Escherichia phage vB_EcoS Sa179lw

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1MXD3|A0A2P1MXD3_9CAUD Putative type III restriction endonuclease OS=Escherichia phage vB_EcoS Sa179lw OX=2126819 GN=vBEcoSSa179w3YLVW_00034 PE=4 SV=1
MM1 pKa = 6.99SQWIKK6 pKa = 10.14CSEE9 pKa = 4.21MMPPTNVAVLVCVNEE24 pKa = 4.33VVQKK28 pKa = 11.14NVFCWDD34 pKa = 3.44GEE36 pKa = 4.6SWSDD40 pKa = 2.87WYY42 pKa = 11.46NEE44 pKa = 3.71YY45 pKa = 11.05DD46 pKa = 3.92EE47 pKa = 5.77LAQTTFDD54 pKa = 3.02HH55 pKa = 6.74WMPLPGPPQEE65 pKa = 4.16

Molecular weight: 7.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1MXE5|A0A2P1MXE5_9CAUD Transcriptional activator protein C1 OS=Escherichia phage vB_EcoS Sa179lw OX=2126819 GN=C1 PE=4 SV=1
MM1 pKa = 7.8AKK3 pKa = 9.86TIYY6 pKa = 9.96RR7 pKa = 11.84RR8 pKa = 11.84EE9 pKa = 4.01KK10 pKa = 10.21LEE12 pKa = 3.76QEE14 pKa = 4.24LGHH17 pKa = 7.05VGAQNFMSKK26 pKa = 7.49QARR29 pKa = 11.84NAMEE33 pKa = 4.66SIRR36 pKa = 11.84VNRR39 pKa = 11.84VVRR42 pKa = 11.84VFNGEE47 pKa = 3.69GKK49 pKa = 10.08RR50 pKa = 11.84RR51 pKa = 11.84VMDD54 pKa = 3.54EE55 pKa = 4.36LIIVFF60 pKa = 4.5

Molecular weight: 7.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

14311

31

980

166.4

18.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.776 ± 0.646

1.384 ± 0.173

6.219 ± 0.271

6.792 ± 0.322

3.606 ± 0.185

6.764 ± 0.209

1.607 ± 0.179

6.673 ± 0.269

6.275 ± 0.325

6.834 ± 0.23

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.347 ± 0.189

4.842 ± 0.193

3.382 ± 0.275

3.794 ± 0.395

5.017 ± 0.232

6.701 ± 0.343

5.765 ± 0.425

6.834 ± 0.247

1.474 ± 0.147

3.913 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski