Rhodothalassium salexigens DSM 2132

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodothalassiales; Rhodothalassiaceae; Rhodothalassium; Rhodothalassium salexigens

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2870 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R2PGQ9|A0A4R2PGQ9_RHOSA Membrane fusion protein (Multidrug efflux system) OS=Rhodothalassium salexigens DSM 2132 OX=1188247 GN=EV659_105205 PE=3 SV=1
MM1 pKa = 7.56SNRR4 pKa = 11.84YY5 pKa = 8.41HH6 pKa = 6.22ATPYY10 pKa = 10.51DD11 pKa = 3.36ISATGFYY18 pKa = 10.27FDD20 pKa = 4.69TYY22 pKa = 11.24EE23 pKa = 4.13EE24 pKa = 4.53YY25 pKa = 8.95QTRR28 pKa = 11.84AAAHH32 pKa = 6.14RR33 pKa = 11.84NEE35 pKa = 4.75HH36 pKa = 6.28GDD38 pKa = 3.42PVEE41 pKa = 4.05EE42 pKa = 4.55YY43 pKa = 10.0EE44 pKa = 4.42IQFIDD49 pKa = 3.63GDD51 pKa = 4.06SYY53 pKa = 11.98RR54 pKa = 11.84LFEE57 pKa = 5.54AIGVNQANLKK67 pKa = 10.14DD68 pKa = 3.23WFEE71 pKa = 4.47RR72 pKa = 11.84FEE74 pKa = 6.35DD75 pKa = 3.69MDD77 pKa = 5.31DD78 pKa = 4.16DD79 pKa = 4.31EE80 pKa = 4.82AVKK83 pKa = 10.6LIILIEE89 pKa = 3.99HH90 pKa = 6.84FGYY93 pKa = 10.38SVEE96 pKa = 4.44DD97 pKa = 3.49ALDD100 pKa = 4.74RR101 pKa = 11.84IDD103 pKa = 5.54DD104 pKa = 3.65LHH106 pKa = 8.41LFEE109 pKa = 5.02GTAIEE114 pKa = 4.18YY115 pKa = 10.57AEE117 pKa = 4.43EE118 pKa = 4.75FIEE121 pKa = 4.38STGMLDD127 pKa = 4.48QMPEE131 pKa = 3.46NLRR134 pKa = 11.84YY135 pKa = 10.31YY136 pKa = 10.6FDD138 pKa = 4.2SEE140 pKa = 4.05AFARR144 pKa = 11.84DD145 pKa = 3.49MVLGGDD151 pKa = 3.49ITQVEE156 pKa = 4.57IDD158 pKa = 3.64GHH160 pKa = 6.5SYY162 pKa = 10.31IADD165 pKa = 3.62GVV167 pKa = 3.73

Molecular weight: 19.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R2PMF5|A0A4R2PMF5_RHOSA Photosystem II stability/assembly factor-like uncharacterized protein OS=Rhodothalassium salexigens DSM 2132 OX=1188247 GN=EV659_10475 PE=4 SV=1
MM1 pKa = 7.72PLALTIRR8 pKa = 11.84SIAVKK13 pKa = 9.45RR14 pKa = 11.84TYY16 pKa = 10.23QPSRR20 pKa = 11.84LVRR23 pKa = 11.84KK24 pKa = 9.21RR25 pKa = 11.84RR26 pKa = 11.84HH27 pKa = 4.57GFRR30 pKa = 11.84ARR32 pKa = 11.84KK33 pKa = 7.82ATQAGQKK40 pKa = 9.38ILNARR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84KK48 pKa = 9.34GRR50 pKa = 11.84KK51 pKa = 8.86KK52 pKa = 10.58LSAA55 pKa = 3.95

Molecular weight: 6.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2870

0

2870

988374

29

5271

344.4

37.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.411 ± 0.1

0.742 ± 0.014

6.872 ± 0.039

5.219 ± 0.044

3.314 ± 0.035

8.869 ± 0.043

2.034 ± 0.024

3.958 ± 0.034

2.385 ± 0.036

10.225 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.151 ± 0.023

2.108 ± 0.033

5.641 ± 0.043

3.142 ± 0.031

8.116 ± 0.046

4.379 ± 0.034

5.455 ± 0.038

7.604 ± 0.035

1.303 ± 0.018

2.072 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski