Ruegeria phage vB_RpoMi-V15

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4QGJ3|A0A2Z4QGJ3_9VIRU Capsid protein OS=Ruegeria phage vB_RpoMi-V15 OX=2218610 GN=vBRpoMiV15_04 PE=4 SV=1
MM1 pKa = 7.53LANLIMQQGGAKK13 pKa = 9.73SKK15 pKa = 10.48RR16 pKa = 11.84KK17 pKa = 9.33NRR19 pKa = 11.84NPVFTLGGTIKK30 pKa = 10.74PFGLYY35 pKa = 10.08PMFIHH40 pKa = 6.72PVLPGEE46 pKa = 4.31TLNSFTMKK54 pKa = 9.98VHH56 pKa = 6.91LASEE60 pKa = 4.17PLKK63 pKa = 11.19NPLNGAWWEE72 pKa = 3.95TWLFYY77 pKa = 11.39VKK79 pKa = 10.73LNDD82 pKa = 4.26LGPDD86 pKa = 3.45LVEE89 pKa = 4.22MFIKK93 pKa = 10.73DD94 pKa = 3.7GVSTTGHH101 pKa = 5.94TAGSSDD107 pKa = 3.21QLFFTKK113 pKa = 10.18SGQIDD118 pKa = 3.88WVRR121 pKa = 11.84KK122 pKa = 8.48CTDD125 pKa = 3.88LVHH128 pKa = 5.74SHH130 pKa = 5.77YY131 pKa = 10.46FIHH134 pKa = 7.04EE135 pKa = 4.56DD136 pKa = 3.04EE137 pKa = 4.4TARR140 pKa = 11.84TINGVPQVKK149 pKa = 10.07LNSKK153 pKa = 9.29GWWQNLMQNDD163 pKa = 3.35ADD165 pKa = 4.04AAVPTGDD172 pKa = 3.57AADD175 pKa = 4.21MYY177 pKa = 11.75AHH179 pKa = 6.43LQEE182 pKa = 4.6FAILQQMGMQEE193 pKa = 3.97MTYY196 pKa = 10.42EE197 pKa = 4.31KK198 pKa = 10.65YY199 pKa = 10.92LEE201 pKa = 4.34LFGGRR206 pKa = 11.84AAAAGQGKK214 pKa = 9.13PEE216 pKa = 4.38LLLYY220 pKa = 9.42DD221 pKa = 3.99QRR223 pKa = 11.84WKK225 pKa = 11.06KK226 pKa = 9.02PANTINPADD235 pKa = 4.1GTPSSAWVWNEE246 pKa = 3.45EE247 pKa = 4.17TKK249 pKa = 10.9LGSKK253 pKa = 9.82NKK255 pKa = 9.93RR256 pKa = 11.84FNEE259 pKa = 4.08PGFVIAFQTIRR270 pKa = 11.84PKK272 pKa = 10.15MFNGAVRR279 pKa = 11.84TSLVGNMWGFKK290 pKa = 9.68DD291 pKa = 3.78WYY293 pKa = 9.35PAYY296 pKa = 9.68TLDD299 pKa = 5.74DD300 pKa = 3.51PTATVKK306 pKa = 10.07TIANNDD312 pKa = 3.65PVFEE316 pKa = 4.3ASAYY320 pKa = 5.27TTGGTEE326 pKa = 5.02LIYY329 pKa = 10.97DD330 pKa = 4.11HH331 pKa = 7.49SDD333 pKa = 2.99LLAQGEE339 pKa = 4.35QFINDD344 pKa = 3.67YY345 pKa = 10.4APPYY349 pKa = 10.15ALPLSSGMVVEE360 pKa = 5.19GGGTPEE366 pKa = 4.06SFRR369 pKa = 11.84GEE371 pKa = 4.44YY372 pKa = 8.24CTSADD377 pKa = 3.69VDD379 pKa = 4.08ALFQDD384 pKa = 4.5GAKK387 pKa = 9.13TRR389 pKa = 11.84AFYY392 pKa = 10.96DD393 pKa = 4.79GIAFCNILGHH403 pKa = 6.09VADD406 pKa = 4.43NTPLKK411 pKa = 10.84

Molecular weight: 45.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4QG15|A0A2Z4QG15_9VIRU Replication protein OS=Ruegeria phage vB_RpoMi-V15 OX=2218610 GN=vBRpoMiV15_02 PE=4 SV=1
MM1 pKa = 7.54EE2 pKa = 5.09FFRR5 pKa = 11.84YY6 pKa = 9.79SEE8 pKa = 4.29FQCKK12 pKa = 9.46CGCGMLPQPFVMDD25 pKa = 3.79NLDD28 pKa = 3.87HH29 pKa = 6.79IRR31 pKa = 11.84RR32 pKa = 11.84ALRR35 pKa = 11.84VPLVIHH41 pKa = 6.46SGARR45 pKa = 11.84CARR48 pKa = 11.84HH49 pKa = 5.09NARR52 pKa = 11.84VGGALLSQHH61 pKa = 5.21VKK63 pKa = 10.27GRR65 pKa = 11.84AVDD68 pKa = 3.48IRR70 pKa = 11.84WPEE73 pKa = 3.81GHH75 pKa = 6.35QSALLGLLQRR85 pKa = 11.84NGVRR89 pKa = 11.84GIGIYY94 pKa = 8.56PTFVHH99 pKa = 6.87ADD101 pKa = 3.01WRR103 pKa = 11.84EE104 pKa = 3.9GPSAFWVRR112 pKa = 11.84PEE114 pKa = 3.98GARR117 pKa = 11.84TT118 pKa = 3.29

Molecular weight: 13.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1294

118

451

323.5

35.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.505 ± 0.618

1.236 ± 0.475

5.255 ± 0.614

4.714 ± 0.074

3.941 ± 0.752

9.505 ± 0.976

3.246 ± 0.398

4.019 ± 0.492

5.873 ± 0.991

7.651 ± 0.346

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.318 ± 0.332

3.709 ± 0.669

6.646 ± 0.502

3.246 ± 0.442

7.419 ± 1.756

5.1 ± 0.436

5.41 ± 0.735

5.719 ± 0.331

2.087 ± 0.377

3.4 ± 0.353
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski