Luffa aphid-borne yellows virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus; unclassified Polerovirus

Average proteome isoelectric point is 7.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8JWL6|W8JWL6_9LUTE Movement protein OS=Luffa aphid-borne yellows virus OX=1462682 PE=3 SV=2
MM1 pKa = 7.17GHH3 pKa = 6.19MLRR6 pKa = 11.84VGHH9 pKa = 6.51SGTGDD14 pKa = 3.2VDD16 pKa = 3.39NDD18 pKa = 3.34EE19 pKa = 4.14PRR21 pKa = 11.84EE22 pKa = 3.98YY23 pKa = 11.35GMSSHH28 pKa = 5.88QAAVQSWSSLTYY40 pKa = 10.77NPDD43 pKa = 2.76VDD45 pKa = 4.57EE46 pKa = 5.66IDD48 pKa = 3.87VIGEE52 pKa = 3.98EE53 pKa = 4.47EE54 pKa = 4.12EE55 pKa = 4.66VEE57 pKa = 4.34FPEE60 pKa = 4.57VQGLLKK66 pKa = 10.71SSSLYY71 pKa = 9.53YY72 pKa = 8.68QTSRR76 pKa = 11.84EE77 pKa = 4.06TXPGQSSSDD86 pKa = 3.78HH87 pKa = 6.09LCHH90 pKa = 7.29RR91 pKa = 11.84SQNLRR96 pKa = 11.84QEE98 pKa = 4.36YY99 pKa = 10.42SSPTLAIRR107 pKa = 11.84YY108 pKa = 7.51HH109 pKa = 6.71RR110 pKa = 11.84SHH112 pKa = 7.1SVFAPRR118 pKa = 11.84RR119 pKa = 11.84LLPMVAPSSISSIPLALQRR138 pKa = 11.84NSTRR142 pKa = 11.84SSIGSRR148 pKa = 11.84YY149 pKa = 7.1PTEE152 pKa = 4.01PLKK155 pKa = 10.11MLRR158 pKa = 11.84GEE160 pKa = 4.08GHH162 pKa = 6.49RR163 pKa = 11.84SEE165 pKa = 4.75EE166 pKa = 4.03RR167 pKa = 11.84SGTPRR172 pKa = 11.84AQSNSGSYY180 pKa = 8.24TKK182 pKa = 10.81EE183 pKa = 3.45MAKK186 pKa = 9.98QKK188 pKa = 10.77SLARR192 pKa = 11.84SPSQWWW198 pKa = 3.06

Molecular weight: 22.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8JD48|W8JD48_9LUTE Serine protease OS=Luffa aphid-borne yellows virus OX=1462682 PE=4 SV=2
MM1 pKa = 6.6NTAGGRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84NGSYY13 pKa = 8.59VTSRR17 pKa = 11.84TLRR20 pKa = 11.84NRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84QRR27 pKa = 11.84RR28 pKa = 11.84AARR31 pKa = 11.84VRR33 pKa = 11.84NVIPPGGGPIMVLPNLQSRR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84NRR57 pKa = 11.84RR58 pKa = 11.84NRR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84GGGVSRR69 pKa = 11.84GSGSTEE75 pKa = 3.55VFKK78 pKa = 11.12FILPDD83 pKa = 3.65LKK85 pKa = 11.09GNSAGTIKK93 pKa = 10.66FGPSLSQKK101 pKa = 10.45PEE103 pKa = 3.57FATGILKK110 pKa = 10.48SYY112 pKa = 10.74ARR114 pKa = 11.84YY115 pKa = 9.76KK116 pKa = 10.27ISQVTLSFRR125 pKa = 11.84SEE127 pKa = 3.75ASATDD132 pKa = 3.64GGALVYY138 pKa = 10.74QLDD141 pKa = 4.17TTCAATQLDD150 pKa = 3.84SKK152 pKa = 10.62LYY154 pKa = 10.27RR155 pKa = 11.84FSISNRR161 pKa = 11.84APQNVTWRR169 pKa = 11.84GAQIRR174 pKa = 11.84GEE176 pKa = 4.27EE177 pKa = 4.01WHH179 pKa = 6.5STSSEE184 pKa = 4.27QFWVLYY190 pKa = 9.6QGNGEE195 pKa = 4.18AKK197 pKa = 8.83VAGSITVTMVVNFLDD212 pKa = 4.25PKK214 pKa = 10.73

Molecular weight: 23.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3092

198

1082

515.3

57.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.05 ± 0.367

2.005 ± 0.565

4.107 ± 0.383

5.595 ± 0.426

3.784 ± 0.353

7.503 ± 0.517

2.426 ± 0.412

3.913 ± 0.474

5.045 ± 0.682

9.799 ± 1.098

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.714 ± 0.219

3.655 ± 0.277

6.339 ± 0.514

3.816 ± 0.192

6.824 ± 1.374

9.541 ± 0.782

5.563 ± 0.384

6.21 ± 0.261

1.94 ± 0.173

2.943 ± 0.121
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski