Hymenobacter jejuensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Hymenobacter

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8A0S9|A0A5B8A0S9_9BACT GSH-S_ATP domain-containing protein OS=Hymenobacter jejuensis OX=2502781 GN=FHG12_09715 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 10.48RR3 pKa = 11.84LIDD6 pKa = 4.37ALADD10 pKa = 3.86DD11 pKa = 4.83ADD13 pKa = 4.09FFVEE17 pKa = 4.74HH18 pKa = 5.67VQITAIVFDD27 pKa = 3.77NTNDD31 pKa = 3.3VTVWATTYY39 pKa = 10.58FDD41 pKa = 3.89EE42 pKa = 4.67EE43 pKa = 4.34LHH45 pKa = 6.19FFHH48 pKa = 7.36LGLQFQYY55 pKa = 11.47LDD57 pKa = 3.79LLLRR61 pKa = 11.84LAGSRR66 pKa = 11.84AEE68 pKa = 3.96ALQEE72 pKa = 3.95EE73 pKa = 5.03VAEE76 pKa = 4.25ALATVTEE83 pKa = 4.86WPCLLEE89 pKa = 4.12YY90 pKa = 10.28TSEE93 pKa = 4.2EE94 pKa = 4.34EE95 pKa = 4.46PPVPLDD101 pKa = 3.59GVSLKK106 pKa = 10.82LSCTYY111 pKa = 10.34PADD114 pKa = 3.67TDD116 pKa = 4.06EE117 pKa = 5.81DD118 pKa = 4.62DD119 pKa = 4.28EE120 pKa = 5.45DD121 pKa = 4.4AVPHH125 pKa = 6.32NIFYY129 pKa = 11.16LEE131 pKa = 3.91GVYY134 pKa = 10.71LRR136 pKa = 11.84IEE138 pKa = 4.02PP139 pKa = 4.07

Molecular weight: 15.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8A3K3|A0A5B8A3K3_9BACT Omp85 domain-containing protein OS=Hymenobacter jejuensis OX=2502781 GN=FHG12_18210 PE=4 SV=1
MM1 pKa = 7.81DD2 pKa = 3.43TTTIFRR8 pKa = 11.84RR9 pKa = 11.84CVPRR13 pKa = 11.84QRR15 pKa = 11.84VFWVLLVCLFSFFVHH30 pKa = 6.67LGAPEE35 pKa = 3.94VSLMEE40 pKa = 4.09SRR42 pKa = 11.84NFVAARR48 pKa = 11.84EE49 pKa = 4.01MAAGGSWLIPTMNGEE64 pKa = 4.2LRR66 pKa = 11.84LAKK69 pKa = 10.23PPLPTWAVAALLKK82 pKa = 10.89VVGPTQNLAVLRR94 pKa = 11.84LPAALMATLLIFFFWGLARR113 pKa = 11.84EE114 pKa = 4.39LTRR117 pKa = 11.84EE118 pKa = 4.09QPAEE122 pKa = 3.56AAAPGRR128 pKa = 11.84TAWLSALVLGSSLLLVTTGRR148 pKa = 11.84EE149 pKa = 4.3GQWDD153 pKa = 3.47IFSNCFMVGSLWLLVRR169 pKa = 11.84GLNSAGASYY178 pKa = 11.14GRR180 pKa = 11.84FVGAGVLLGCSFLSKK195 pKa = 10.98GPVALFGLLLPFAACYY211 pKa = 9.86CIQRR215 pKa = 11.84LNPAGRR221 pKa = 11.84ARR223 pKa = 11.84LRR225 pKa = 11.84VHH227 pKa = 7.28RR228 pKa = 11.84GGLLVALLIAVLVGGAWPLYY248 pKa = 9.43IFEE251 pKa = 4.85HH252 pKa = 5.38VQPAALAVAQTEE264 pKa = 4.29VTAWRR269 pKa = 11.84EE270 pKa = 3.75RR271 pKa = 11.84HH272 pKa = 4.67VQAWWYY278 pKa = 9.1YY279 pKa = 10.24LNFPVFAGVWAVVALAALAVPYY301 pKa = 10.46ARR303 pKa = 11.84RR304 pKa = 11.84RR305 pKa = 11.84SEE307 pKa = 4.5AYY309 pKa = 8.98IPYY312 pKa = 9.65VFALGWLLVTLVLLSAVPEE331 pKa = 4.06KK332 pKa = 10.63KK333 pKa = 9.59EE334 pKa = 4.53RR335 pKa = 11.84YY336 pKa = 7.77MLPLMPPLALLMGGMLRR353 pKa = 11.84HH354 pKa = 6.28WEE356 pKa = 4.11TSRR359 pKa = 11.84TLPRR363 pKa = 11.84LRR365 pKa = 11.84PADD368 pKa = 4.06GVWLRR373 pKa = 11.84VWAGLLLLVCLLVPVAMVVIKK394 pKa = 10.9LPGFEE399 pKa = 4.13PATLRR404 pKa = 11.84FGAALGVFGSLAVLVWRR421 pKa = 11.84QGWQRR426 pKa = 11.84RR427 pKa = 11.84QPVHH431 pKa = 5.61LTGASAAGMAAVLALLMPAYY451 pKa = 9.16PVWEE455 pKa = 4.01ARR457 pKa = 11.84KK458 pKa = 9.46GDD460 pKa = 3.19GSLRR464 pKa = 11.84HH465 pKa = 6.08ISDD468 pKa = 3.0VRR470 pKa = 11.84EE471 pKa = 3.79RR472 pKa = 11.84AEE474 pKa = 4.04LQRR477 pKa = 11.84LPWVGQQEE485 pKa = 4.26VHH487 pKa = 6.95IKK489 pKa = 9.41QVWNAGRR496 pKa = 11.84SIPTWHH502 pKa = 7.81PDD504 pKa = 2.79TTAAEE509 pKa = 4.38LPSLPVVVFSSDD521 pKa = 3.12PHH523 pKa = 7.48VLEE526 pKa = 4.09KK527 pKa = 10.89LPRR530 pKa = 11.84KK531 pKa = 8.34WRR533 pKa = 11.84ARR535 pKa = 11.84LRR537 pKa = 11.84SEE539 pKa = 5.02RR540 pKa = 11.84IDD542 pKa = 3.48SFFLGRR548 pKa = 11.84TAKK551 pKa = 10.65DD552 pKa = 3.5GWWMVSLLQRR562 pKa = 11.84KK563 pKa = 7.97VNN565 pKa = 3.53

Molecular weight: 62.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4100

0

4100

1465612

25

2462

357.5

39.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.882 ± 0.045

0.721 ± 0.01

5.034 ± 0.026

5.336 ± 0.04

4.256 ± 0.021

7.386 ± 0.036

2.108 ± 0.022

4.843 ± 0.031

4.461 ± 0.034

10.593 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.968 ± 0.018

4.055 ± 0.035

4.999 ± 0.026

4.624 ± 0.028

5.767 ± 0.032

5.737 ± 0.03

6.164 ± 0.047

7.181 ± 0.028

1.266 ± 0.016

3.617 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski