Muribaculaceae bacterium Isolate-037 (Harlan)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Muribaculaceae; unclassified Muribaculaceae

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3984 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N2MFS3|A0A3N2MFS3_9BACT Uncharacterized protein OS=Muribaculaceae bacterium Isolate-037 (Harlan) OX=2486461 GN=EEL33_04705 PE=4 SV=1
MM1 pKa = 7.1SAAVSMAAPPQTGFYY16 pKa = 10.58LSGFNGEE23 pKa = 4.36SEE25 pKa = 4.16ATEE28 pKa = 4.46SNRR31 pKa = 11.84LTYY34 pKa = 10.72VPGNEE39 pKa = 3.85EE40 pKa = 4.71DD41 pKa = 3.83EE42 pKa = 5.04DD43 pKa = 3.7EE44 pKa = 5.09GIYY47 pKa = 10.61RR48 pKa = 11.84FINDD52 pKa = 3.14NFVISEE58 pKa = 4.16CQNGFFAIGEE68 pKa = 4.27EE69 pKa = 4.38GLKK72 pKa = 10.86LGFNVDD78 pKa = 3.11NDD80 pKa = 3.97FGFSNMVNQYY90 pKa = 9.23ATMTSLAEE98 pKa = 4.01NGPAINCEE106 pKa = 4.2LPAGTYY112 pKa = 10.06KK113 pKa = 10.91VILASMQEE121 pKa = 4.21EE122 pKa = 4.89GDD124 pKa = 3.87PMSWTMMFQSLNNDD138 pKa = 3.71DD139 pKa = 4.41EE140 pKa = 5.78AISYY144 pKa = 9.04YY145 pKa = 10.85LIGFNGNDD153 pKa = 4.02EE154 pKa = 4.47LSQDD158 pKa = 3.86NIFVAEE164 pKa = 4.09EE165 pKa = 4.31SEE167 pKa = 4.44DD168 pKa = 3.8GVIYY172 pKa = 8.53TYY174 pKa = 11.01PKK176 pKa = 10.54FLVEE180 pKa = 4.25DD181 pKa = 3.99CADD184 pKa = 3.54GFFVANSKK192 pKa = 11.24DD193 pKa = 3.66DD194 pKa = 3.93TLLGAAARR202 pKa = 11.84SDD204 pKa = 3.24KK205 pKa = 10.51VTDD208 pKa = 3.75EE209 pKa = 4.47NPFASLEE216 pKa = 4.1NSGDD220 pKa = 3.72AVQSSLTPGYY230 pKa = 8.77YY231 pKa = 8.94TVNFISMNGLNMISFLRR248 pKa = 11.84CEE250 pKa = 4.5DD251 pKa = 3.26QTPADD256 pKa = 3.64EE257 pKa = 4.3SVYY260 pKa = 11.14YY261 pKa = 10.98LLGFNGIEE269 pKa = 4.06NPTDD273 pKa = 3.12GVKK276 pKa = 10.43FVRR279 pKa = 11.84SVEE282 pKa = 3.94EE283 pKa = 3.56SSYY286 pKa = 11.16EE287 pKa = 3.95DD288 pKa = 3.96EE289 pKa = 4.7EE290 pKa = 4.37TGEE293 pKa = 4.46MISEE297 pKa = 4.16TVITYY302 pKa = 7.36TLKK305 pKa = 10.82NFDD308 pKa = 4.12IKK310 pKa = 11.07SCSEE314 pKa = 4.01DD315 pKa = 3.24GFIVTTEE322 pKa = 4.37DD323 pKa = 3.11GGFSYY328 pKa = 10.93GLSTMFVPMLGNTISLDD345 pKa = 3.71SPFGMMGIYY354 pKa = 9.85GKK356 pKa = 9.23PYY358 pKa = 10.3KK359 pKa = 10.32SALPEE364 pKa = 3.88GKK366 pKa = 10.18YY367 pKa = 10.57DD368 pKa = 3.89INFSTSDD375 pKa = 3.23TSASISFLPSEE386 pKa = 4.45DD387 pKa = 3.85SGVEE391 pKa = 4.18SIMTGDD397 pKa = 3.3NSEE400 pKa = 3.93AVYY403 pKa = 11.16YY404 pKa = 9.94DD405 pKa = 3.35LQGRR409 pKa = 11.84RR410 pKa = 11.84MINPDD415 pKa = 2.93KK416 pKa = 10.93GIYY419 pKa = 9.81ILVKK423 pKa = 9.81NGEE426 pKa = 3.81VSKK429 pKa = 10.69IVKK432 pKa = 9.63

Molecular weight: 47.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N2M8V4|A0A3N2M8V4_9BACT DUF4099 domain-containing protein OS=Muribaculaceae bacterium Isolate-037 (Harlan) OX=2486461 GN=EEL33_09520 PE=4 SV=1
MM1 pKa = 7.43NGLEE5 pKa = 4.39KK6 pKa = 10.56ALQTLHH12 pKa = 7.03GYY14 pKa = 9.49GSRR17 pKa = 11.84HH18 pKa = 4.92SAIDD22 pKa = 3.05TGIQLFVTLGFIVAVVSVPTVDD44 pKa = 3.51PARR47 pKa = 11.84LIWFFAFPVIMAEE60 pKa = 4.03ALRR63 pKa = 11.84IGYY66 pKa = 9.16AHH68 pKa = 6.41VFCRR72 pKa = 11.84SLWLLPFIILIGIFNPIIDD91 pKa = 4.6RR92 pKa = 11.84QPAWEE97 pKa = 4.5IGNIVISKK105 pKa = 9.42GWISFISVVLRR116 pKa = 11.84GLLSVQALLILVDD129 pKa = 3.56SCGFNNICRR138 pKa = 11.84VLHH141 pKa = 5.47GVGVPSVLVTQLQMLYY157 pKa = 10.57RR158 pKa = 11.84FMGVLMEE165 pKa = 4.73EE166 pKa = 3.97ALIMHH171 pKa = 7.97RR172 pKa = 11.84ARR174 pKa = 11.84QSRR177 pKa = 11.84GFGRR181 pKa = 11.84KK182 pKa = 8.98SYY184 pKa = 9.14PLKK187 pKa = 10.07MWATFAGQLLMRR199 pKa = 11.84SLDD202 pKa = 3.29RR203 pKa = 11.84ASRR206 pKa = 11.84INRR209 pKa = 11.84AMLARR214 pKa = 11.84VYY216 pKa = 10.47DD217 pKa = 3.58SKK219 pKa = 11.04RR220 pKa = 11.84IHH222 pKa = 6.1KK223 pKa = 10.27AKK225 pKa = 10.26RR226 pKa = 11.84PKK228 pKa = 9.31LTRR231 pKa = 11.84NDD233 pKa = 4.01WIYY236 pKa = 10.81LAAWTTIILFIRR248 pKa = 11.84FIDD251 pKa = 3.56ISAIIDD257 pKa = 3.35KK258 pKa = 9.85WLSTHH263 pKa = 6.23LTT265 pKa = 3.57

Molecular weight: 30.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3984

0

3984

1419065

29

6922

356.2

40.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.284 ± 0.047

1.202 ± 0.013

6.254 ± 0.028

6.369 ± 0.041

4.345 ± 0.027

6.96 ± 0.04

1.795 ± 0.017

6.962 ± 0.041

6.121 ± 0.034

8.599 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.812 ± 0.019

5.2 ± 0.032

3.934 ± 0.022

2.883 ± 0.023

5.106 ± 0.036

6.749 ± 0.033

5.499 ± 0.028

6.402 ± 0.032

1.318 ± 0.016

4.207 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski