0.198

1.524 ± 0.109

3.501 ± 0.141
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski